Abstract
To obtain basic information for evaluating critical levels of O3 under different nitrogen loads for protecting Japanese beech forests, two-year-old seedlings of Fagus crenata Blume were grown in potted andisol supplied with N as NH4NO3 solution at 0, 20 or 50 kg ha−1 year−1 and exposed to charcoal-filtered air or O3 at 1.0, 1.5 and 2.0 times the ambient concentration from 16 April to 22 September 2004. The O3 induced significant reductions in the whole-plant dry mass, net photosynthetic rate at 380 μmol mol−1 CO2 (A 380), carboxylation efficiency (CE) and concentrations of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and total soluble protein (TSP) in the leaves. The concentrations of Rubisco and TSP were negatively correlated with the concentration of leaf acidic amino acid, suggesting that O3 enhanced the degradation of protein such as Rubisco. The N supply to the soil did not significantly change the whole-plant dry mass and A 380, whereas it significantly increased the CE and concentrations of Rubisco and total amino acid. No significant interactive effects of O3 and N supply to the soil were detected on the growth, photosynthetic parameters and concentrations of protein and amino acid in the leaves. In conclusion, N supply to the soil at ≤50 kg ha−1 year−1 does not significantly change the sensitivity to O3 of growth and net photosynthesis of Fagus crenata seedlings.
Similar content being viewed by others
References
Brendley, B. W., & Pell, E. J. (1998). Ozone-induced changes in biosynthesis of Rubisco and associated compensation to stress in foliage of hybrid poplar. Tree Physiology, 18, 81–90.
Bytnerowicz, A., Godzik, B., Grodzinska, K., Fraczek, W., Musselman, R., Manning, W., et al. (2004). Ambient ozone in forests of the central and eastern European mountains. Environmental Pollution, 130, 5–16.
Chappelka, A. H., & Samuelson, L. J. (1998). Ambient ozone effects on forest trees of the eastern United States: A review. New Phytologist, 139, 91–108.
Delrot, S., Rochat, C., Tegeder, M., & Frommer, W. B. (2001). Plant nitrogen. Paris: INRA-Springer, pp. 215.
Derwent, R., Collins, W., Johnson, C., & Stevenson, D. (2002). Global ozone concentrations and regional air quality. Environmental Science & Technology, 36, 379–382.
Izuta, T., Yamaoka, T., Nakaji, T., Yonekura, T., Yokoyama, M., Funada, R., et al. (2004). Growth, net photosynthesis and leaf nutrient status of Fagus crenata seedlings grown in brown forest soil acidified with H2SO4 or HNO3 solution. Trees, 18, 677–685.
Maruta, E., Shima, K., Horie, K., Aoki, M., Dokiya, Y., Izuta, T., et al. (1999). Forest decline of Fagus crenata at Mt. Hinokiboramaru (Tanzawa, Kanagawa Prefecture, Japan) and acid deposition (in Japanese). Environmental Sciences (Japan), 12, 241–250.
Ministry of the Environment (2004). Summary report of research results under the GERF (Global Environment Research Fund) in FY2003. Japan: Ministry of the Environment, pp. 238.
Nakaji, T., Fukami, M., Dokiya, Y., & Izuta, T. (2001). Effects of high nitrogen load on growth, photosynthesis and nutrient status of Cryptomeria japonica and Pinus densiflora seedlings. Trees, 15, 453–461.
Nihlgård, B. (1985). The ammonium hypothesis – an additional explanation to the forest dieback in Europe. Ambio 14, 2–8.
Okochi, H., & Igawa, M. (2001). Elevational patterns of acid deposition into a forest and nitrogen saturation on Mt. Oyama, Japan. Water, Air, and Soil Pollution, 130, 1091–1096.
Pääkkönen, E., & Holopainen, T. (1995). Influence of nitrogen supply on the response of clones of birch (Betula pendula Roth.) to ozone. New Phytologist, 129, 595–603.
Pell, E. J., Sinn, J. P., & Johansen, V. (1995). Nitrogen supply as a limiting factor determining the sensitivity of Populus tremuloides Michx. to ozone stress. New Phytologist, 130, 437–446.
Vingarzan, R. (2004). A review of surface ozone background levels and trends. Atmospheric Environment, 38, 3431–3442.
Wright, R. F., Brandrud, T.-E., Clemensson-Lindell, A., Hultberg, H., Kjønaas, O. J., Moldan, F., et al. (1995). NITREX Project: Ecosystem response to chronic additions of nitrogen to a spruce-forested catchment at Gårdsjön, Sweden. Ecological Bulletins, 44, 322–334.
Yonekura, T., Honda, Y., Oksanen, E., Yoshidome, M., Watanabe, M., Funada, R., et al. (2001). The influences of ozone and soil water stress, singly and in combination, on leaf gas exchange rates, leaf ultrastructural characteristics and annual ring width of Fagus crenata seedlings. Journal of Japan Society for Atmospheric Environment, 36, 333–352.
Acknowledgements
This research was funded by a grant from Ministry of the Environment, Japan (Global Environment Research Fund). The authors are greatly indebted to Prof. Hisao Itabashi and Prof. Keiji Hasumi of Tokyo University of Agriculture and Technology for their invaluable advice on the analysis of amino acid.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Yamaguchi, M., Watanabe, M., Matsuo, N. et al. Effects of Nitrogen Supply on the Sensitivity to O3 of Growth and Photosynthesis of Japanese Beech (Fagus crenata) Seedlings. Water Air Soil Pollut: Focus 7, 131–136 (2007). https://doi.org/10.1007/s11267-006-9094-6
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11267-006-9094-6