Abstract
The root endophytic fungus Piriformospora indica enhances plant adaptation to environmental stress based on general and non-specific plant species mechanisms. In the present study, we integrated the ionomics, metabolomics, and transcriptomics data to identify the genes and metabolic regulatory networks conferring salt tolerance in P. indica-colonized barley plants. To this end, leaf samples were harvested at control (0 mM NaCl) and severe salt stress (300 mM NaCl) in P. indica-colonized and non-inoculated barley plants 4 weeks after fungal inoculation. The metabolome analysis resulted in an identification of a signature containing 14 metabolites and ions conferring tolerance to salt stress. Gene expression analysis has led to the identification of 254 differentially expressed genes at 0 mM NaCl and 391 genes at 300 mM NaCl in P. indica-colonized compared to non-inoculated samples. The integration of metabolome and transcriptome analysis indicated that the major and minor carbohydrate metabolism, nitrogen metabolism, and ethylene biosynthesis pathway might play a role in systemic salt-tolerance in leaf tissue induced by the root-colonized fungus.
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We would like to thank Melanie Ruff at IPK-Gatersleben for the excellent technical assistance.
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MG prepared the plant samples; MRG, MRH and MG performed metabolome analysis; PS performed macroarray analysis; MRG and BK analyzed the data; GHS designed the experiment; MRG, BK and GHS wrote the manuscript.
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Supplementary Fig. S1
Difference in plant dry weight between Piriformospora indica root-colonized and non-inoculated barley plants four weeks post inoculation in 0 mM NaCl (green color) and 300 mM NaCl (red color) conditions. Bars indicate standard error values for each treatment (n = 5). (TIFF 3686 kb)
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Ghaffari, M.R., Ghabooli, M., Khatabi, B. et al. Metabolic and transcriptional response of central metabolism affected by root endophytic fungus Piriformospora indica under salinity in barley. Plant Mol Biol 90, 699–717 (2016). https://doi.org/10.1007/s11103-016-0461-z
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DOI: https://doi.org/10.1007/s11103-016-0461-z