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Auxin signaling participates in the adaptative response against oxidative stress and salinity by interacting with redox metabolism in Arabidopsis

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Abstract

Auxin regulates gene expression through direct physical interaction with TIR1/AFB receptor proteins during different processes of growth and development in plants. Here we report the contribution of auxin signaling pathway to the adaptative response against abiotic stress in Arabidopsis. Phenotypic characterization of tir1/afb auxin receptor mutants indicates a differential participation of each member under abiotic stress. In particular, tir1 afb2 and tir1 afb3 mutants resulted more tolerant to oxidative stress. In addition, tir1 afb2 showed increased tolerance against salinity measured as chlorophyll content, germination rate and root elongation compared with wild-type plants. Furthermore, tir1 afb2 displayed a reduced accumulation of hydrogen peroxide and superoxide anion, as well as enhanced antioxidant enzymes activities under stress. A higher level of ascorbic acid was detected in tir1 afb2 compared with wild-type plants. Thus, adaptation to salinity in Arabidopsis may be mediated in part by an auxin/redox interaction.

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Abbreviations

ROS:

Reactive oxygen species

MV:

Methyl viologen

H2O2 :

Hydrogen peroxide

O2 − :

Superoxide anion

AA:

Ascorbate

DHA:

Dihydroascorbate

CAT:

Catalase

APX:

Ascorbate peroxidase

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Acknowledgments

We thank Dr. Mark Estelle for the mutants and helpful discussions concerning on the mechanism of auxin perception and Gustavo Gergoff for his critical reading of the manuscript. This work was supported by grants from CONICET, Universidad Nacional de Mar del Plata and ANPCyT. C.C, M.C.T. and C.G.B. are researchers from CONICET. M.J.I and S.D are postgraduate fellows from the same Institution.

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Authors and Affiliations

  1. Instituto de Investigaciones Biológicas, UE-CONICET-UNMdP, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Funes 3250, CC 1245, 7600, Mar del Plata, Argentina

    María José Iglesias, María Cecilia Terrile, Sebastián D’Ippólito & Claudia Anahí Casalongué

  2. Instituto de Fisiología Vegetal. Facultad de Ciencias Naturales, Universidad Nacional de La Plata-CCT La Plata CONICET, CC 327, 1900, La Plata, Argentina

    Carlos Guillermo Bartoli

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  1. María José Iglesias
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  2. María Cecilia Terrile
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  3. Carlos Guillermo Bartoli
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  4. Sebastián D’Ippólito
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  5. Claudia Anahí Casalongué
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Corresponding author

Correspondence to Claudia Anahí Casalongué.

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Phenotypic analysis of single mutants, tir1 and afb2 under stress

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Iglesias, M.J., Terrile, M.C., Bartoli, C.G. et al. Auxin signaling participates in the adaptative response against oxidative stress and salinity by interacting with redox metabolism in Arabidopsis . Plant Mol Biol 74, 215–222 (2010). https://doi.org/10.1007/s11103-010-9667-7

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