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Ascorbic acid and xylem development in trunks of the Siberian larch trees

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Abstract

The contents of ascorbic acid (AA) and its oxidized form, dehydroascorbic acid (DHA), were assessed as related to the tracheid differentiation in the course of early and late wood development in the Siberian larch (Larix sibirica Ldb.) trees. The samples of the cambium, cell enlargement zone and mature cells were collected at the successive developmental stages by scraping tissues off layer by layer from trunk segments of the 20-year-old trees according to anatomical and histochemical criteria. While cambium initials were rapidly dividing, the AA contents per dry weight and per cell considerably exceeded the corresponding values characteristic of the late xylem development; such difference corresponded to the higher number of early tracheids per annual ring, as compared to the late tracheids. The AA content decreased as cells enlarged. The radial growth of the early wood tracheids, as compared to the late wood tracheids, was accompanied with a threefold increase in the AA and a decline in the DHA contents. The AA/DHA ratio was in line with the early tracheid enlargement. The maximum AA content was observed at the early stage of the secondary cell wall thickening in the tracheids of early and late xylem preceding lignification. During this stage of early wood development, the DHA content exceeded sixfold the corresponding value in the late xylem; as a result, the initial rates of lignification were different in two tissues. The rate of lignification in a newly developing layer of the early xylem increased gradually and was the highest in the completely differentiated tracheids. In the late xylem, the lignification rate was at its highest at the very beginning and then declined in the course of tracheid maturation. The dissimilar patterns of lignification in the early and late xylem were primarily associated with the DHA content, which increased in the early xylem and decreased in the maturing late xylem. Thus, the AA content and its accessibility to oxidation in the growing and mature xylem cells exhibited the diverse developmental patterns in the early and late xylem: two tissues differed in the tracheid number and radial diameter as well as in the rate of lignification.

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Abbreviations

AA:

ascorbic acid

CEZ:

cell enlargement zone

CZ:

cambium zone

DHA:

dehydroascorbic acid

MX:

mature xylem

MZ:

maturation zone (zone of the secondary cell wall deposition and lignification)

REFERENCES

  1. O. Arrigoni (1994) ArticleTitleAscorbate System and Plant Development J. Bioenerg. Biomembr. 26 407–419

    Google Scholar 

  2. A.A.F. Rautenkranz L. Li F. Machler E. Martinoia J. Oertli (1994) ArticleTitleTransport of Ascorbic and Dehydroascorbic Acids across Protoplast and Vacuole Membranes Isolated from Barley Plant Physiol. 106 187–193

    Google Scholar 

  3. R. Liso A.M. Innocenti B.M. Bitonti O. Arrigoni (1988) ArticleTitleAscorbic Acid-Induced Progression of Quiescent Centre Cells from G1 to S Phase New Phytol. 110 469–471

    Google Scholar 

  4. M. Sánchez E. Quejieiro G. Revilla I. Zarra (1997) ArticleTitleChanges in Ascorbic Acid Levels in Apoplastic Fluid during Growth of Pine Hypocotyls: Effect on Peroxidase Activity Associated with Cell Walls Physiol. Plant. 101 815–820

    Google Scholar 

  5. I. Zarra M. Sánchez E. Quejieiro M.J. Reña G. Revilla (1999) ArticleTitleThe Cell Wall Stiffening Mechanism in Pinus pinaster Aiton: Regulation by Apoplastic Levels of Ascorbate and Hydrogen Peroxide J. Sci. Food Agric. 79 416–420

    Google Scholar 

  6. U. Takahama (1993) ArticleTitleRegulation of Peroxidase-Dependent Oxidation of Phenolics by Ascorbic Acid: Different Effects of Ascorbic Acid on the Oxidation of Coniferyl Alcohol by the Apoplastic Soluble and Cell Wall-Bound Peroxidases from Epicotyls of Vigna angularis Plant Cell Physiol. 34 809–817

    Google Scholar 

  7. U. Takahama (1994) ArticleTitleChanges Induced by Abscisic Acid and Light in the Redox State of Ascorbate in the Apoplast of Epicotyls of Vigna angularis Plant Cell Physiol. 35 975–978

    Google Scholar 

  8. R. Zahner (1963) ArticleTitleInternal Moisture Stress and Wood Formation in Conifers Forest Prod. 13 240–247

    Google Scholar 

  9. G.F. Antonova (1999) Rost kletok khvoinykh Nauka Novosibirsk

    Google Scholar 

  10. Antonova, G.F. and Shebeko, V.V., Usage of Cresyl Violet for Studying of Wood Formation, Khimiya drevesiny, 1981, no. 4, pp. 102–105.

  11. V.E. Moskaleva (1958) ArticleTitleFormation of Pine Tracheids Tr. In-ta lesa Akad. Nauk SSSR 37 254–265

    Google Scholar 

  12. Odintsov, P.P., Katkevich, P.G., Pendere, M.K., and Katkevich, Yu.Yu., Uronic Acids in Young Spruce Shoots, Izv. Akad. Nauk LatvSSR, Ser. Chem., 1967, no. 3, pp. 632–639.

  13. M. Dubois R.A. Gilles J.K. Hamilton P.A. Rebers F. Smith (1956) ArticleTitleColorimetric Method for Determination of Sugars and Related Substances Anal. Chem. 28 350–356

    Google Scholar 

  14. J.H. Roe M.J. Oesterling (1944) ArticleTitleThe Determination of Dehydroascorbic Acid and Ascorbic Acid in Plant Tissues by the 2,4-Dinitrophenylhydrazine Method J. Biol. Chem. 152 511–517

    Google Scholar 

  15. Sokolovskii, V.V., Lebedeva, L.V., and Lielup, T.B., Method for Separate Determination of Dehydroascorbic and Diketogulonic Acids in Biological Tissues, Lab. delo, 1974, no. 3, pp. 160–162.

  16. I.A. Chaplygina G.F. Antonova (2002) The Determination of Ascorbic and Dehydroascorbic Acids during Wood Formation in Larix sibirica Botanicheskie issledovaniyav Sibiri Krasnoyarsk. Otd. Bot. O-va Ross. Akad. Nauk Krasnoyarsk 254–257

    Google Scholar 

  17. C.J. Venverloo (1969) ArticleTitleThe Lignin of Populus nigra L., cv. Italica: A Comparative Study of the Lignified Structures in Tissue Cultures and the Tissue of the Trees Acta Bot. Neerl. 18 241–314

    Google Scholar 

  18. N. Smirnoff S.V. Colombe (1988) ArticleTitleDrought Influence on the Activity of Enzymes of Chloroplast Hydrogen Peroxide Scavenging System J. Exp. Bot. 39 1097–1108

    Google Scholar 

  19. S.P. Mukherjee M.A. Choudhuri (1983) ArticleTitleImplication of Water Stress-Induced Changes in the Levels of Endogenous Ascorbic Acid and Hydrogen Peroxide in Vigna Seedlings Physiol. Plant. 58 166–170

    Google Scholar 

  20. M.A. Hossain Y. Nakano K. Asada (1984) ArticleTitleMonodehydroascorbate Reductase in Spinach Chloroplasts and Its Participation in Regeneration of Ascorbate for Scavenging Hydrogen Peroxide Plant Cell Physiol. 25 385–395

    Google Scholar 

  21. D. Cosgrove (1997) ArticleTitleAssembly and Enlargement of the Primary Cell Wall in Plants Annu. Rev. Cell Dev. Biol. 9 1031–1041

    Google Scholar 

  22. M. Sánchez M.J. Peña G. Revilla I. Zarra (1996) ArticleTitleChanges in Dehydrodiferulic Acid and Peroxidase Activity against Ferulic Acid Associated with Cell Walls during Growth of Pinus pinaster Hypocotyl Plant Physiol. 111 941–946

    Google Scholar 

  23. W. Bao D.M. O’Malley R. Whetten R.R. Sederoff (1993) ArticleTitleA Laccase Associated with Lignification in Loblolly Pine Xylem Science 260 672–674

    Google Scholar 

  24. G.J. McDougall D. Stewart I.M. Morrison (1994) ArticleTitleCell-Wall-Bound Oxidases from Tobacco (Nicotiana tabacum) Xylem Participate in Lignin Formation Planta 194 9–14

    Google Scholar 

  25. R. Sterjiades J.F.D. Dean G. Gamble D.S. Himmelsbach K.-E.L. Eriksson (1993) ArticleTitleExtracellular Laccases and Peroxidases from Sycamore Maple (Acer pseudoplatanus) Cell-Suspension Cultures Planta 190 75–87

    Google Scholar 

  26. R. Savidge P. Udagama-Randeniya (1992) ArticleTitleCell Wall-Bound Coniferyl Alcohol Oxidase Associated with Lignification in Conifers Phytochemistry 31 2959–2966

    Google Scholar 

  27. M.A. Hossain Y. Nakano K. Asada (1984) ArticleTitleMonodehydroascorbate Reductase in Spinach Chloroplasts and Its Participation in Regeneration of Ascorbate for Scavenging Hydrogen Peroxide Plant Cell Physiol. 25 385–395

    Google Scholar 

  28. G. Forti G. Elli (1995) ArticleTitleThe Function of Ascorbic Acid in Photosynthetic Phosphorylation Plant Physiol. 109 1207–1211

    Google Scholar 

  29. C. Paciolla M. Tullio Particlede A. Chiappetta A.M. Innocenti V.B. Bitonti R. Liso O. Arrigoni (2001) ArticleTitleShort-and Long-Term Effects of Dehydroascorbate in Lupinus albus and Allium cepa Roots Plant Cell Physiol. 42 857–863

    Google Scholar 

  30. U. Takahama T. Oniki (1994) ArticleTitleThe Association of Ascorbate and Ascorbate Oxidase in the Apoplast with IAA-Enhanced Elongation of Epicotyls from Vigna angularis Plant Cell Physiol. 35 257–266

    Google Scholar 

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Authors and Affiliations

  1. Sukachev Institute of Forestry, Siberian Division, Russian Academy of Sciences, Akademgorodok, 660036, Krasnoyarsk, Russia

    G. F. Antonova, I. A. Chaplygina, T. N. Varaksina & V. V. Stasova

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  1. G. F. Antonova
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  2. I. A. Chaplygina
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  3. T. N. Varaksina
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  4. V. V. Stasova
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Translated from Fiziologiya Rastenii, Vol. 52, No. 1, 2005, pp. 97–107.

Original Russian Text Copyright © 2005 by Antonova, Chaplygina, Varaksina, Stasova.

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Antonova, G.F., Chaplygina, I.A., Varaksina, T.N. et al. Ascorbic acid and xylem development in trunks of the Siberian larch trees. Russ J Plant Physiol 52, 83–92 (2005). https://doi.org/10.1007/s11183-005-0013-x

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  • DOI: https://doi.org/10.1007/s11183-005-0013-x

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