ABSTRACT
Purpose
Cationic solid lipid nanoparticles (SLN) have established themselves during the past decades. They can efficiently bind DNA directly via ionic interaction and mediate gene transfection. One major problem with SLN is the lack of cell-targeting ability. In the present study, a mannan-based PE-grafted ligand was synthesized and used for the surface modification of DNA-loaded cationic SLN to prepare Man-SLN-DNA.
Methods
For in vitro test, the cytotoxicity and transfection investigation was carried out on murine macrophage cell line RAW 264.7. For in vivo evaluation, Man-SLN-DNA was delivered into the lung of the rats, and the alveolar macrophages (AM) were isolated for the fluorescence determination of transfection efficiency.
Results
When compared with non-modified SLN-DNA and Lipofectamine 2000-DNA, Man-SLN-DNA produced the highest gene expressions, especially in vivo.
Conclusion
These results demonstrated the active targeting ability of this kind of mannan-modified DNA-loaded vehicles, which may have great potential for targeted gene delivery.
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ACKNOWLEDGMENTS
The authors gratefully acknowledge Dr. Laird Forrest (The Department of Pharmaceutical Chemistry, School of Pharmacy, University of Kansas, USA) for language editing. The authors thank Mr. Tianliang Sun (Shanghai Institute for Biological Sciences, China) and Mr. Qiangjun Sui (Institute of Immunopharmacology and Immunotherapy, Shandong University, China) for the flow cytometry. The work was supported by Program for New Century Excellent Talents in University (NCET-08-0334), and the National Natural Science Foundation of China, No. 30572267.
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Yu, W., Liu, C., Liu, Y. et al. Mannan-Modified Solid Lipid Nanoparticles for Targeted Gene Delivery to Alveolar Macrophages. Pharm Res 27, 1584–1596 (2010). https://doi.org/10.1007/s11095-010-0149-z
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DOI: https://doi.org/10.1007/s11095-010-0149-z