Abstract
Bacterial community succession in the start-up of a large-scale, completely-mixed composting reactor was analyzed by 16S rRNA gene (16S rDNA) clone analysis and denaturing gradient gel electrophoresis (DGGE) combined with measurements of temperature, pH, moisture contents, and decomposing rate. DGGE analysis and physicochemical parameters showed that bacterial community succession occurred in four phases; (1) at the start of operation and pH decreasing period (day 0–3), (2) pH decreased and increased period (day 4–11), (3) middle term, moisture content decreasing and maximum temperature increased period (day 12–16) and (4) latter term, temperature decreasing period (day 17–24). Lactobacillus spp. and Bacillus coagulans were detected from the initial phase and middle term, respectively. 16S rDNA clone analysis showed that the dominant bacteria shifted from the order “Lactobacillales” to Bacillales and Actinomycetales. The order “Lactobacillales” was unique which may be caused by using the plastic bottle flakes (polyethylene terephthalate, PET) as bulking agent.
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Abbreviations
- BLAST:
-
Basic local alignment search tool
- DGGE:
-
Denaturing gradient gel electrophoresis
- rDNA:
-
rRNA gene
- r.p.m.:
-
Revolutions per minute
- VS:
-
Volatile substrate
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Acknowledgments
We thank Mr. Shinichiro Osa for technical assistance, Dr. Teruaki Yoshida for correcting English. This work was supported by the “University-Industry Joint Research” Project for Private Universities and a matching fund subsidy from MEXT (Ministry of Education, Culture, Sports, Science and Technology) of Japan, 2004–2008.
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Watanabe, K., Nagao, N., Toda, T. et al. The dominant bacteria shifted from the order “Lactobacillales” to Bacillales and Actinomycetales during a start-up period of large-scale, completely-mixed composting reactor using plastic bottle flakes as bulking agent. World J Microbiol Biotechnol 25, 803–811 (2009). https://doi.org/10.1007/s11274-008-9952-7
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DOI: https://doi.org/10.1007/s11274-008-9952-7