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01.04.2014 | Research Paper

Sonicated and stirred copper oxide nanoparticles induce similar toxicity and pro-inflammatory response in N-hTERT keratinocytes and SZ95 sebocytes

verfasst von: Jean-Pascal Piret, Jorge Mejia, Stéphane Lucas, Christos C. Zouboulis, Christelle Saout, Olivier Toussaint

Erschienen in: Journal of Nanoparticle Research | Ausgabe 4/2014

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Abstract

The potential toxic and pro-inflammatory effects of rod-shaped copper oxide (CuO) nanoparticles (NPs; 10 ± 3 nm in thickness and 74 ± 17 nm in length) were studied on N-hTERT keratinocytes and SZ95 sebocytes and on reconstructed human epidermis. Non-sonicated and sonicated CuO NPs induced similar cellular toxicity. The toxic effect of CuO NPs (non-sonicated and sonicated) was more pronounced in keratinocytes than in sebocytes. Pro-oxidant effects of CuO NPs were demonstrated by showing increase in the production of reactive oxygen species and decrease of cellular glutathione. In addition, DNA-binding activities suggested that redox-sensitive transcription factors Nrf2 and NF-κB were implicated in the response of keratinocytes to CuO NPs. Transcriptomic analysis showed an increase in the abundance of transcript species coding for pro-inflammatory interleukins (e.g. IL-8 and IL-1α) and chemokines. In reconstituted human epidermis exposed topically to raw CuO NPs, no effect on the integrity, viability and inflammatory response was noticed.

Vorheriger Artikel Curcumin-sensitized TiO2 for enhanced photodegradation of dyes under visible light

Nächster Artikel Comparative in vivo assessment of the subacute toxicity of gold and silver nanoparticles

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Titel: Sonicated and stirred copper oxide nanoparticles induce similar toxicity and pro-inflammatory response in N-hTERT keratinocytes and SZ95 sebocytes
verfasst von: Jean-Pascal Piret
Jorge Mejia
Stéphane Lucas
Christos C. Zouboulis
Christelle Saout
Olivier Toussaint
Publikationsdatum: 01.04.2014
Verlag: Springer Netherlands
Erschienen in: Journal of Nanoparticle Research / Ausgabe 4/2014
Print ISSN: 1388-0764
Elektronische ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-014-2337-y

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