Abstract
Oil-polluted soils were sampled from National Iranian South Oil Company (NISOC) for isolation and screening of C–S and not C–C targeted Dibenzothiophene (DBT) degrading microorganisms. Microbacterium sp. NISOC-06, a C–S targeted DBT degrading bacterium, was selected and its desulfurization ability was studied in aqueous phase and water-gasoline biphasic systems. The 16srRNA gene was amplified using universal eubacteria-specific primers, PCR product was sequenced and the sequence of nearly 1,500 bp 16srDNA was studied. Based on Gas Chromatography results Microbacterium sp. NISOC-06 utilized 94.8% of 1 mM DBT during the 2 weeks of incubation. UV Spectrophotometry and biomass production measurements showed that the Microbacterium sp. NISOC-06 was not able to utilize DBT as a carbon source. There was no accumulation of phenolic compounds as Gibb’s assay showed. Biomass production in a biphasic system for which DBT-enriched gasoline was used as the sulfur source indicated the capability of Microbacterium sp. NISOC-06 to desulfurize gasoline.
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Acknowledgment
This work was kindly supported by the vice chancellor for research office of Shahid Chamran Univesity, Iran and National Iranian South Oil Company (NISOC), Department of Research and Development and Department of Chemicals and Laboratories. Great thanks to Seied Abbas Dibaj, GC-man of gas chromatography laboratory of NISOC.
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Papizadeh, M., Ardakani, M.R., Ebrahimipour, G. et al. Utilization of dibenzothiophene as sulfur source by Microbacterium sp. NISOC-06 . World J Microbiol Biotechnol 26, 1195–1200 (2010). https://doi.org/10.1007/s11274-009-0288-8
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DOI: https://doi.org/10.1007/s11274-009-0288-8