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Natural Deep Eutectic Solvents as Alternatives for Extracting Phlorotannins from Brown Algae

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Pharmaceutical Chemistry Journal Aims and scope

Natural deep eutectic solvents (NADES) are promising green solvents for extracting biologically active compounds from plant raw material. This is the first study in which NADES were used to extract phlorotannins from brown algae Fucus vesiculosus L. and Ascophyllum nodosum (L.) Le Jolis. The extraction efficiency of polyphenols was evaluated using 10 NADES based on choline chloride, lactic acid, betaine, and glucose in various mole ratios. The effect of H2O in aqueous solutions of NADES on extraction of phlorotannins was studied. Algae were extracted by maceration for 120 min at 50°C with a 1:5 raw-material:extractant ratio. Phlorotannins were quantified by spectrophotometry using the Folin–Ciocalteu method. The maximum extraction of phlorotannins (60 – 72%) was achieved using aqueous NADES solutions (50 – 70%) based on choline chloride with added lactic or malic acid and also on malic acid and betaine. The NADES had efficiencies comparable to Me2CO and EtOH.

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Acknowledgments

The work was sponsored by a State Task of MMBI, KSC, RAS, Ministry of Education and Science of the RF (State Reg. No. 01201453843). NADES syntheses were supported by RFBR Grant No. 17-44-510487-reg sever a.

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Authors and Affiliations

  1. Murmansk Marine Biological Institute, Kola Scientific Center, Russian Academy of Sciences, 17 Vladimirskaya St., Murmansk, 183010, Russia

    E. D. Obluchinskaya & A. V. Daurtseva

  2. St. Petersburg Institute of Pharmacy, Bldg. 245, Kuzmolovo, Vsevolozhsk District, Leningrad Oblast, 188663, Russia

    O. N. Pozharitskaya & A. N. Shikov

  3. St. Petersburg State Chemical Pharmaceutical University, 14 Prof. Popov St., St. Petersburg, 197376, Russia

    E. V. Flisyuk & A. N. Shikov

Authors
  1. E. D. Obluchinskaya
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  2. A. V. Daurtseva
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  3. O. N. Pozharitskaya
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  4. E. V. Flisyuk
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  5. A. N. Shikov
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Correspondence to E. D. Obluchinskaya.

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Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 53, No. 3, pp. 45 – 49, March, 2019.

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Obluchinskaya, E.D., Daurtseva, A.V., Pozharitskaya, O.N. et al. Natural Deep Eutectic Solvents as Alternatives for Extracting Phlorotannins from Brown Algae. Pharm Chem J 53, 243–247 (2019). https://doi.org/10.1007/s11094-019-01987-0

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  • DOI: https://doi.org/10.1007/s11094-019-01987-0

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