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Tannase production by Aspergillus fumigatus MA under solid-state fermentation

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Abstract

A tannase yielding fungal culture identified as Aspergillus fumigatus MA was isolated from the effluent collected from a local small scale tannery. The fungal culture produced high yields of extracellular tannase under solid-state fermentation (SSF) using different agro forest residues such as Amla leaves (Phyllanthus emblica), Ber leaves (Zyzyphus mauritiana), Jamun leaves (Syzygium cumini), Jamoa leaves (Syzygium sp.) and Keekar leaves (Acacia nilotica). Among different substrates used, Jamun leaves yielded maximal extra-cellular production of tannase. Various parameters were studied to optimize the extracellular yield of tannase under SSF. The maximum yield of 174.32 U g−1 was obtained at 25°C after 96 h of incubation at pH 5.0. The tap water was used as a moistening agent. A substrate to tap water ratio of 1:1 was found to best for tannase production. Supplementation of the medium with ammonium sulfate as nitrogen source had enhanced tannase production whereas glucose had decreased the enzyme production. This is the first report on production of tannase by Aspergillus fumigatus MA, giving a much higher yield of enzyme under SSF with Jamun leaves as the substrate.

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Acknowledgments

We are thankful to Prof. Ashok Aggarwal from Department of Botany, Kurukshetra University, Kurukshetra for the identification of the culture. The junior research fellowship under Rajiv Gandhi National Fellowship scheme (University Grant Commission) sponsored by the Ministry of Social Justice & Empowerment and Ministry of Tribal Affairs, Govt. of India to the first author is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Biotechnology, Kurukshetra University, Kurukshetra, 136 119, Haryana, India

    Manjit, Anita Yadav & Anil Kumar

  2. Department of Microbiology, Kurukshetra University, Kurukshetra, 136 119, Haryana, India

    N. K. Aggarwal

  3. Dairy Microbiology Division, National Dairy Research Institute, Karnal, 132001, Haryana, India

    Krishan Kumar

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  1. Manjit
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  2. Anita Yadav
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Correspondence to Anita Yadav.

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Manjit, Yadav, A., Aggarwal, N.K. et al. Tannase production by Aspergillus fumigatus MA under solid-state fermentation. World J Microbiol Biotechnol 24, 3023–3030 (2008). https://doi.org/10.1007/s11274-008-9847-7

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