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Optimization of cultural conditions for tannase production by Pseudomonas aeruginosa IIIB 8914 under submerged fermentation

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Abstract

A tannase yielding bacterial strain was isolated from soil sample collected from the area situated nearby small-scale tannery. It was identified as Pseudomonas aeruginosa IIIB 8914. The bacterial strain produced extra-cellular tannase under sub-merged fermentation (Smf) using amla (Phyllanthus emblica), keekar (Acacia nilotica), jamoa (Eugenia cuspidate) and jamun (Syzygium cumini) leaves. Among different substrates, amla and keekar leaves resulted in maximal extra-cellular production of tannase. Various process parameters were studied to optimize the extra-cellular yield of tannase under Smf. Maximum yield of tannase i.e., 13.65 and 12.90 U/ml was obtained when Smf was carried out using amla and keekar leaves (2% w/v) respectively in minimal media supplemented with MgSO4·7H2O (amla)/HgCl2 (keekar), NH4NO3 and 0.2% Tween 80; inoculated with 2% cell suspension, and incubated at 37°C for 24 h. The bacterial strain produced about 2 times (13.65 U/ml) higher yield of tannase than the highest reported yield of tannase (6 U/ml). Our finding suggests that agro residues in the form of amla and keekar leaves can be one of the best and cost effective alternatives to the costly pure tannic acid for industrial production of microbial tannase.

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Acknowledgments

The senior research fellowship under Rajiv Gandhi National Fellowship scheme (University Grant Commission) sponsored by the Ministry of Social Justice & Empowerment and Ministry of Tribal Affairs, Govt. of India to the first author is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Biotechnology, Kurukshetra University, Kurukshetra, Haryana, 136119, India

    Manjit K. Selwal, Anita Yadav & S. K. Gautam

  2. Dairy Microbiology Division, National Dairy Research Institute, Karnal, Haryana, 132001, India

    Krishan K. Selwal

  3. Department of Microbiology, Kurukshetra University, Kurukshetra, Haryana, 136119, India

    N. K. Aggarwal

  4. Department of Biochemistry, Kurukshetra University, Kurukshetra, Haryana, 136119, India

    Ranjan Gupta

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  1. Manjit K. Selwal
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  2. Anita Yadav
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  3. Krishan K. Selwal
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  4. N. K. Aggarwal
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  5. Ranjan Gupta
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  6. S. K. Gautam
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Correspondence to Anita Yadav.

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Selwal, M.K., Yadav, A., Selwal, K.K. et al. Optimization of cultural conditions for tannase production by Pseudomonas aeruginosa IIIB 8914 under submerged fermentation. World J Microbiol Biotechnol 26, 599–605 (2010). https://doi.org/10.1007/s11274-009-0209-x

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  • DOI: https://doi.org/10.1007/s11274-009-0209-x

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