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Natural Biofilms in Freshwater Ecosystem: Indicators of the Presence of Polycyclic Aromatic Hydrocarbons

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Abstract

Hydrophobic organic compounds are common in the environment, especially in water bodies like rivers and lakes. Generally, due to their physico-chemical characteristics, mainly to hydrophobicity, these compounds are adsorbed by suspended material or other compartments which provide compatibility. Thus, compounds such as polycyclic aromatic hydrocarbons (PAHs) are rapidly adsorbed onto suspended material or even naturally occurring biofilms in water bodies. Biofilms can be defined as complex structures with cells and aggregates of cells. The extracellular polymers present empty spaces that can be filled by water. The biofilm is a sessile microbial community with several kinds of organisms such as bacteria, protozoa, fungi, algae, and extracellular polymeric substances, which may be found on almost any surface exposed to water. Here, biofilms were used to monitor the presence of PAHs in the Barigui River in Curitiba, Brazil. For the measurements and collection of representative microcoenoses, a biofilm sampling device was designed consisting of six glass plates installed in an open polyvinyl chloride pipe of 30 cm diameter and 60 cm length. The sampling device was exposed in the Barigui River for 2 weeks campaigns. The formed biofilm was treated and chemical analysis was performed for PAHs determination. The results showed that biofilms can trap most of the PAHs, especially those with high K ow values (octanol–water partition coefficient). Four campaigns were conducted. The total PAHs concentration ranged from 11,204.34 ± 560.12 to 45,846.90 ± 2,290.45 ng/g. According to the isomers ratio, the main source of PAHs in the first and second campaign was of pyrolytic origin, in other words, the PAHs were by-products from burning of light-refined oil products (gasoline and diesel oil). Meanwhile, the other campaigns revealed that the main source is of petrogenic origin. However, the possibility of both sources is not discarded considering the scenario studied and the records of sediments samples. Most of the investigations carried out focused on the loading of river sediments and suspended solids, but the biofilms might detect the amount that could be taken up by benthic organisms, for instance.

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References

  • Agbozu, I. E., & Opuene, K. (2009). Occurrence and diagenetic evolution of perylene in the sediments of Oginigba Creek, Southern Nigeria. International Journal Environmental Research, 3(1), 117–120.

    CAS  Google Scholar 

  • Boonyatumanond, R., Wattayakorn, G., Togo, A., & Takada, H. (2006). Distribution and origins of polycyclic aromatic hydrocarbons (PAHs) in riverine, estuarine, and marine sediments in Thailand. Marine Pollution Bulletin, 52(8), 942–956.

    Article  CAS  Google Scholar 

  • Budzinski, H., Bellocq, I. J. J., Pierard, C., & Garrigues, P. (1997). Evaluation of sediment contamination by polycyclic aromatic hydrocarbons in the Gironde estuary. Marine Chemistry, 58(1–2), 85–97.

    Article  CAS  Google Scholar 

  • Baumard, P., Budzinski, H., Michona, Q., Garrigues, P., Burgeot, T., & Bellocq, J. (1998). Origin and bioavailability of PAHs in the Mediterranean Sea from mussel and sediment records. Estuarine, Coastal and Shelf Science, 47(1), 77–90.

    Article  CAS  Google Scholar 

  • Costerton, J. W., Lewandowski, Z., Caldwell, D. E., Korber, D. R., & Lappin-Scott, H. M. (1995). Microbial biofilms. Annual Reviews Microbiology, 49, 711–745.

    Article  CAS  Google Scholar 

  • Donati, J., & Person, A. (1989). Exposition B la pollution automobile en milieu urbain. Pollution Atmospheric, 121, 9–17.

    CAS  Google Scholar 

  • Doong, R., & Lin, Y. (2004). Characterization and distribution of polycyclic aromatic hydrocarbon contaminations in surface sediment and water from Gao-Ping River, Taiwan. Water Research, 38(7), 1733–1744.

    Article  CAS  Google Scholar 

  • Fang, G. C., Chang, K. F., Lu, L., & Bai, H. (2004). Estimation of PAHs dry deposition and BaP toxic equivalency factors (TEFs) study at urban, industrial park and rural sampling in central Taiwan, Taichuang. Chemosphere, 55(6), 787–796.

    Article  CAS  Google Scholar 

  • Fang, M. D., Hsieh, P. C., Ko, F. C., Baker, J. E., & Lee, C. L. (2007). Sources and distribution of polycyclic aromatic hydrocarbons in the sediments of Kaoping River and submarine canyon system, Taiwan. Marine Pollution Bulletin, 54(8), 1179–1189.

    Article  CAS  Google Scholar 

  • Fasnacht, M. P., & Blough, N. V. (2002). Aqueous photodegradation of polycyclic aromatic hydrocarbons. Environmental Science and Technology, 36(20), 4364–4369.

    Article  CAS  Google Scholar 

  • Flemming, H. C. (2003). Role and levels of real-time monitoring for successful anti-fouling strategies—an overview. Water Science and Technology, 47(5), 1–9.

    CAS  Google Scholar 

  • Friese, K., Mages, M., Neu, T. R. (1997). Determination of heavy metals in biofilms from the River Elbe by total reflection X-ray fluorescence spectrometry. Spectrochimica Acta Part B, 52(7), 1019–1025.

    Google Scholar 

  • Froehner, S., Maceno, M., Cardoso da Luz, E., Souza, D. B., & Scurupa, K. (2009). Distribution of polycyclic aromatic hydrocarbons in marine sediments and their potential toxic effects. Environmental Monitoring and Assessment. doi:10.1007/s10661-009-1104-5.

  • Froehner, S., & Martins, R. F. (2007). Assessment of fate and bioaccumulation of benzo(a)pyrene by computer modeling. Quimica Nova, 31(5), 1089–1093.

    Article  Google Scholar 

  • Froehner, S., Souza, D. B., Scurupa, K., & Rosa, E. C. (2010). Tracking anthropogenic inputs in Barigui River, Brazil using biomarkers. Water, Air, and Soil Pollution, 210(1–4), 33–41.

    Article  CAS  Google Scholar 

  • Froehner, S., & Martins, R. F. (2008). Evaluation of the chemical composition of sediments from the Barigui River in Curitiba, Brazil. Quimica Nova, 31(8), 2020–2026.

    Article  Google Scholar 

  • Fuchs, S., Haritopoulou, T., & Wilhelmi, M. (1996). Biofilms in freshwater ecosystems and their use as a pollutant monitor. Water Science and Technology, 34(7–8), 137–140.

    Article  CAS  Google Scholar 

  • Fuchs, S., Haritopoulou, T., Shafer, M., & Wilhelmi, M. (1997). Heavy metals in freshwater ecosystems introduced by urban rainwater runoff—monitoring of suspended solids, river sediments and biofilms. Water Science and Technology, 36(8–9), 277–282.

    Article  CAS  Google Scholar 

  • Gigliotti, C., Brunciak, P. A., Dachs, J., Glenniv, T. R., Nelson, E. D., Totten, L. A., & Eisenreich, S. J. (2002). Air–water exchange of polycyclic aromatic hydrocarbons in the New York, New Jersey, USA, Harbor Estuary. Environmental Toxicology and Chemistry, 21(1), 235–244.

    CAS  Google Scholar 

  • IARC. (1991). Monographs on the evaluation of carcinogenic risk of chemicals to humans (Vol. 53, p. 440). Lyon: International Agency for Research on Cancer.

    Google Scholar 

  • Li, G., Xia, X., Yang, Z., Wang, R., & Voulvoulis, N. (2006). Distribution and sources of polycyclic aromatic hydrocarbons in the middle and lower reaches of the Yellow River, China. Environmental Pollution, 144(3), 985–993.

    Article  CAS  Google Scholar 

  • Lee, H. B., & Peart, T. E. (1991). Determination of resin and fatty acids in sediments near pulp mill locations. Journal of Chromatography. A, 547, 315–323.

    Article  CAS  Google Scholar 

  • Mackay, D. (1992). Correlation of bioconcentration factors. Environmental Science and Technology, 16(8), 274–278.

    Google Scholar 

  • Peyton, B. M., Viamajala, S., Richards, L. A., & Petersen, J. N. (2007). Solubilization, solution equilibria, and biodegradation of PAH’s under thermophilic conditions. Chemosphere, 66(6), 1094–1106.

    Article  Google Scholar 

  • Shi, Z., Tao, S., Pan, B., Liu, W. X., & Shen, W. R. (2007). Partitioning and source diagnostics of polycyclic aromatic hydrocarbons in rivers in Tianjin, China. Environmental Pollution, 146(3), 492–500.

    Article  CAS  Google Scholar 

  • Schwarzenbach, R., Gschwend, P., & Imboden, D. (2003). Environmental organic chemistry. Hoboken: Wiley.

    Google Scholar 

  • Simpsom, M. J., Chefetz, B., Deshmukh, A. P., & Hatcher, P. G. (2005). Comparison of polycyclic aromatic hydrocarbon distributions and sedimentary organic matter characteristics in contaminated, coastal sediments from Pensacola Bay, Florida. Marine Environmental Research, 59(1), 139–163.

    Article  Google Scholar 

  • Soclo, H. H., Garrigues, P. H., & Ewald, M. (2000). Origin of polycyclic aromatic hydrocarbons (PAHs) in coastal marine sediments: case studies in Cotonou (Benin) and Aquitaine (France) areas. Marine Pollution Bulletin, 40(5), 387–396.

    Article  CAS  Google Scholar 

  • Schorer, M., & Eisele, M. (1997). Accumulation of inorganic and organic pollutants by biofilms in the aquatic environment. Water, Air, and Soil Pollution, 99(1–4), 651–659.

    CAS  Google Scholar 

  • Ternes, T. A., Stumpf, M., Mueller, J., Haberer, K., Wilken, R. D., & Servos, M. (1999). Behavior and occurrence of estrogens in municipal sewage treatment plants. I. Investigations in Germany, Canada and Brazil. Science of the Total Environmental, 225(1–2), 81–90.

    CAS  Google Scholar 

  • Yunker, M. B., Macdonald, R. W., Goyette, D., Paton, D. W., Fowler, B. R., Sullivan, D., & Boyd, J. (1999). Natural and anthropogenic inputs of hydrocarbons to the Strait of Georgia. Science of the Total Environment, 225(3), 181–209.

    Article  CAS  Google Scholar 

  • Yunker, M. B., Macdonald, R. W., Vingarzan, R., Mitchell, R. H., Goyette, D., & Sylvestre, S. (2002). PAHs in the Fraser river basin: a critical appraisal of PAH ratios as indicators of PAH source and composition. Organic Geochemistry, 33(4), 489–515.

    Article  CAS  Google Scholar 

  • Van der Heijden, S. A., & Jonker, M. T. O. (2009). PAH bioavailability in field sediments: comparing different methods for predicting in situ bioaccumulation. Environmental Science and Technology, 43(11), 3757–3763.

    Article  Google Scholar 

  • Wolska, L., Zygmunt, B., & Namiesnik, J. (2003). Organic pollutants in the Odra river ecosystem. Chemosphere, 53(5), 561–569.

    Article  CAS  Google Scholar 

  • Zakaria, M. P., Takada, H., Tsutsumi, S., Ohno, K., Yamada, J., Kouno, E., & Kumata, H. (2002). Distribution of polycyclic aromatic hydrocarbons (PAHs) in rivers and estuaries in Malaysia: a widespread input of petrogenic PAHs. Environmental Science and Technology, 36(5), 1907–1918.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors thank CNPq for financial aid (Processos 577060/2008-2 and 471183/2010-0). We also thank the helpful discussion of Dr. Cristovão Scapulatempo Fernandes.

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Authors and Affiliations

  1. Department of Environmental Engineering, Federal University of Parana, Curitiba, Paraná, Brazil, 81531-980

    Sandro Froehner

  2. Department of Hydraulics and Sanitation, Federal University of Parana, Curitiba, Paraná, Brazil, 81531-980

    Karina Scurupa Machado, Luiz Fernando Dombroski, Anderson Correa Nunes, Regina Tiemy Kishi, Tobias Bleninger & Juan Sanez

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  1. Sandro Froehner
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  2. Karina Scurupa Machado
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  3. Luiz Fernando Dombroski
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  6. Tobias Bleninger
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Correspondence to Sandro Froehner.

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Froehner, S., Machado, K.S., Dombroski, L.F. et al. Natural Biofilms in Freshwater Ecosystem: Indicators of the Presence of Polycyclic Aromatic Hydrocarbons. Water Air Soil Pollut 223, 3965–3973 (2012). https://doi.org/10.1007/s11270-012-1164-y

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