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Microbial Indicators of Faecal Contamination in Water: A Current Perspective

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Abstract

It is well documented that faecal contamination of drinking water has caused numerous disease outbreaks. Because the risks of disease outbreaks correlate with the incidence of faecal contamination, faecal bacteria are used as indicators of faecal contamination and hence, the possible presence of disease-causing organisms. However, different microbiological faecal indicators are used in different countries and jurisdictions. Therefore, it is important to understand the potentials and limitations of these indicator organisms before realistically implementing guidelines and regulations to safeguard our water resources. This review considers the history of indicator organisms, the evolution of the analytical methodologies (biochemical and molecular) and addresses the advantages and limitations of current faecal indicator microorganisms.

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References

  • Allen, M. J. and Edberg, S. C.: 1995, ‘The {public health significance of bacterial indicators} in Drinking Water’, The Royal Society of Chemistry 1999, Special Publication. Atheneaum Press UK.

  • Alonso, J. L., Soriano, A., Carbajo, O., Amoros, I. and Garelick, H.: 1999, ‘Comparison and {recovery of} Escherichia coli and {thermotolerant coliforms in water with a chromogenic medium incubated at} 41 and 44.5 C’, Appl. Environ. Microbiol. 65, 3746–3749.

    PubMed  Google Scholar 

  • American Public Health Association: 1992, Standard Methods for the Examination of Water and Wastewater, 19th ed, American Public Health Association, Washington, D.C.

  • American Public Health Association: 1998, Standard Methods for the Examination of Water and Wastewater, 20th ed, American Public Health Association, Washington, D.C.

  • Archibald, F.: 2000, ‘The presence of coliform bacteria in Canadian pulp and paper mill water systems~– a cause for concern?’, Water Qual. Res. J. Canada 35, 1–22.

    Google Scholar 

  • Ashbolt, N. J., Grabow, W. O. and Snozzi, M.: 2001, ‘Indicators of {microbial water quality}’, in L. Fewtrell and J. Bartram (eds), Water Quality – Guidelines, Standards and Health: Assessment of Risk and Risk Management for Water-Related Infectious Disease, World Health Organization, Geneva, Switzerland.

    Google Scholar 

  • Baggi, F., Demarta, A. and Peduzzi, R.: 2001, ‘Persistence of viral pathogens and bacteriophages during sewage treatment: Lack of correlation with indicator bacteria’, Res. Microbiol. 152(8), 743–751.

    Article  PubMed  Google Scholar 

  • Baudart, J., Coallier, J., Laurent, P. and Prévost, M.: 2002, ‘Rapid and {sensitive enumeration of viable diluted cells of members of the family} Enterobacteriaceae in {freshwater and drinking water}’, Appl. Environ. Microbiol. 68, 5057–5063.

    Article  PubMed  Google Scholar 

  • Baudizšová, D.: 1997, ‘Evaluation of Escherichia coli as the {main indicator of faecal pollution}’, Wat. Sci. Tech. 35, 333–336.

    Article  Google Scholar 

  • Bej, A. K., McCarty, S. C. and Atlas, R. M.: 1991, ‘Detection of coliform bacteria and Escherichia coli by multiplex polymerase chain reaction: Comparison with defined substrate and plating methods for water quality monitoring’, Appl. Environ. Microbiol. 57, 2429–2432.

    PubMed  Google Scholar 

  • Bej, A. K., Steffan, R. J., DiCesare, J., Haff, L. and Atlas, R. M.: 1990, ‘Detection of coliform bacteria in water by polymerase chain reaction and gene probes’, Appl. Environ. Microbiol. 56, 307– 314.

    PubMed  Google Scholar 

  • Byamukama, D, Kansiime, F., Mach, R. L. and Farnleitner, L. H.: 2000, ‘Determination of Escherichia coli contamination with {chromocult coliform agar showed a high level of discrimination efficiency for differing fecal pollution levels in tropical waters of} Kampala, Uganda’, Appl. Environ. Microbiol. 66, 864–868.

    Article  PubMed  Google Scholar 

  • Camper, A. K., McFeters, G. A., Characklis, W. G. and Jones, W. L.: 1991, ‘Growth kinetics of coliform bacteria under conditions relevant to drinking-water distribution systems’, Appl. Environ. Microbiol. 57, 2233–39.

    PubMed  Google Scholar 

  • Carrillo, M., Estrada, E. and Hazen, T. C.: 1985, ‘Survival and enumeration of the fecal indicators Bifidobacterium adolescentis and Escherichia coli in tropical rain forest watershed’, Appl. Environ. Microbiol. 50, 468–476.

    PubMed  Google Scholar 

  • Carson, C. A., Shear, B. L., Ellersieck, M. R. and Asfaw, A.: 2001, ‘Identification of fecal Escherichia coli from humans and animals by ribotyping’, Appl. Environ. Microbiol. 67, 1503–1507.

    Article  PubMed  Google Scholar 

  • Chang, H. T., Rittmann, B. E., Amar, D., Heim, R., Ehlinger, O. and Lesty, Y.: 1991, ‘Biofilm detachment mechanisms in a liquid-fluidized bed’, Biotechnol. Bioeng. 38, 499–506.

    Article  Google Scholar 

  • Chang, H. T. and Rittmann, B. E.: 1988, ‘Comparative study of biofilm shear loss on different adsorptive media’, J. Water Pollut. Control Fed. 60, 362–368.

    Google Scholar 

  • Charoenca, N. and Fujioka, R. S.: 1991, ‘Assessment of Staphylococcus bacteria in Hawaii’s marine recreational waters’, Water Sci. Technol. 27, 283–289.

    Google Scholar 

  • Ciebin, B. W., Brodsky, M. H., Eddiington, R., Horsnell, G., Choney, A., Palmateer, G., Ley, A., Joshi, R. and Shears, G.: 1995, ‘Comparative {evaluation of modified} m-FC and m-TEC {media for membrane filter enumeration of} Escherichia coli in water’, Appl. Environ. Microbiol. 61, 3940–3942.

    PubMed  Google Scholar 

  • Colbourne, J. S.: 1985, ‘Materials usage and their effects on the microbiological quality of water supplies’, J. Appl. Bacteriol. 59, 47–60.

    Google Scholar 

  • Conboy, M. J. and Goss, M. J.: 2001, ‘Identification of an {assemblage of indicator organisms to assess timing and source of bacterial contamination in groundwater}’, Water Air and Soil Pollution 129, 101–118.

    Article  Google Scholar 

  • Craun, G. F.: 1986, ‘Waterborne giardiasis in the United States 1965–1984’, Lancet 2, 513–514.

    Article  Google Scholar 

  • Delabre, K., Dile, V., De Roubin, M. R., Gatel, D., Poty, F. and Cavard, J.: 2001, ‘New analytical tools for distribution system surveillance’, Proceedings of AWWA-Annual Conference, American Water Works Association, Washington, DC.

  • Dick, L. K. and Field, K. G.: 2003, ‘(in review) A quantitative PCR assay for Bacteroidetes 16S rDNA provides a rapid estimate of fecal pollution in water’, Appl. Environ. Microbiol.

  • Dogan-Halkman, H. B., Çakir, İ., Keven, F., Worobo, R. and Halkman, A. K.: 2003, ‘Relationship among fecal coliforms and Escherichia coli in various food’, European Food Research and Technology 216(4), 331–334.

    Google Scholar 

  • Dombek, P. E., Johnson, L. K., Zimmerley, S. T. and Sadowsky, M. J.: 2000, ‘Use of repetitive DNA sequences and the PCR to differentiate Escherichia coli isolates from human and animal sources’, Appl. Environ. Microbiol. 66, 2572–2577.

    Article  PubMed  Google Scholar 

  • Doolittle, M. M., Cooney, J. J. and Caldwell, D. E.: 1996, ‘Tracing the interaction of bacteriophage with bacterial biofilms using fluorescent and chromogenic probes’, J. Ind. Microbiol. 16, 331–341.

    Article  PubMed  Google Scholar 

  • Dufour, P.: 1977, Escherichia coli: The Fecal Coliform. Spec. Tech. Publ. 65, pp. 48–58. Philadelphia: Am. Soc. Test. Mater.

  • Dukan, S., Levi, Y., Piriou, P., Guyon F. and Villon P.: 1996, ‘Dynamic modelling of bacterial growth in drinking-water networks’, Water Res. 30(9), 1991–2002.

    Article  Google Scholar 

  • DuPont, H. L., Chappell, C. L., Sterling, C. R., Okhuysen, P. C., Rose, J. B. and Jakubowski, W.: 1995, ‘Infectivity of Cryptosporidium parvum for adult humans’, N. Engl. J. Med. 332, 855–859.

    Article  PubMed  Google Scholar 

  • Edberg, S. C., Allen, M. J., Smith, D. B. and Kriz, N. J.: 1990, ‘Enumeration of total coliforms and Escherichia coli from source water by the defined substrate technology’, Appl. Environ. Microbiol. 56, 366–369.

    PubMed  Google Scholar 

  • Edberg, S. C., Allen, M. J., Smith, D. B. and The National Collaborative Study: 1988, ‘National field evaluation of a defined substrate method for the simultaneous enumeration of total coliforms and Escherichia coli from drinking water: Comparison with the standard multiple tube fermentation method’, Appl. Environ. Microbiol. 54, 1595–1601.

    PubMed  Google Scholar 

  • Edberg, S. C., Leclerc, H. and Robertson, J.: 1997, ‘Natural protection of spring and well drinking water against surface microbial contamination. II. Indicators and monitoring parameters for parasites’, Crit. Rev. Microbiol. 23, 179–206.

    PubMed  Google Scholar 

  • Edberg, S. C., Rice, E. W., Karlin, R. J. and Allen, M. J.: 2000, ‘Escherichia coli: The best biological drinking water indicator for public health protection’, J. Appl. Microbiol. Suppl. 88, 106S–116S.

    Google Scholar 

  • Environmental Health Directorate – Health Protection Branch: 1978, Recreational Water Quality, Published by Authority of the Minister of National Health and Welfare.

  • European Economic Community: 1998, ‘Council Directive 98/83/EC relating to the quality of water intended for human consumption’, Off. J. Eur. Communities L330, 32–54.

    Google Scholar 

  • Fayer, R., Morgan, U. and Upton, S. J.: 2000, ‘Epidemiology of Cryptosporidium: transmission, detection and identification’, International Journal for Parasitology 30, 1305–1322.

    Article  PubMed  Google Scholar 

  • Federal-Provincial Working Group on Recreational Water Quality of the Federal-Provincial Advisory Committee on Environmental and Occupational Health: 1992, ‘Guidelines for Canadian Recreational Water Quality’, Minister of National Health and Welfare.

  • Federal-Provincial-Territorial Committee on Drinking Water: 2002, ‘Bacteriological Quality. Guidelines for Canadian Drinking Water Quality – Supporting Documents, Water Quality and Health Bureau of the Safe Environments Programme of Health Canada’, 2003-01–07.

  • Federal-Provincial-Territorial Committee on Drinking Water: April 2002, ‘Summary of Guidelines for Canadian Drinking Water Quality. Safe Environments Programme of Health Canada’.

  • Feng, P., Lum, R. and Chang, G. W.: 1991, ‘Identification of uidA gene sequences in β -D-glucuronidase-negative Escherichia coli’, Appl. Environ. Microbiol. 57, 320–323.

    PubMed  Google Scholar 

  • Feng, P. C. S. and Hartman, P. A.: 1982, ‘Fluorogenic assays for immediate confirmation of Escherichia coli’, Appl. Environ. Microbiol. 43, 1320–1329.

    PubMed  Google Scholar 

  • Finegold, S. M., Sutter, V. L. and Mathison, G. E.: 1983, ‘Normal indigenous intestinal flora’, in D. J. Hentges (ed), Human Intestinal Microflora in Health and Disease’, 1:3–31. New York: Academic.

  • Formiga-Cruz, M., Allard, A. K., Conden-Hansson, A. C., Henshilwood, K., Henroth, B. E., Jofre, J., Lees, D. N., Lucena, F., Papapetropoulou, M., Rangdale, R. E., Tsibouxi, A., Vantarakis, A. and Girones, R.: 2003, ‘Evaluation of {potential indicators of viral contamination in shellfish and their applicability to diverse geographical areas}’, Appl. Environ. Microbiol. 69, 1556– 1563.

    Article  PubMed  Google Scholar 

  • Fricker, E. J. and Fricker, C. R.: 1994, ‘Application of the polymerase chain reaction to the identification of Escherichia coli and coliforms in water’, Lett. Appl. Microbiol. 19, 44–46.

    PubMed  Google Scholar 

  • Fricker, E. J. and Fricker, C. R.: 1996, ‘Use of two presence/absence systems for the detection of E.~coli and coliforms from water’, Water Res. 30, 2226–2228.

    Article  Google Scholar 

  • Gauthier, F. and Archibald, F.: 2001, ‘The Ecology of “{faecal indicator” bacteria commonly found in pulp and paper mill water systems}’, Wat. Res. 35, 2207–2218.

    Article  Google Scholar 

  • Gavini, F., Leclerc, H. and Mossel, D. A. A.: 1985, ‘Enterobacteriaceae of the “coliform group” in drinking water: Identification and worldwide distribution’, Syst. Appl. Microbiol. 6, 312–318.

    Google Scholar 

  • Geldreich, E. E. and Kenner, B. A.: 1969, ‘Concepts of fecal streptococci in stream pollution’, J. Water Pollut. Control Fed. 41, R352–R355.

    Google Scholar 

  • Gerba, C. P. and Rose, J. B.: 1990, ‘Viruses in {source and drinking water}’, in G. A. McFeters (ed), Drinking-Water Microbiology: Progress and Recent Developments, New York: Springer-Verlag., pp. 380–396.

    Google Scholar 

  • Gleeson, C. and Gray, N.: 1997, The Coliform Index and Waterborne Disease, London: E {&} FN Spon. 194 pp.

  • Godfree, A. F., Kay, D. and Wyer, M. D.: 1997, ‘Faecal streptococci as indicators of faecal contamination in water’, Journal of Applied Microbiology Symposium Supplement 110S–119S.

  • Grabow, W. O., Taylor, M. B. and de Villiers, J. C.: 2001, ‘New {methods for the detection of viruses}: Call f{or review of drinking water quality guidelines}’, Water Sci Technol. 43(12), 1–8.

    Google Scholar 

  • Guan, S., Xu, R., Chen, S., Odumeru, J. and Gyles, C.: 2002, ‘Development of a procedure for discriminating among Escherichia coli isolates from animal and human sources’, Appl. Environ. Microbiol. 68, 2690–2698.

    Article  PubMed  Google Scholar 

  • Haas, C. N. and Rose, J. B.: 1994, ‘Reconciliation of microbial risk models and outbreak epidemiology: The case of the Milwaukee outbreak’, Proc. Water Works Assoc. pp. 517–23. Denver: Am. Water Works Assoc.

  • Hardalo, C. and Edberg, S. C.: 1997, ‘Pseudomonas aeruginosa: Assessment of risk from drinking water’, Crit. Rev. Microbiol. 23, 47–75.

    PubMed  Google Scholar 

  • Harwood, V. J., Whitlock, J. and Withington, V.: 2000, ‘Classification of antibiotic resistance patterns of indicator bacteria by discriminant analysis: Use in predicting the source of fecal contamination in subtropical waters’, Appl. Environ. Microbiol. 66, 3698–3704.

    Article  PubMed  Google Scholar 

  • Hermansson, M. and Marshall, K. C.: 1985, ‘Utilization of surface localized substrate by non-adhesive marine bacteria’, Microb. Ecol. 11, 91–105.

    Article  Google Scholar 

  • Hrudey, S. E., Huck, P. M., Payment, P., Gilham, R. W. and Hrudey, E. J.: 2002, ‘Walkerton: Lessons learned in comparison with waterborne outbreaks in the developed world’, J. Environ. Eng. Sci. 1, 397–407.

    Article  Google Scholar 

  • Huang, S. W., Chang, C. H., Tai, T. F. and Chang, T. C.: 1997, ‘Comparison of the β-Glucuronidase Assay and the Conventional Method for Identification of Escherichia coli on {eosin-methylene blue agar}’, Journal of Food Protection 60, 6–9.

    PubMed  Google Scholar 

  • Hübner, I., Steinmetz, I., Obst, U., Giebel, D. and Bitter-Suermann, D.: 1992, ‘Rapid determination of members of the family Enterobacteriaceae in drinking water by an immunological assay using a monoclonal antibody against enterobacterial common antigen’, Appl. Environ. Microbiol. 58, 3187–3191.

    PubMed  Google Scholar 

  • Iqbal, S., Robinson, J., Deere, D., Saunders, J. R., Edwards, C. and Porter, J.: 1997, ‘Efficiency of the polymerase chain reaction amplification of the uid gene for detection of Escherichia coli in contaminated water’, Lett. Appl. Microbiol. 24, 498–502.

    Article  PubMed  Google Scholar 

  • Jimenez, L., Muniz, I., Toranzos, G. A. and Hazen, T. C.: 1989, ‘Survival and activity of Salmonella typhimurium and Escherichia coli in tropical freshwater’, J. Appl. Bacteriol. 67, 61–69.

    PubMed  Google Scholar 

  • Juck, D., Ingram, J., Prévost, M., Coallier, J. and Greer, C.: 1996, ‘Nested PCR protocol for the rapid detection of Escherichia coli in potable water’, Can. J. Microbiol. 42, 862–866.

    PubMed  Google Scholar 

  • Kefford, B., Kjelleberg, S. and Marshall, K. C.: 1982, ‘Bacterial scavenging: Utilization of fatty acids localized at a solid-liquid interface’, Arch. Microbiol. 133, 257–260.

    Article  Google Scholar 

  • Kistemann, T., Claβ en, T., Koch, C., Dangendorf, F., Fischeder, R., Gebel, J., Vacacta, V. and Exner, M.: 2002, ‘Microbial {laod of drinking water reservoir tributaries during extreme rainfall and runoff}’, Appl. Environ. Microbiol. 68, 2188–2197.

    Article  PubMed  Google Scholar 

  • Kornacki, J. L. and Johnson, J. L.: 2001, ‘Enterobacteriaceae, Coliforms and Escherichia coli as {quality and safety indicators}’, in F. Downs (ed), Compendium of Methods for the Microbiological Examination of Foods, APHA. Washington DC.

  • Koster, W., Egli, T., Ashbolt, N., Botzenhart, K., Burlion, N., Endo, T., Grimont, P., Guillot, E., Mabilat, C., Newport, L., Niemi, M., Payment, P., Prescott, A., Renaud, P. and Rust, A.: 2003, ‘Analytical methods for microbiological water quality testing’, in Assessing Microbial Safety of Drinking Water: Improving Approaches and Methods, OECD/WHO Drinking Water Quality Series, IWA Publishing, London, pp. 237–295.

    Google Scholar 

  • Lebaron, P., Catala, P., Fajon, C., Joux, F., Baudart, J. and Bernard, L.: 1997, ‘A new sensitive, whole-cell hybridization technique for detection of bacteria involving a biotinylated oligunucleotide probe targeting rRNA and tyramide signal amplification’, Appl. Environ. Microbiol. 63, 3274–3278.

    Google Scholar 

  • LeChevallier, M. W., Cawthon, C. D. and Lee, R. G.: 1988, ‘Factors promoting survival of bacteria in chlorinated water supplies’, Appl. Environ. Microbiol. 54, 649–654.

    PubMed  Google Scholar 

  • LeChevallier, M. W.: 1990, ‘Coliform regrowth in drinking water: A review. Res. Technol’, J. Am. Water. Works Assoc. 82, 74–86.

    Google Scholar 

  • Leclerc, H. and Moriamez, J. C.: 1980, ‘Étude quantitative de la flore fécale de l’adulte et du nourrisson alimenté artificiellement’, Pathol. Biol. 28(4), 217–226.

    PubMed  Google Scholar 

  • Leclerc, H., Mossel, D. A., Edberg, S. C. and Struijk, C. B.: 2001, ‘Advances in the {bacteriology of the coliform group}: Their {suitability as markers of microbial water safety}’, Annual Reviews in Microbiology 55, 201–234.

    Article  Google Scholar 

  • Leclercq, A., Wanegue, C. and Baylac, P.: 2002, ‘Comparison of {fecal coliform agar and violet red bile lactose agar for fecal coliform enumeration in foods}’, Appl. Environ. Microbiol. 68, 1631– 1638.

    Article  PubMed  Google Scholar 

  • Lemarchand, K., Masson, L. and Brousseau, R.: 2004, ‘Molecular biology and DNA microarray technology for microbial quality monitoring of water’, Crit. Rev. Microbiol. 30, 145– 172.

    Article  PubMed  Google Scholar 

  • Leung, K. T., Mackereth, R., Tien, Y. C. and Topp, E.: 2004, ‘A comparison of AFLP and ERIC-PCR analyses for discriminating Escherichia coli from cattle, pig and human’, FEMS Microbiol. Ecol. 47, 111–119.

    Article  Google Scholar 

  • Levasseur, S., Husson, M. O., Leitz, R., Merlin, F., Laurent, F., Peladan, F., Drocourt, J. L., Leclerc, H. and Van Hoegaerden, M.: 1992, ‘Rapid detection of members of the family Enterobacteriaceae by a monoclonal antibody’, Appl. Environ. Microbiol. 58, 1524–1529.

    PubMed  Google Scholar 

  • Lopez-Torres, A. J., Hazen, T. C. and Toranzos, G. A.: 1987, ‘Distribution and in situ survival and activity of Klebsiella pneumoniae and Escherichia coli in tropical rain forest watershed’, Curr. Microbiol. 15, 213–218.

    Article  Google Scholar 

  • Lund, V.: 1996, ‘Evaluation of E.~coli as an indicator for the presence of Campylobacter jejuni and Yersinia enterocolitica in chlorinated and untreated oligotrophic lake water’, Water Res. 30, 1528–1534.

    Article  Google Scholar 

  • Manja, E. S., Maurya, M. S. and Rao, D. M.: 1982, ‘A simple field test for the detection of fecal pollution in drinking water’, Bull. WHO, 60, 797–801.

    PubMed  Google Scholar 

  • Marshall, K. C. and Blainey, B. L.: 1991, ‘Role of bacterial adhesion in biofilm formation and biocorrosion’, in H. C. Flemming (ed), Biofouling and Biocorrosion in Industrial Water Systems, Berlin: Springer-Verlag., pp. 29–46.

    Google Scholar 

  • Marshall, K. C.: 1988, ‘Adhesion and growth of bacteria at surfaces in oligotrophic habitats’, Can. J. Microbiol. 34, 503–506.

    Google Scholar 

  • Marshall, M. M., Naumovitz, D., Ortega, Y. and Sterling, C. R.: 1997, ‘Waterborne protozoan pathogens’, Clin. Microbiol. Rev. 10, 67–85.

    PubMed  Google Scholar 

  • Martins, M. T., Rivera, I. G., Clark, D. L., Stewart, M. H., Wolfe, R. L. and Olson, B. H.: 1993, ‘Distribution of uidA gene sequences in Escherichia coli isolates in water sources and comparison with the expression of beta-glucuronidase activity in 4-methylumbelliferyl-beta-D-glucuronide media’, Appl. Environ. Microbiol. 59(7), 2271–2276.

    PubMed  Google Scholar 

  • McLellan, S. L., Daniels, A. D. and Salmore, A. K.: 2001, ‘Clonal {populations of thermotolerant enterobacteriaceae in recreational water and their interference with fecal} Escherichia coli counts’, Appl. Environ. Microbiol. 67, 4934–4938.

    Article  PubMed  Google Scholar 

  • Min, J. and Baeumner: 2001, ‘Highly {sensitive and specific detection of viable} Escherichia coli in {drinking water}’, Analytical Biochemistry. 303, 186–193.

    Google Scholar 

  • Morris, R. D., Naumova, E. N. and Griffiths, J. K.: 1998, ‘Did Milwaukee experience waterborne cryptosporidiosis before the large outbreak in 1993?’, Epidemiology 9, 264–270.

    Article  PubMed  Google Scholar 

  • Noble, R. T. and Fuhrman, J. A.: 2001, ‘Enteroviruses detected by reverse transcriptase polymerase chain reaction from the coastal waters of Santa Monica Bay, California: Low correlation to bacterial indicator levels’, Hydrobiologia 460(1), 175–184.

    Article  Google Scholar 

  • Norton, C. D. and LeCevallier, M. W.: 2000, ‘A {pilot study of bacteriological population changes through potable water treatment and distribution}’, Appl. Environ. Microbiol. 66, 268– 276.

    PubMed  Google Scholar 

  • Obst, U., Hübner, I., Wecker, M. and Bitter-Suermann, D.: 1989, ‘Immunological method using monoclonal antibodies to detect Enterobacteriaceae in drinking water’, Aqua 38, 136–142.

    Google Scholar 

  • OECD (Organization for Economic Co-operation and Development): 2003, ‘Assessing Microbial Safety of Drinking Water: Improving Approaches and Methods’, OECD/WHO Drinking Water Quality Series, IWA Publishing, London.

  • Owens, J. H., Miltner, R. J., Rice, E. W., Johnson, C. H., Dahling, D. R., Schaefer, F. W. and Shukairy, H. M.: 2000, ‘Pilot-scale ozone inactivation of Cryptosporidium and other microorganisms in natural water’, Ozone Science {&} Engineering 22(5), 501–517.

    Google Scholar 

  • Payment, P. and Franco, E.: 1993, ‘Clostridium perfringens and somatic coliphages as indicators of the efficiency of drinking water treatment for viruses and protozoan cysts’, Appl. Environ. Microbiol. 59, 2418–2424.

    PubMed  Google Scholar 

  • Payment, P., Richardson, L., Siemiatycki, J., Dewar, R., Edwardes, M. and Franco, E.: 1991a, ‘A randomized trial to evaluate the risk of gastrointestinal disease due to consumption of drinking water meeting current microbiological standards’, Am. J. Public Health 81, 703–708.

    Google Scholar 

  • Payment, P.: 1999, ‘Poor efficacy of residual chlorine disinfectant in drinking water to inactivate waterborne pathogens in distribution systems’, Canadian Journal of Microbiology 45(9), 709–715.

    Article  PubMed  Google Scholar 

  • Payment, P., Plante, R. and Cejka, P.: 2001, ‘Removal of indicator bacteria, human enteric viruses, Giardia cysts and Cryptosporidium oocysts at a large wastewater primary treatment facility’, Can. J. Microbiol. 47(3), 188–193.

    Article  PubMed  Google Scholar 

  • Petit, M., George, I. and Servais, P.: 2000, ‘Survival of Escherichia coli in freshwater: β -D-Glucoronidase activity measurements and characterization of cellular states’, Canadian Journal of Microbiology 46, 679–684.

    Article  PubMed  Google Scholar 

  • Peyton, B. M. and Characklis, W. G.: 1992, ‘Kinetics of biofilm detachment’, Water Sci. Technol. 26, 1995–1998.

    Google Scholar 

  • Power, K. and Marshall, K. C.: 1988, ‘Cellular growth and reproduction of marine bacteria on surface-bound substrate’, Biofouling 1, 163–74.

    Google Scholar 

  • Prescott, A. M. and Fricker, C. R.: 1999, ‘Use of PNA oligonucleotides for the in situ detection of Escherichia coli in water’, Mol. Cell. Probes 13, 261–268.

    Article  PubMed  Google Scholar 

  • Productivity Commission: 2000, Arrangements for Setting Drinking Water Standards, International Benchmarking, AusInfo, Canberra.

    Google Scholar 

  • Quignon, F., Kiene, L., Levi, Y., Sardin, M. and Schwartzbrod, L.: 1997, ‘Virus behaviour within a distribution system’, Water Sci. Technol. 35, 311–18.

    Article  Google Scholar 

  • Rajala, R. L. and Heinonen-Tanski, H.: 1998, ‘Survival and {transfer of faecal indicator organisms of wastewater effluents in receiving lake waters}’, Wat. Sci. Tech. 38(12), 191–194.

    Article  Google Scholar 

  • Regnault, B., Martin-Delautre, S., Lejay-Collin, M., Lefèvre, M. and Grimont, P. A. D.: 2000, ‘Oligonucleotide probe for the visualization of Escherichia coli/Escherichia fergusonii cells by in situ hybridization: Specificity and potential application’, Res. Microbiol. 151, 521– 533.

    Article  PubMed  Google Scholar 

  • Ridgeway, H. F. and Olson, B. H.: 1981, ‘Scanning electron microscope evidence for bacterial colonization of a drinking-water distribution system’, Appl. Environ. Microbiol. 41, 274–287.

    PubMed  Google Scholar 

  • Rippey, S. R. and Cabelli, V. J.: 1989, ‘Use of thermotolerant Aeromonas group for the trophic classification of freshwater’, Water Res. 23, 1107–1114.

    Article  Google Scholar 

  • Rompré, A., Servais, P., Baudart, J., de-Roubin, M. and Laurent, P.: 2002, ‘Detection and enumeration of coliforms in drinking water: Current methods and emerging approaches’, Journal of Microbiological Methods 49(1), 31–54.

    Article  PubMed  Google Scholar 

  • Salyers, A. and Whitt, D.: 2002, Bacterial Pathogenesis: A Molecular Approach. 2nd Edn’, ASM Press Washington, DC.

    Google Scholar 

  • Seyfried, P. and Harris, E.: 1990, ‘Bacteriological Characterization of Feces and Source Differentiation’, Water Resources Branch, Ontario Ministry of the Environment. Queen’s Printer for Ontario.

  • Skraber, S., Gantzer, C., Maul, A. and Schwartzbrod, L.: 2002, ‘Fates of bacteriophages and bacterial indicators in the Moselle river (France)’, Water Res. 36(14), 3629–3637.

    Article  PubMed  Google Scholar 

  • Sorenson, D. L., Eberl, S. G. and Diksa, R. A.: 1989, ‘Clostridium perfringens as a point source indicator in non-point-polluted streams’, Wat. Res. 23, 191–197.

    Article  Google Scholar 

  • Stevens, M., Ashbolt, N. and Cunliffe, D.: 2001, Microbial Indicators of Water Quality – An NHMRC Discussion Paper.

  • Sueiro, R. A., Araujo, M., Santos, C. J., Gomez, M. J. and Garrido, M. J.: 2001, ‘Evaluation of Coli-ID and MUG Plus media for recovering Escherichia coli and other coliform bacteria from groundwater samples’, Water Sci Technol. 43(12), 213–216.

    Google Scholar 

  • Szewzyk, U., Manz, W., Amann, R., Schleifer, K. H. and Stenström, T. A.: 1994, ‘Growth and in situ detection of a pathogenic Escherichia coli in biofilms of a heterotrophic water-bacterium by use of 16S- and 23S-rRNA-directed fluorescent oligonucleotide probes’, FEMS Microbiol. Ecol. 13, 169–176.

    Article  Google Scholar 

  • Szewzyk, U. and Schink, B.: 1987, ‘Surface colonization by and life cycle of Pelobacter acidigallici studied in a continuous-flow microchamber’, J. Gen. Microbiol. 134, 183–190.

    Google Scholar 

  • Szwezyk, U., Szewzyk, R., Manz, W. and Schleifer, K. H.: 2000, ‘Microbiological {safety of drinking water}’, Annu. Rev. Microbiol. 54, 81–127.

    Article  PubMed  Google Scholar 

  • Tani, K., Kurokawa, K. and Nasu, M.: 1998, ‘Development of a direct in situ PCR method for detection of specific bacteria in natural environments’, Appl. Environ. Microbiol. 64, 1536–1540.

    PubMed  Google Scholar 

  • Tenover, F. C., Arbeit, R. D. and Goering, R. V.: 1997, ‘How to select and interpret molecular strain typing methods for epidemiological studies of bacterial infections: A review for healthcare epidemiologists’, Infection control and hospital epidemiology 18, 426–439.

    PubMed  Google Scholar 

  • Toranzos, G. A., McFeters, G. A. and Borrego, J. J.: 2002, ‘Detection of microorganisms in environmental freshwaters and drinking waters’, in C. J. Hurst, R. L. Crawford, G. R. Knudsen, M. J. McInerney and L. D. Stetzenbach (eds), Manual of Environmental Microbiology, 2nd ed, ASM Press, Washington, D. C., pp. 205–219.

    Google Scholar 

  • Tortorello, M. L. and Reineke, K. F.: 2000, ‘Direct Enumeration of Escherichia coli and enteric bacteria in water, beverages and sprouts by 16S rRNA in situ hybridization’, Food Microbiology 17, 305–313.

    Article  Google Scholar 

  • Tryland, I. and Fiksdal, L.: 1998, ‘Enzyme Characteristics of the β -D-Galactosidase- and β -D-Glucoronidase-Positive Bacteria and Their Interference in Rapid Methods for Detection of Waterborne Coliforms and Escherichia coli’, Applied and Environmental Microbiology 64, 1018–1023.

    PubMed  Google Scholar 

  • Van Poucke, S. O. and Nelis, H. J.: 2000a, ‘Rapid detection of fluorescent and chemiluminescent total coliforms and Escherichia coli on membrane filters’, Journal of Microbiological Methods 42(3), 233–244.

    Article  Google Scholar 

  • Van Poucke, S. O. and Nelis, H. J.: 2000b, ‘A 210-min solid phase cytometry test for the enumeration of Escherichia coli in drinking water’, J. Appl. Microbiol. 89, 390–396.

    Article  Google Scholar 

  • Van Pouke, S. O. and Nelis, H. J.: 1997, ‘Limitations of Highly Sensitive Enzymatic Presence-Absence Tests for Detection of Waterborne Coliforms and Escherichia coli’, Applied and Environmental Microbiology 63, 771–774.

    Google Scholar 

  • Wadowsky, R. M. and Yee, R. B.: 1983, ‘Satellite growth of Legionella pneumophila with an environmental isolate of Flavobacterium breve’, Appl. Environ. Microbiol. 46, 1447–1449.

    PubMed  Google Scholar 

  • Yu, H.: 1998, ‘Comparative studies of magnetic particle-based solid phase fluorogenic and electrochemiluminescent immunoassay’, Journal of Immunological Methods 218, 1–8.

    Article  PubMed  Google Scholar 

  • Zaccone, R., Crisafi, E. and Caruso, G.: 1995, ‘Evaluation of fecal pollution in coastal Italian waters by immunofluorescence’, Aquat. Microb. Ecol. 9, 79–85.

    Google Scholar 

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Correspondence to Kam Tin Leung.

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Tallon, P., Magajna, B., Lofranco, C. et al. Microbial Indicators of Faecal Contamination in Water: A Current Perspective. Water Air Soil Pollut 166, 139–166 (2005). https://doi.org/10.1007/s11270-005-7905-4

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