Abstract
A novel thermostable, halostable carboxymethylcellulase (CMCase) from a marine bacterium Bacillus licheniformisAU01 was purified 10.4-fold with 18% yield with a specific activity of 88.43 U/mg and the molecular weight was estimated as 37 kDa. The enzyme was optimally active at pH 9–10 and temperature 50–60°C and it was most stable up to pH 12 and 20–30% of NaCl concentration. The enzyme activity was reduced when treated with Hg2+, Fe2+ and EDTA and stimulated by Co2+, Mn2+, Mg2+ and Ca2+. Various cationic, anionic detergents and commercial detergents were not much affected CMCase activity.
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Annamalai, N., Thavasi, R., Vijayalakshmi, S. et al. A novel thermostable and halostable carboxymethylcellulase from marine bacterium Bacillus licheniformisAU01. World J Microbiol Biotechnol 27, 2111–2115 (2011). https://doi.org/10.1007/s11274-011-0674-x
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DOI: https://doi.org/10.1007/s11274-011-0674-x