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Journal of Nanoparticle Research
Erschienen in: Journal of Nanoparticle Research 8/2010

01.10.2010 | Research Paper

Covalent immobilization of invertase on PAMAM-dendrimer modified superparamagnetic iron oxide nanoparticles

verfasst von: K. Uzun, E. Çevik, M. Şenel, H. Sözeri, A. Baykal, M. F. Abasıyanık, M. S. Toprak

Erschienen in: Journal of Nanoparticle Research | Ausgabe 8/2010

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Abstract

In this study, polyamidoamine (PAMAM) dendrimer was synthesized on the surface of superparamagnetite nanoparticles to enhance invertase immobilization. The amount of immobilized enzyme on the surface-hyperbranched magnetite nanoparticle was up to 2.5 times (i.e., 250%) as much as that of magnetite nanoparticle modified with only amino silane. Maximum reaction rate (V max) and Michaelis–Menten constant (K m) were determined for the free and immobilized enzymes. Various characteristics of immobilized invertase such as; the temperature activity, thermal stability, operational stability, and storage stability were evaluated and results revealed that stability of the enzyme is improved upon immobilization.
Vorheriger Artikel Nanoparticles of nickel oxide: growth and organization on zinc-substituted anionic clay matrix by one-pot route at room temperature
Nächster Artikel Phase transition of BiVO4 nanoparticles in molten salt and the enhancement of visible-light photocatalytic activity

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Metadaten
Titel
Covalent immobilization of invertase on PAMAM-dendrimer modified superparamagnetic iron oxide nanoparticles
verfasst von
K. Uzun
E. Çevik
M. Şenel
H. Sözeri
A. Baykal
M. F. Abasıyanık
M. S. Toprak
Publikationsdatum
01.10.2010
Verlag
Springer Netherlands
Erschienen in
Journal of Nanoparticle Research / Ausgabe 8/2010
Print ISSN: 1388-0764
Elektronische ISSN: 1572-896X
DOI
https://doi.org/10.1007/s11051-010-9902-9

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