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Rhizobacterial inoculants can improve nickel phytoextraction by the hyperaccumulator Alyssum pintodasilvae

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Abstract

Aim

Rhizobacteria can influence plant growth and metal accumulation. The aim of this study was to evaluate the effect of rhizobacterial inoculants on the Ni phytoextraction efficiency of the Ni-hyperaccumulator Alyssum pintodasilvae.

Method

In a preliminary screening 15 metal-tolerant bacterial strains were tested for their plant growth promoting (PGP) capacity or effect on Ni bioaccumulation. Strains were selected for their Ni tolerance, plant growth promoting traits and Ni solubilizing capacity. In a re-inoculation experiment five of the previously screened bacterial isolates were used to inoculate A. pintodasilvae in two contrasting Ni-rich soils (a serpentine (SP) soil and a sewage sludge-affected agricultural (LF) soil).

Results

Plant growth was greater in serpentine soil (where it grows naturally) than in the LF soil, probably due to Cd phytotoxicity. Rhizobacterial inoculants influenced plant growth and Ni uptake and accumulation, but the effect of the strains was dependent upon soil type. The increase in plant biomass and/or Ni accumulation significantly promoted shoot Ni removal.

Conclusion

One strain (Arthrobacter nicotinovorans SA40) was able to promote plant growth and phytoextraction of Ni in both soil types and could be a useful candidate for future field-based trials.

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Acknowledgments

This work was supported by the Spanish Ministerio de Economía e Competitividad (CTM2012-39904-C02-01) and FEDER, the Fundación Mapfre (Ayudas a la Investigación 2012), and by the 7th Framework Program of the European Commission (FP7-KBBE-266124, GREENLAND).

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Author notes

  1. C. Becerra-Castro

    Present address: CBQF/Escola Superior de Biotecnologia, Universidade Católica Portuguesa, 4200-072, Porto, Portugal

Authors and Affiliations

  1. Instituto de Investigacións Agrobiolóxicas de Galicia (IIAG), CSIC, Apdo. 122, Santiago de Compostela, 15780, Spain

    M. I. Cabello-Conejo, C. Becerra-Castro, A. Prieto-Fernández, A. Saavedra-Ferro & P. S. Kidd

  2. Departamento de Edafoloxía e Química Agrícola, Facultade de Bioloxía, Universidade de Santiago de Compostela, Santiago de Compostela, 15782, Spain

    C. Monterroso

  3. UMR BIOGECO INRA 1202, University of Bordeaux 1, Bât B2, RdC Est, avenue des Facultés, 33405, Talence, France

    M. Mench

  4. INRA, UMR BIOGECO 1202, 69 route d’Arcachon, 33612, Cestas, France

    M. Mench

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  1. M. I. Cabello-Conejo
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Correspondence to P. S. Kidd.

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Responsible Editor: Juan Barcelo.

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Fig. 1S

Concentrations of macro- and micro-nutrients in the shoots (mean ±SE) of Alyssum pintodasilvae grown in sand/perlite mixtures and inoculated with 15 rhizobacterial strains. Values of non-inoculated controls are indicated by a continuous line (±SE (broken lines)). Asterisks indicate significant differences from the control (p <0.05) (PNG 386 kb)

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Cabello-Conejo, M.I., Becerra-Castro, C., Prieto-Fernández, A. et al. Rhizobacterial inoculants can improve nickel phytoextraction by the hyperaccumulator Alyssum pintodasilvae . Plant Soil 379, 35–50 (2014). https://doi.org/10.1007/s11104-014-2043-7

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