Skip to main content

Advertisement

Log in

Influence of co-inoculation of bacteria-cyanobacteria on crop yield and C–N sequestration in soil under rice crop

  • Original Paper
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

The performance of three selected bacterial strains—PR3, PR7 and PR10 (Providencia sp., Brevundimonas sp., Ochrobacterium sp.) and three cyanobacterial strains CR1, CR2 and CR3 (Anabaena sp., Calothrix sp., Anabaena sp.), and their combinations was evaluated in a pot experiment with rice variety Pusa-1460, comprising 51 treatments along with recommended fertilizer controls. Highest yield enhancement of 19.02% was recorded in T12 (CR2), over control, while significant enhancement in nitrogen fixing potential was recorded in treatments involving combination of bacterial-cyanobacterial strains—T37 (PR3 + CR1 + CR3) and T21 (PR7 + CR1). Organic carbon was significantly increased in all microbe-inoculated treatments, which could be correlated with microbial biomass carbon values and activities of all the enzymes tested in our study. Also, panicle weight and plant biomass were highly correlated with soil microbial carbon. Comparative evaluation revealed the superior performance of strains CR2, CR1 (both Anabaena sp.) and PR10 (Ochrobacterium sp.) in increasing the growth and grain yield of rice and improving soil health, besides N (nitrogen) savings of 40–80 kg ha−1. The study for the first time illustrated the positive effects of co-inoculation of bacterial and cyanobacterial strains for integrated nutrient management of rice crop.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Adesemoye A, Torbert H, Kloepper J (2009) Plant growth-promoting rhizobacteria allow reduced application rates of chemical fertilizers. Microb Ecol 58:921–929

    Article  CAS  Google Scholar 

  • Allison SD, Jastrow JD (2006) Activities of extracellular enzymes in physically isolated fractions of restored grassland soils. Soil Biol Biochem 38:3245–3256

    Article  CAS  Google Scholar 

  • Artursson V, Finlay RD, Jansson JK (2006) Interactions between arbuscular mycorrhizal fungi and bacteria and their potential for stimulating plant growth. Environ Microbiol 8:1–10

    Article  CAS  Google Scholar 

  • Asghar HN, Zahir ZA, Arshad M, Khalig A (2002) Plant growth regulating substances in the rhizosphere: microbial production and functions. Advan Agron 62:146–151

    Google Scholar 

  • Ashrafuzzaman M, Hossen FA, Razi IM, Hoque MA, Islam MZ, Shahidullah SM, Meon S (2009) Efficiency of plant growth-promoting rhizobacteria (PGPR) for the enhancement of rice growth. Afr J Biotech 8(7):1247–1252

    CAS  Google Scholar 

  • Averill C, Finzi A (2011) Increasing plant use of organic nitrogen with elevation is reflected in nitrogen uptake rates and ecosystem delta15N. Ecology 92(4):883–891

    Google Scholar 

  • Azcon R, Barea JM (2010) Mycorrhizosphere interactions for legume improvement. In: Khanf MS, Zaidi A, Musarrat J (eds) Microbes for legume improvement. Springer, Vienna, pp 237–271

    Chapter  Google Scholar 

  • Bernier J, Atlin GN, Serraj R, Kumar A, Spaner D (2008) Breeding upland rice for drought resistance. J Sci Food Agric 88:927–939

    Article  CAS  Google Scholar 

  • Biswas JC, Ladha JK, Dazzo FB, Yanni YG, Rolfe BG (2000) Rhizobial inoculation influences seedling vigor and yield bv. trifolii and rice roots and assessment of its potential to promote rice growth. Plant Soil 194:99–114

    Google Scholar 

  • Boddey RM, Dobereiner J (1995) Nitrogen fixation associated with grasses and cereals, recent progress and perspectives for the future. Fert Res 42:241–250

    Article  CAS  Google Scholar 

  • Casida LE Jr, Klein DA, Santoro T (1964) Soil dehydrogenase activity. Soil Sci 98:371–376

    Article  CAS  Google Scholar 

  • Correa JD, Barrios ML, Galdona RP (2004) Screening for plant growth-promoting rhizobacteria in Chamaecytisus proliferus (tagasaste), a forage tree-shrub legume endemic to the Canary Islands. Plant Soil 266:75–84

    Google Scholar 

  • De Datta SK, Buresh RJ (1989) Integrated nitrogen management in irrigated rice. Adv Soil Sci 10:143–169

    Article  Google Scholar 

  • Dey R, Pal KK, Bhatt DM, Chauhan SM (2004) Growth promotion and yield enhancement of peanut (Arachis hypogaea L.) by application of plant growth-promoting rhizobacteria. Microbiol Res 159:371–394

    Article  CAS  Google Scholar 

  • Elliot LF, Lynch JM (1984) Pseudomonas as a factor in the growth of winter wheat (Triticum aestivum L.). Soil Biochem 16:69–71

    Article  Google Scholar 

  • Frank A, Ryan RP, Abbas A, Mark GL, O’Gara F (2006) Molecular tools for studying plant growth promoting rhizobacteria (PGPR). In: Cooper and Rao (eds) Molecular techniques for soil and rhizosphere microorganisms. CABI Publishing, Wallingford, Oxfordshire, UK, pp 116–131

  • Frankenberger WT Jr, Dick WA (1983) Relationship between enzyme activities and microbial growth and activity indices in soil. Soil Sci Soc Am J 47:945–951

    Article  CAS  Google Scholar 

  • Gholami A, Shahsavani S, Nezarat S (2009) The effect of plant growth promoting rhizobacteria (PGPR) on germination, seedling growth and yield of maize. Int J Biol Life Sci 1:1

    Google Scholar 

  • Ghosh TK, Saha KC (1997) Effects of inoculation of cyanobacteria on nitrogen status and nutrition of rice (Oryza sativa L.) in an Entisol amended with chemical and organic sources of nitrogen. Biol Fert Soils 24:123–128

    Article  CAS  Google Scholar 

  • Gopalakrishnan S, Humayun P, Kiran BK, Kannan IGK, Vidya MS, Deepthi K, Rupela O (2010) Evaluation of bacteria isolated from rice rhizosphere for biological control of charcoal rot of sorghum caused by Macrophomina phaseolina (Tassi) Goid. World J Microbiol Biotechnol. doi:10.1007/s11274-010-0579-0

  • Green VS, Stott DE, Diack M (2006) Assay of fluorescein diacetate hydrolytic activity-optimization of soil samples. Soil Biol Biochem 38:693–701

    Article  CAS  Google Scholar 

  • Herman MAB, Nault BA, Smart CD (2008) Effects of plant growth promoting rhizobacteria on bell pepper production and green peach aphid infestations in New York. Crop Protect 27:996–1002

    Article  Google Scholar 

  • Jaiswal P, Prasanna R, Nayak S, Sood A, Suseela MR (2008) Characterization of rhizo-cyanobacteria and their associations with wheat seedlings. Egypt J Biol 10:20–27

    Google Scholar 

  • Jaiswal P, Kashyap AK, Prasanna R, Singh PK (2010) Evaluating the potential of N. calcicola and its bicarbonate resistant mutant as bioameliorating agents for ‘Usar’ soil. Ind J Microbiol 50:12–18

    Article  CAS  Google Scholar 

  • Joseph B, Ranjan Patra R, Lawrence R (2007) Characterization of plant growth promoting rhizobacteria associated with chickpea. Int J Plant Prod 1:141–151

    Google Scholar 

  • Kandasamy S, Loganathan K, Muthuraj R, Duraisamy S, Seetharaman S, Thiruvengadam R, Ponnusamy B, Ramasamy S (2009) Understanding the molecular basis of plant growth promotional effect of Psuedomonas fluorescens through protein profiling. Proteome Sci 7:47–54

    Article  Google Scholar 

  • Keyeo F, Ai’shah N, Amir HG (2011) The effect of nitrogen fixing activity and phytohormone production of diazotroph in promoting growth of rice seedlings. Biotechnology 10:267–273

    Article  CAS  Google Scholar 

  • Khalid A, Arshad M, Zahir ZA (2004) Screening plant growth promoting rhizobacteria for improving growth and yield of wheat. J Appl Microbiol 96(3):473–480

    Article  CAS  Google Scholar 

  • Mac Kinney G (1941) Absorption of light by chlorophyll solutions. J Biol Chem 140:315–322

    CAS  Google Scholar 

  • Mader P, Kaiser F, Adholeya A, Singh R, Uppal HS, Sharma AK, Srivastava R, Sahai V, Aragno M, Wiemken A, Johri BN, Fried PM (2011) Inoculation of root microorganisms for sustainable wheat rice and wheat black gram rotations in India. Soil Biol Biochem 43:609–619

    Article  CAS  Google Scholar 

  • Mandal B, Vlek PLG, Mandal LN (1999) Beneficial effects of blue-green algae and Azolla, excluding supplying nitrogen, on wetland rice fields: a review. Biol Fertil Soils 28:329–342

    Article  CAS  Google Scholar 

  • Mehnaz S, Mirza MS, Haurat J, Bally R, Normand P, Bano A, Malik KA (2001) Isolation and 16S rRNA sequence analysis of the beneficial bacteria from the rhizosphere of rice. Can J Microbiol 47:110–117

    Article  CAS  Google Scholar 

  • Minorsky PV (2008) On the inside. Plant Physiol 146:323–324

    Article  CAS  Google Scholar 

  • Morrissey JP, Dow JM, Mark GL, O Gara F (2004) Are microbes at the root of a solution to world food production? Eur Mol Biol Org Rep 5:922–926

    CAS  Google Scholar 

  • Nain L, Rana A, Joshi M, Shrikrishna JD, Kumar D, Shivay YS, Paul S, Prasanna R (2010) Evaluation of synergistic effects of bacterial and cyanobacterial strains as biofertilizers for wheat. Plant Soil 331:217–230

    Article  CAS  Google Scholar 

  • Nayak S, Prasanna R, Pabby A, Dominic TK, Singh PK (2004) Effect of urea and BGA-Azolla biofertilizers on nitrogen and chlorophyll accumulation in soil cores from rice fields. Biol Fertil Soils 40:67–72

    Article  CAS  Google Scholar 

  • Nunan N, Morgan MA, Herlihy M (1998) Ultraviolet absorbance (280 nm) of compounds released from soil during chloroform fumigation as an estimate of the microbial biomass. Soil Biol Biochem 30(12):1599–1603

    Article  CAS  Google Scholar 

  • Piromyou P, Buranabanyat B, Tantasawat P, Tittabutr P, Boonkerd N, Teaumroong N (2011) Effect of plant growth promoting rhizobacteria (PGPR) inoculation on microbial community structure in rhizosphere of forage corn cultivated in Thailand. Eur J Soil Biol 47:44–54

    Article  CAS  Google Scholar 

  • Prasanna R, Tripathi U, Dominic TK, Singh AK, Yadav AK, Singh PK (2003) An improvised technique for measurement of nitrogen fixation by blue-green algae and Azolla using intact soil cores. Exp Agric 39:145–150

    Article  CAS  Google Scholar 

  • Prasanna R, Jaiswal P, Singh YV, Singh PK (2008) Influence of biofertilizers and organic amendments on nitrogenase activity and phototrophic biomass of soil under wheat. Acta Agron Hung 56(2):149–159

    Article  CAS  Google Scholar 

  • Prasanna R, Jaiswal P, Nayak S, Sood A, Kaushik BD (2009a) Cyanobacterial diversity in the rhizosphere of rice and its ecological significance. Ind J Microbiol 49:89–97

    Article  CAS  Google Scholar 

  • Prasanna R, Nain L, Ancha R, Shrikrishna J, Joshi M, Kaushik BD (2009b) Rhizosphere dynamics of inoculated cyanobacteria and their growth-promoting role in rice crop. Egypt J Biol 11:26–36

    Google Scholar 

  • Prasanna R, Joshi M, Rana A, Nain L (2010) Modulation of IAA production in cyanobacteria by tryptophan and light. Polish J Microbiol 59(2):99–105

    CAS  Google Scholar 

  • Rana A, Saharan B, Joshi M, Prasanna R, Kumar K, Nain L (2011) Identification of multi-trait PGPR isolates and evaluating their potential as inoculants for wheat. Ann Microbiol (online first). doi:10.1007/s13213-011-0211-z

  • Rao DLN, Burns RG (1990) The effect of surface growth of blue–green algae and bryophytes on some microbiological, biochemical and physical soil properties. Biol Fertil Soils 9:239–244

    Article  CAS  Google Scholar 

  • Richardson AE, Barea JM, McNeill AM, Prigent-Combaret C (2009) Acquisition of phosphorus and nitrogen in the rhizosphere and plant growth promotion by microorganisms. Plant Soil 32:1305–1339

    Google Scholar 

  • Shende ST, Apte RG, Singh T (1977) Influence of Azotobacter on germination of rice and cotton seeds. Curr Sci 46(19):675–676

    Google Scholar 

  • Smith SE, Read D (2008) Mycorrhizal symbiosis, 3rd edn. Academic Press, London, p 787

    Google Scholar 

  • Subbiah BV, Asija GL (1956) A rapid procedure for the determination of available nitrogen in oils. Curr Sci 25:259–260

    CAS  Google Scholar 

  • Tabatabai MA, Bremner JM (1969) Use of p-nitrophenyl phosphate for assay of soil phosphatase activity. Soil Biol Biochem 1:301–307

    Article  CAS  Google Scholar 

  • Tabatabai MA, Bremner JM (1972) Assay of urease activity in soils. Soil Biol Biochem 4:479–487

    Google Scholar 

  • Vessey JK (2003) Plant growth promoting rhizobacteria as biofertilizers. Plant Soil 255:571–586

    Article  CAS  Google Scholar 

  • Watanabe A, Yamamoto Y (1971) Algal nitrogen fixation in the tropics. Plant Soil special vol: 403–413

  • Yanni YG, Dazzo FB (2010) Enhancement of rice production using endophytic strains of Rhizobium leguminosarum bv. trifolii in extensive field inoculation trials within the Egypt Nile delta. Plant Soil 336:129–142

    Article  CAS  Google Scholar 

  • Yanni YG, Rizk RY, Corich V, Squartini A, Ninke K, Hollingsworth SP, Orgambide G, de Bruijn F, Stoltzfus R, Buckley D, Schmidt TM, Mateos PF, Ladha JK, Dazzo FB (1997) Natural endophytic association between Rhizobium leguminosarum bv. trifolii and rice roots and assessment of its potential to promote rice growth. Plant Soil 194:99–114

    Article  CAS  Google Scholar 

  • Zarrin F, Saleemi M, Zia M, Sultan T, Aslam M, Rehman RU, Chaudhary FM (2009) Antifungal activity of plant growth-promoting rhizobacteria isolates against Rhizoctonia solani in wheat. Afr J Biotechnol 8(2):219–225

    Google Scholar 

Download references

Acknowledgments

The authors are grateful to the Division of Microbiology, Indian Agricultural Research Institute (IARI), New Delhi and Indian Council of Agricultural Research (ICAR-AMAAS) for providing the facilities and financial support, in the form of projects for carrying out the present investigation.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Radha Prasanna.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOC 194 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Prasanna, R., Joshi, M., Rana, A. et al. Influence of co-inoculation of bacteria-cyanobacteria on crop yield and C–N sequestration in soil under rice crop. World J Microbiol Biotechnol 28, 1223–1235 (2012). https://doi.org/10.1007/s11274-011-0926-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11274-011-0926-9

Keywords

Navigation