Skip to main content
SpringerLink
Log in

Impact of 9 years of Bt-maize cultivation on the distribution of maize viruses

  • Original Paper
  • Published:
Transgenic Research Aims and scope Submit manuscript

Abstract

This study assesses the effect of Bt-maize on the distribution of maize viruses. Random surveys were conducted in Spain between 2001 and 2006 to evaluate the occurrence of maize viruses in Bt-maize cultivation areas and in areas where this crop had not been introduced. Maize dwarf mosaic virus (MDMV) was the predominant virus in Bt-areas, and Maize rough dwarf virus (MRDV) was the most predominant one in non-Bt-areas, with MRDV an emergent virus in both types of areas. A decline in the occurrence of MDMV and an increase in that of Sugarcane mosaic virus was observed in Bt-areas. Additionally, data obtained over 6 years in experimental fields showed non-significant differences between the infection rates exhibited by two generations of Bt varieties and the non-transformed isogenics varieties for any of the viruses. Our data suggest that differences in virus distribution are linked to the genetic background of the maize varieties and the distribution of virus reservoirs rather than to Bt-maize cultivation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Achon MA (1999) Incidence of maize dwarf mosaic and sugarcane mosaic virus in Northeast of Spain. Z Pflanzenk Pflanzen—J Plant Dis Prot 105:539–544

    Google Scholar 

  • Achon MA, Sobrepere M (2001) Incidence of potyviruses in commercial maize fields and their seasonal cycles in Spain. Z Pflanzenk Pflanzen—J Plant Dis Prot 108:399–406

    CAS  Google Scholar 

  • Achon MA, Lomonossoff GP, Medina V (1995) Studies on maize dwarf mosaic virus (MDMV) in northeast Spain. Agronomie 15:501. doi:10.1051/agro:19950733

    Article  Google Scholar 

  • Achon MA, Pinner M, Medina M, Lomonossoff GP (1996) Biological characteristics of maize dwarf mosaic Potyvirus from Spain. Eur J Plant Pathol 102:697–705. doi:10.1007/BF01877251

    Article  Google Scholar 

  • Achon MA, Sobrepere M, Minguell R (2003) Molecular and biological properties of a sugarcane mosaic Potyvirus isolate from Spain. Z Pflanzenk Pflanzen—J Plant Dis Prot 110:324–331

    CAS  Google Scholar 

  • Achon MA, Serrano L, Alonso-Dueñas N, Porta C (2007) Complete genome sequences of maize dwarf mosaic and sugarcane mosaic virus isolates coinfecting maize in Spain. Arch Virol 152:2073–2978. doi:10.1007/s00705-007-1042-x

    Article  PubMed  CAS  Google Scholar 

  • Castañera P (1986) Plagas del maíz. In: Proc 4th Jornadas Técnicas Maíz Plagas. Lleida, pp 1–24

  • Clark MF, Adams AN (1977) Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J Gen Virol 34:475–483. doi:10.1099/0022-1317-34-3-475

    Article  PubMed  CAS  Google Scholar 

  • DePaulo JJ, Powell CA (1995) Extraction of double-stranded-RNA from plant-tissues without the use of organic-solvents. Plant Dis 79:246–248

    CAS  Google Scholar 

  • Eizaguirre M, Albajes R, Lopez C, Eras J, Lumbierres B, Pons X (2006) Six years after the commercial introduction of Bt maize in Spain: field evaluation, impact and future prospects. Transgenic Res 15:1–12. doi:10.1007/s11248-005-3998-1

    Article  PubMed  CAS  Google Scholar 

  • Fearing PL, Brown D, Viachos D, Meghji M, Privalle L (1997) Quantitative analysisi of CryIA (b) expression in Bt maize plants, tissues, and silage and stablility of expression over successive generations. Mol Breed 3:167–176. doi:10.1023/A:1009611613475

    Article  Google Scholar 

  • Fuchs E, Gruntzig M, Hohmann F, Kuntze L, Oertel U (1997) 15 years research work on virus diseases of maize in central Germany. Kuhn-Arch 91:3–34

    Google Scholar 

  • Grilli MP, Bruno M (2007) Regional abundance of a planthopper pest: the effect of host patch area and configuration. Entomol Exp Appl 122:133–143. doi:10.1111/j.1570-7458.2006.00498.x

    Article  Google Scholar 

  • Grilli MP, Gorla DE (1998) The effect of agroecosystem management on the abundance of Delphacodes kuscheli (Homoptera: Delphacidae), vector of the maize rough dwarf virus, in central Argentina. Maydica 43:77–82

    Google Scholar 

  • Hohmann F, Fuchs E, Gruntzig M, Oertel U (1999) A contribution to the ecology of sugarcane mosaic Potyvirus (SCMV) and maize dwarf mosaic Potyvirus (MDMV) in Germany. Z Pflanzenk Pflanzen—J Plant Dis Prot 106:314–324

    Google Scholar 

  • Icoz I, Stotzky G (2008) Fate and effects of insect-resistant Bt crops in soil ecosystems. Soil Biol Biochem 40:559–586. doi:10.1016/j.soilbio.2007.11.002

    Article  CAS  Google Scholar 

  • Ilbagi H, Rabenstein F, Habekuss A, Ordon F, Citir A (2006) Incidence of virus diseases in maize fields in the Trakya region of Turkey. Phytoprotection 87:115–122

    CAS  Google Scholar 

  • Lenardon SL (1987) Mal de Rio Cuarto. Revista Crea, pp. 6–15

  • Lenardon SL, March GJ, Nome SF, Ornaghi JA (1998) Recent outbreak of “Mal de Rio Cuarto” virus on corn in Argentina. Plant Dis 82:448. doi:10.1094/PDIS.1998.82.4.448C

    Article  Google Scholar 

  • Lin CS, Poushinsky G, Mauer M (1979) Examination of 5 sampling methods under random and clustered disease distributions using simulation. Can J Plant Sci 59:121–130

    Article  Google Scholar 

  • Lindsten K, Gerhardson B, Petterson J (1973) Cereal tillering disease in Sweden and some comparisons with oat sterile dwarf and maize rough dwarf. Medd fran Statens vaxtskyddsanst 15:375–379

    Google Scholar 

  • Lovisolo O (1971) Maize rough dwarf virus. CMI/AAB descriptions of plant viruses no. 72. Commonwealth Mycological Institute, Kew, Surrey

  • Milne RG, Lovisolo O (1977) Maize rough dwarf and related viruses. Adv Virus Res 21:267–341. doi:10.1016/S0065-3527(08)60764-2

    Article  PubMed  CAS  Google Scholar 

  • Pokorny R, Porubova M (2000) The occurrence of viral pathogens of the genus Potyvirus on maize (Zea mays L.) in the Czech Republic. Z Pflanzenk Pflanzen—J Plant Dis Prot 107:329–336

    Google Scholar 

  • Pons X, Lumbierres B, Lopez C, Albajes R (2005) Abundance of non-target pest in transgenic Bt-maize: a farm scale study. Eur J Entomol 102:73–79

    CAS  Google Scholar 

  • Recasens J, Riba F, Izquierdo J, Forn R, Taberner A (1996) Gramíneas infestantes de los cereales de invierno de Cataluña. ITEA 92:116–130

    Google Scholar 

  • Rodríguez Pardina PE, Gimenez Pecci MP, Laguna IG, Dagoberto E, Truol G (1998) Wheat: a new natural host for the Mal de Rio Cuarto virus in the endemic disease area, Rio Cuarto, Cordoba Province, Argentina. Plant Dis 82:149–152. doi:10.1094/PDIS.1998.82.2.149

    Article  Google Scholar 

  • Romeis J, Meissle M, Bigler F (2006) Transgenic crops expressing Bacillus thuringiensis toxins and biological control. Nat Biotechnol 24:63–71. doi:10.1038/nbt1180

    Article  PubMed  CAS  Google Scholar 

  • SAS Institute (2003) SAS/STAT user’s guide, Version 8. SAS Institute, Cary

    Google Scholar 

  • Signoret PA, Alliot B (1995) Biological and serological diversity of a Potyvirus infecting maize in the Mediterranean area. Agronomie 15:439–441. doi:10.1051/agro:19950709

    Article  Google Scholar 

  • Sisquella M, Lloveras J, Alvaro J, Santiveri P, Cantero C (2004) Técnicas de cultivo para la producción de maíz, trigo y alfalfa en regadíos del valle del Ebro. Proyecto Trama. Fundació Catalana de Cooperació, Lleida

    Google Scholar 

  • Sobrepere M, Achon MA (2001) Emergence of maize rough dwarf Fijivirus in Spain. In: 9th Conf Virus Dis Gramineae Europe. York, UK

Download references

Acknowledgments

This study was supported by projects AGF1999–0782 and AGL2003–04077 of Spanish Ministry of Science and Innovation. We are grateful to Dr. L. Serrano for helpful statistical analysis and Dr. C. Porta for helpful discussions.

Author information

Authors and Affiliations

  1. Departamento de Producción Vegetal y Ciencia Forestal, Universitat de Lleida, Rovira Roure 191, 25198, Lleida, Spain

    Maria Angeles Achon & Natalia Alonso-Dueñas

Authors
  1. Maria Angeles Achon
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Natalia Alonso-Dueñas
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Maria Angeles Achon.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Achon, M.A., Alonso-Dueñas, N. Impact of 9 years of Bt-maize cultivation on the distribution of maize viruses. Transgenic Res 18, 387–397 (2009). https://doi.org/10.1007/s11248-008-9231-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11248-008-9231-2

Keywords

Search

Navigation