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Elemental distribution by cryo-micro-PIXE in the zinc and cadmium hyperaccumulator Sedum plumbizincicola grown naturally

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Abstract

Background and aims

The aim of this study was to determine Zn/Cd accumulation ability and cellular distribution in Sedum plumbizincicola from a mine area.

Methods

Spatial localization of S, Cl, K, Ca, Ti, Mn, Fe, Ni, Cu, Zn and Cd in frozen-hydrated sections of S. plumbizincicola was quantitatively determined using cryo-micro-PIXE.

Results

The shoots of S. plumbizincicola hyperaccumulated up to 1,470 mg kg−1 of Cd and 14,600 mg kg−1 of Zn (dry weight) in field-grown plants. Micro-PIXE analyse shows that in roots Zn was concentrated in the cortex. In stems Zn was preferentially accumulated in the epidermis (5,090 mg kg−1 wet weight) and in vascular bundles and neighbouring parenchyma cells. Interestingly, some vascular bundles of the stem were depleted in Zn. Taking tissue area into account, 22.1, 46.2 and 31.8 % of total Zn and 17.1, 71.6 and 11.3 % of total Cd in the stem were present in the epidermis, cortex and central cylinder, respectively. In the leaves, 81.3 % of total Cd and 55.2 % of total Zn were distributed in the mesophyll.

Conclusions

The parenchyma cells, e.g. cortex in stem and mesophyll in leaf, play more important roles in Cd storage and detoxification than Zn in S. plumbizincicola.

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (Projects 41325003 and 41201300), the National High-technology R&D Program (863 Program) of China (Project 2012AA06A204) and the South African National Research Foundation. We thank Dr Dirk Frei and Ms Riana Rossouw of Stellenbosch University for their assistance in LA-ICP-MS measurements.

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Authors and Affiliations

  1. Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China

    Pengjie Hu, Zhu Li & Longhua Wu

  2. iThemba LABS, National Research Foundation, PO Box 722, Somerset West, 7129, South Africa

    Yaodong Wang, Wojciech Józef Przybyłowicz, Alban Barnabas & Jolanta Mesjasz-Przybyłowicz

  3. Faculty of Physics & Applied Computer Science, AGH University of Science and Technology, Al. A. Mickiewicza 30, 30-059, Krakow, Poland

    Wojciech Józef Przybyłowicz

  4. Yantai Institute of Coastal Zone Research, Yantai, 264003, China

    Yongming Luo

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  1. Pengjie Hu
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  2. Yaodong Wang
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Correspondence to Wojciech Józef Przybyłowicz or Longhua Wu.

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Responsible Editor: Henk Schat.

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Hu, P., Wang, Y., Przybyłowicz, W.J. et al. Elemental distribution by cryo-micro-PIXE in the zinc and cadmium hyperaccumulator Sedum plumbizincicola grown naturally. Plant Soil 388, 267–282 (2015). https://doi.org/10.1007/s11104-014-2321-4

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