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Role of aluminum-binding ligands in aluminum resistance of Eucalyptus camaldulensis and Melaleuca cajuputi

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Abstract

We investigated the roles of Al-binding ligands in Al exclusion from roots and in internal Al detoxification in roots as Al resistance mechanisms in two Al-resistant Myrtaceae trees, Eucalyptus camaldulensis Dehnh. and Melaleuca cajuputi Powell. The amounts of ligands secreted from roots and contained in root tips of these species were compared with those of an Al-sensitive species, Melaleuca bracteata F. Muell., after the roots were exposed to 0 or 1 mM AlCl3 solution. Secretion of well-known ligands (citrate, oxalate, and malate) from roots under Al treatment was low in all species. However, in E. camaldulensis, the Al-binding capacity of root exudates under Al treatment was considerable and was higher than that in M. bracteata. Gel filtration chromatography revealed that a low-molecular-weight Al-binding ligand was secreted from roots in response to Al only in E. camaldulensis. On the other hand, the Al-binding capacity of cell sap in root tips under Al treatment was similar for the resistant and sensitive species. These results suggest that Al exclusion by secretion of the unknown low-molecular-weight Al-binding ligand from roots contributes to the Al resistance of E. camaldulensis, whereas M. cajuputi has developed Al-resistance mechanisms other than secretion of ligands from roots or concentration of internal ligands in root tips.

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Acknowledgments

We thank Dr. Kenji Iiyama and Dr. Kyoko Katsumata of the University of Tokyo for their suggestions on the gel filtration chromatography. We are grateful to Dr. Hiroki Osawa of the University of Tokyo for his critical review of the manuscript. This study was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan and by the Global Environment Research Fund from the Ministry of the Environment of Japan.

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  1. Ko Tahara

    Present address: Department of Molecular and Cell Biology, Forestry and Forest Products Research Institute, 1 Matsunosato, Tsukuba, Ibaraki, 305-8687, Japan

Authors and Affiliations

  1. Asian Natural Environmental Science Center, the University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

    Ko Tahara, Mariko Norisada, Takashi Yamanoshita & Katsumi Kojima

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  1. Ko Tahara
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  2. Mariko Norisada
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Correspondence to Ko Tahara.

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Responsible Editor: Thomas B. Kinraide.

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Tahara, K., Norisada, M., Yamanoshita, T. et al. Role of aluminum-binding ligands in aluminum resistance of Eucalyptus camaldulensis and Melaleuca cajuputi . Plant Soil 302, 175–187 (2008). https://doi.org/10.1007/s11104-007-9464-5

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