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Effect of the presence of the plant growth promoting rhizobacterium (PGPR) Chryseobacterium balustinum Aur9 and salt stress in the pattern of flavonoids exuded by soybean roots

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Abstract

In this work we studied how biotic and abiotic stresses can alter the pattern of flavonoids exuded by Osumi soybean roots. A routine method was developed for the detection and characterization of the flavonoids present in soybean root exudates using HPLC-MS/MS. Then, a systematic screening of the flavonoids exuded under biotic stress, the presence of a plant growth promoting rhizobacterium, and salt stress was carried out. Results obtained indicate that the presence of Chryseobacterium balustinum Aur9 or 50 mM NaCl changes qualitatively the pattern of flavonoids exuded when compared to control conditions. Thus, in the presence of C. balustinum Aur9, soybean roots did not exude quercetin and naringenin and, under salt stress, flavonoids daidzein and naringenin could not be detected. Soybean root exudates obtained under saline conditions showed a diminished capacity to induce the expression of the nodA gene in comparison to the exudates obtained in the absence of salt. Moreover, lipochitooligosaccharides (LCOs) were not detected or weakly detected when Sinorhizobium fredii SMH12 was grown in the exudates obtained under salt stress conditions or under salt stress in the presence of C. balustinum Au9, respectively.

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Acknowledgments

This work was partially supported by grants AGL2005-07923-C05 and AGL2006-13758-C05/AGR of the Spanish Ministerio de Ciencia e Innovación. M. S. Dardanelli is a member of the Research Career of CONICET, Argentina.

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Authors and Affiliations

  1. Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Sevilla, c/ Profesor García González 2, 41012, Sevilla, Spain

    Marta S. Dardanelli, Hamid Manyani & Manuel Megías

  2. Departamento de Química Orgánica, Facultad de Química, Universidad de Sevilla, c/ Profesor García González, 41012, Sevilla, Spain

    Sergio González-Barroso, Miguel A. Rodríguez-Carvajal & Antonio M. Gil-Serrano

  3. Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla, Avenida Reina Mercedes 6, 41012, Sevilla, Spain

    Maria R. Espuny, Francisco Javier López-Baena, Ramon A. Bellogín & Francisco J. Ollero

  4. Departamento de Biología Molecular, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta Nacional N° 36 km 601, Cp X5804BYA, Córdoba, Argentina

    Marta S. Dardanelli

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  1. Marta S. Dardanelli
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  2. Hamid Manyani
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  3. Sergio González-Barroso
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  4. Miguel A. Rodríguez-Carvajal
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  5. Antonio M. Gil-Serrano
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  6. Maria R. Espuny
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  7. Francisco Javier López-Baena
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  8. Ramon A. Bellogín
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  9. Manuel Megías
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  10. Francisco J. Ollero
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Correspondence to Francisco J. Ollero.

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Responsible Editor: Peter A.H. Bakker.

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Dardanelli, M.S., Manyani, H., González-Barroso, S. et al. Effect of the presence of the plant growth promoting rhizobacterium (PGPR) Chryseobacterium balustinum Aur9 and salt stress in the pattern of flavonoids exuded by soybean roots. Plant Soil 328, 483–493 (2010). https://doi.org/10.1007/s11104-009-0127-6

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