Summary
Three strains of Dunaliella salina (I, G and A) were cultivated under the climatic conditions of Iran, in open ponds to compare the β-carotene production and the specific rate of growth. The experiments were accomplished in two separate stages. In the first stage, the cells were grown in ponds on nutrient-rich medium containing 2 M NaCl to obtain the necessary biomass. In the second stage, cells were stressed on nutrient-poor medium containing 2.5 M NaCl for β-carotene induction. The results showed that the specific growth rate of strain I was the highest during the first stage, whereas during the second stage, the growth rates of three strains were approximately the same. The overall results indicated that strain G had the highest potential for β-carotene accumulation of the strains tested and hence it was concluded that this strain is more suitable for outdoor cultivation under the climatic conditions of Iran than the other two.
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Acknowledgements
This work was supported by the Faculty of Postgraduate Studies of University of Isfahan, Isfahan, Iran. We thank to Dr Gert Schansker and Professor Reto Strasser (University of Geneva) for their linguistic help.
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Tafreshi, A.H., Shariati, M. Pilot culture of three strains of Dunaliella salina for β-carotene production in open ponds in the central region of Iran. World J Microbiol Biotechnol 22, 1003–1006 (2006). https://doi.org/10.1007/s11274-006-9145-1
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DOI: https://doi.org/10.1007/s11274-006-9145-1