Abstract
In this study, we demonstrate the selective in vivo detection of diadinoxanthin (DD) and diatoxanthin (DT) in intact Cyclotella cells using resonance Raman spectroscopy. In these cells, we were able to assess both the content of DD and DT carotenoids relative to chlorophyll and their conformation. In addition, the sensitivity and selectivity of the technique allow us to discriminate between different pools of DD on a structural basis, and to follow their fate as a function of the illumination conditions. We report that the additional DD observed when cells are grown in high-light conditions adopts a more twisted conformation than the lower levels of DD present when the cells are grown in low-light (LL) conditions. Thus, we conclude that this pool of DD is more tightly bound to a protein-binding site, which must differ from the one occupied by the DD present in LL conditions.
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Acknowledgments
This study is funded by FOM-program The Thylakoid Membrane: A Dynamic Switch (FP126; 12.0344) and the Fondation de l’Ecole Normale Superieure (MTAA and RvG) and the Advanced Investigator Grant (267333, PHOTPROT) from the European Research Council (ERC) (R.v.G. & BR). CB gratefully acknowledges the support from the Deutsche Forschungsgemeinschaft (DFG grants Bu 812-4/5).
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Alexandre, M.T.A., Gundermann, K., Pascal, A.A. et al. Probing the carotenoid content of intact Cyclotella cells by resonance Raman spectroscopy. Photosynth Res 119, 273–281 (2014). https://doi.org/10.1007/s11120-013-9942-y
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DOI: https://doi.org/10.1007/s11120-013-9942-y