Abstract
The simultaneous production of hydrogen and ethanol by microorganisms from waste materials in a bioreactor system would establish cost-effective and time-saving biofuel production. This review aims to present the current status of fermentation processes producing hydrogen accompanied by ethanol as a co-product. We outlined the microbes used and their fundamental pathways for hydrogen and ethanol fermentation. Moreover, we discussed the exploitation of renewable and sustainable waste materials as promising feedstock and the limitations encountered. The low substrate bioconversion rate in hydrogen and ethanol co-production is regarded as the primary constraint towards the development of large scale applications. Thus, microbes with an enhanced capability have been generated via genetic manipulation to diminish the inefficiency of substrate consumption. In this review, other potential approaches to improve the performance of co-production through fermentation were also elaborated. This review will be a useful guide for the future development of hydrogen and ethanol co-production using waste materials.
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References
Abdel-Rahman MA, Tashiro Y, Sonomoto K (2013) Recent advances in lactic acid production by microbial fermentation processes. Biotechnol Adv 31:877–902
Agbor V, Zurzolo F, Blunt W, Dartiailh C, Cicek N, Sparling R, Levin DB (2014) Single-step fermentation of agricultural hemp residues for hydrogen and ethanol production. Biomass Bioenergy 64:62–69
Asanuma N, Hino T (2002) Molecular characterization and expression of pyruvate formate-lyase-activating enzyme in a ruminal bacterium, Streptococcus bovis. Appl Environ Microbiol 68:3352–3357
Azbar N, Çetinkaya Dokgöz FT, Keskin T, Korkmaz KS, Syed HM (2009) Continuous fermentative hydrogen production from cheese whey wastewater under thermophilic anaerobic conditions. Int J Hydrog Energy 34:7441–7447
Bakonyi P, Nemestóthy N, Lankó J, Rivera I, Buitrón G, Bélafi-Bakó K (2015) Simultaneous biohydrogen production and purification in a double-membrane bioreactor system. Int J Hydrog Energy 40:1690–1697
Balan V (2014) Current challenges in commercially producing biofuels from lignocellulosic biomass. ISRN Biotechnology 2014:463074. doi:10.1155/2014/463074
Balat M, Balat H (2009) Recent trends in global production and utilization of bio-ethanol fuel. Appl Energy 86:2273–2282
Bang Y, Han SJ, Yoo J, Choi JH, Kang KH, Song JH, Seo JG, Jung JC, Song IK (2013) Hydrogen production by steam reforming of liquefied natural gas (LNG) over trimethylbenzene-assisted ordered mesoporous nickel-alumina catalyst. Int J Hydrog Energy 38:8751–8758
Brodeur G, Yau E, Badal K, Collier J, Ramachandran KB, Ramakrishnan S (2011) Chemical and physicochemical pretreatment of lignocellulosic biomass: a review. Enzyme Res 2011:17. doi:10.4061/2011/787532
Brouwer J (2010) On the role of fuel cells and hydrogen in a more sustainable and renewable energy future. Curr Appl Phys 10:S9–S17
Cai G, Jin B, Monis P, Saint C (2011a) Metabolic flux network and analysis of fermentative hydrogen production. Biotechnol Adv 29:375–387
Cai G, Jin B, Saint C, Monis P (2011b) Genetic manipulation of butyrate formation pathways in Clostridium butyricum. J Biotechnol 155:269–274
Carere CR, Kalia V, Sparling R, Cicek N, Levin DB (2008a) Pyruvate catabolism and hydrogen synthesis pathway genes of Clostridium thermocellum ATCC 27405. Indian J Microbial 48:252–266
Carere CR, Sparling R, Cicek N, Levin DB (2008b) Third generation biofuels via direct cellulose fermentation. Int J Mol Sci 9:1342–1360
Chaubey R, Sahu S, James OO, Maity S (2013) A review on development of industrial processes and emerging techniques for production of hydrogen from renewable and sustainable sources. Renew Sust Energy Rev 23:443–462
Chaudhary N, Ngadi MO, Simpson BK, Kassama LS (2011) Biosynthesis of ethanol and hydrogen by glycerol fermentation using Escherichia coli. Adv Chem Eng Sci 1:83–89
Cheng KK, Zhao XB, Zeng J, Zhang JA (2012) Biotechnological production of succinic acid: current state and perspectives. Biofuels Bioprod Biorefin 6:302–318
Chong ML, Sabaratnam V, Shirai Y, Hassan MA (2009) Biohydrogen production from biomass and industrial wastes by dark fermentation. Int J Hydrog Energy 34:3277–3287
Chookaew T, Sompong O, Prasertsan P (2014) Statistical optimization of medium components affecting simultaneous fermentative hydrogen and ethanol production from crude glycerol by thermotolerant Klebsiella sp. TR17. Int J Hydrog Energy 39:751–760
Chu CY, Sen B, Lay C, Lin Y, Lin C (2012) Direct fermentation of sweet potato to produce maximal hydrogen and ethanol. Appl Energy 100:10–18
Chuang YS, Huang CY, Lay CH, Chen CC, Sen B, Lin CY (2012) Fermentative bioenergy production from distillers grains using mixed microflora. Int J Hydrog Energy 37:15547–15555
Clomburg JM, Gonzalez R (2013) Anaerobic fermentation of glycerol: a platform for renewable fuels and chemicals. Trends Biotechnol 31:20–28
Feng XH, Chen F, Xu H, Wu B, Yao J, Ying HJ, Ouyang PK (2010) Propionic acid fermentation by Propionibacterium freudenreichii CCTCC M207015 in a multi-point fibrous-bed bioreactor. Bioproc Biosyst Eng 33:1077–1085
Ferreira Rosa PR, Santos SC, Silva EL (2014) Different ratios of carbon sources in the fermentation of cheese whey and glucose as substrates for hydrogen and ethanol production in continuous reactors. Int J Hydrog Energy 39:1288–1296
Förster AH, Gescher J (2014) Metabolic engineering of Escherichia coli for production of mixed-acid fermentation end products. Front Bioeng Biotechnol 2:16. doi:10.3389/fbioe.2014.00016
Frigon JC, Guiot SR (2010) Biomethane production from starch and lignocellulosic crops: a comparative review. Biofuels Bioprod Biorefin 4:447–458
Garrett RH, Grisham CM (2013) Biochemistry. California, Belmont
Guo WQ, Ren NQ, Wang XJ, Xiang WS, Meng ZH, Ding J, Qu YY, Zhang LS (2008) Biohydrogen production from ethanol-type fermentation of molasses in an expanded granular sludge bed (EGSB) reactor. Int J Hydrog Energy 33:4981–4988
Han W, Wang Z, Chen H, Yao X, Li Y (2011) Simultaneous biohydrogen and bioethanol production from anaerobic fermentation with immobilized sludge. J Biomed Biotechnol 2011:343791. doi:10.1155/2011/343791
Han W, Chen H, Jiao A, Wang Z, Li Y, Ren N (2012) Biological fermentative hydrogen and ethanol production using continuous stirred tank reactor. Int J Hydrog Energy 37:843–847
Ho CY, Chang JJ, Lin JJ, Chin TY, Mathew GM, Huang CC (2011) Establishment of functional rumen bacterial consortia (FRBC) for simultaneous biohydrogen and bioethanol production from lignocellulose. Int J Hydrog Energy 36:12168–12176
Hu H, Wood TK (2010) An evolved Escherichia coli strain for producing hydrogen and ethanol from glycerol. Biochem Biophys Res Commun 391:1033–1038
Huang J, Dincer I (2014) Parametric analysis and assessment of a coal gasification plant for hydrogen production. Int J Hydrog Energy 39:3294–3303
Huang H, Liu H, Gan YR (2010) Genetic modification of critical enzymes and involved genes in butanol biosynthesis from biomass. Biotechnol Adv 28:651–657
Hwang MH, Jang NJ, Hyun SH, Kim ISJ (2004) Anaerobic biohydrogen production from ethanol fermentation: the role of pH. J Biotechnol 111:297–309
Intanoo P, Suttikul T, Leethochawalit M, Gulari E, Chavadej S (2014) Hydrogen production from alcohol wastewater with added fermentation residue by an anaerobic sequencing batch reactor (ASBR) under thermophilic operation. Int J Hydrog Energy 39:9611–9620
Ito T, Nakashimada Y, Senba K, Matsui T, Nishio N (2005) Hydrogen and ethanol production from glycerol-containing wastes discharged after biodiesel manufacturing process. J Biosci Bioeng 100:260–265
Ji XJ, Huang H, Du J, Zhu JG, Ren LJ, Hu N, Li S (2009) Enhanced 2,3-butanediol production by Klebsiella oxytoca using a two-stage agitation speed control strategy. Bioresour Technol 100:3410–3414
Jitrwung R, Yargeau V (2011) Optimization of media composition for the production of biohydrogen from waste glycerol. Int J Hydrog Energy 36:9602–9611
Jo JH, Jeon CO, Lee SY, Lee DS, Park JM (2010) Molecular characterization and homologous overexpression of [FeFe]-hydrogenase in Clostridium tyrobutyricum JM1. Int J Hydrog Energy 35:1065–1073
Jones DT, Woods DR (1986) Acetone-butanol fermentation revisited. Microbiol Rev 50:484–524
Kapdan IK, Kargi F (2006) Bio-hydrogen production from waste materials. Enzyme Microb Technol 38:569–582
Khanna N, Dasgupta CN, Mishra P, Das D (2011) Homologous overexpression of [FeFe] hydrogenase in Enterobacter cloacae IIT-BT 08 to enhance hydrogen gas production from cheese whey. Int J Hydrog Energy 36:15573–15582
Koskinen PE, Beck SR, Orlygsson J, Puhakka JA (2008) Ethanol and hydrogen production by two thermophilic, anaerobic bacteria isolated from Icelandic geothermal areas. Biotechnol Bioeng 101:679–690
Kotay SM, Das D (2009) Novel dark fermentation involving bioaugmentation with constructed bacterial consortium for enhanced biohydrogen production from pretreated sewage sludge. Int J Hydrog Energy 34:7489–7496
Kuchenreuther JM, Grady-Smith CS, Bingham AS, George SJ, Cramer SP, Swartz JR (2010) High-yield expression of heterologous [FeFe] hydrogenase in Escherichia coli. PLoS ONE 5:e15491. doi:10.1371/journal.pone.0015491
Kumar P, Barrett DM, Delwiche MJ, Stroeve P (2009) Methods for pretreatment of lignocellulosic biomass for efficient hydrolysis and biofuel production. Ind Eng Chem Res 48:3713–3729
Lam MK, Lee KT (2010) Renewable and sustainable bioenergies production from palm oil mill effluent (POME): win-win strategies toward better environmental protection. Biotechnol Adv 29:124–141
Lambertz C, Garvey M, Klinger J, Heesel D, Klose H, Fischer R, Commandeur U (2014) Challenges and advances in the heterologous expression of cellulolytic enzymes: a review. Biotechnol Biofuels 7:135. doi:10.1186/s13068-014-0135-5
Lay CH, Lin HC, Sen B, Chu CY, Lin CY (2012) Simultaneous hydrogen and ethanol production from sweet potato via dark fermentation. J Clean Prod 27:155–164
Lay CH, Sen B, Huang SC, Chen CC (2013) Sustainable bioenergy production from tofu-processing wastewater by anaerobic hydrogen fermentation for onsite energy recovery. Renew Energy 58:60–67
Lee SY, Park JH, Jang SH, Nielsen LK, Kim J, Jung KS (2008) Fermentative butanol production by Clostridia. Biotechnol Bioeng 101:209–228
Lehmann D, Lütke-Eversloh T (2011) Switching Clostridium acetobutylicum to an ethanol producer by disruption of the butyrate/butanol fermentative pathway. Metab Eng 13:464–473
Levin DB, Chahine R (2009) Challenges for renewable hydrogen production from biomass. Int J Hydrog Energy 35:4962–4969
Lin MY, Hourng LW, Kuo CW (2012) The effect of magnetic force on hydrogen production efficiency in water electrolysis. Int J Hydrog Energy 37:1311–1320
Maeda T, Sanchez-Torres V, Wood TK (2012) Hydrogen production by recombinant Escherichia coli strains. Microb Biotechnol 5:214–225
Manikkandan TR, Thirumavalavan K, Ravi R, Dhanasekar R (2013) Simultaneous production of hydrogen and ethanol from sugarcane bagasse using newly isolated strain. Int J Env Bioenergy 5:136–148
Manish S, Venkatesh K, Banerjee R (2007) Metabolic flux analysis of biological hydrogen production by Escherichia coli. Int J Hydrog Energy 32:3820–3830
Maru BT, Constanti M, Stchigel AM, Medina F, Sueiras JE (2012) Biohydrogen production by dark fermentation of glycerol using Enterobacter and Citrobacter sp. Biotechnol Prog 29:31–38
Masset J, Calusinska M, Hamilton C, Hiligsmann S, Joris B, Wilmotte A, Thonart P (2012) Fermentative hydrogen production from glucose and starch using pure strains and artificial co-cultures of Clostridium spp. Biotechnol Biofuels 5:35. doi:10.1186/1754-6834-5-35
Mekhilef S, Saidur R, Safari A, Mustaffa WESB (2011) Biomass energy in Malaysia: current state and prospects. Renew Sust Energy Rev 15:3360–3370
Murarka A, Dharmadi Y, Shams Yazdani S, Gonzalez R (2008) Fermentative utilization of glycerol by Escherichia coli and its implications for the production of fuels and chemicals. Appl Env Microbiol 74:1124–1135
Naik SN, Goud VV, Rout PK, Dalai AK (2010) Production of first and second generation biofuels: a comprehensive review. Renew Sust Energy Rev 14:578–597
Ntaikou I, Antonopoulou G, Lyberatos G (2010) Biohydrogen production from biomass and wastes via dark fermentation: a review. Waste Biomass Valor 1:21–39
Nwachukwu R, Shahbazi A, Wang L, Ibrahim S, Worku M, Schimmel K (2012) Bioconversion of glycerol to ethanol by a mutant Enterobacter aerogenes. AMB Express 2:20. doi:10.1186/2191-0855-2-20
Orlygsson J, Sigurbjornsdottir MA, Bakken HE (2010) Bioprospecting thermophilic ethanol and hydrogen producing bacteria from hot springs in Iceland. Icel Agric Sci 23:73–85
Papoutsakis ET (2008) Engineering solventogenic clostridia. Curr Opin Biotechnol 19:420–429
Pinilla JL, Utrilla R, Karn RK, Suelves I, Lázaro MJ, Moliner R, García AB, Rouzaud JN (2011) High temperature iron-based catalysts for hydrogen and nanostructured carbon production by methane decomposition. Int J Hydrog Energy 36:7832–7843
Rachman MA, Furutani Y, Nakashimada Y, Kakizono T, Nishio N (1997) Enhanced hydrogen production in altered mixed acid fermentation of glucose by Enterobacter aerogenes. J Ferment Bioeng 83:358–363
Ramachandran U, Wrana N, Cicek N, Sparling R, Levin DB (2011) Isolation and characterization of a hydrogen- and ethanol-producing Clostridium sp. strain URNW. Can J Microbiol 57:236–243
Rana V, Eckard AD, Ahring BK (2014) Comparison of SHF and SSF of wet exploded corn stover and loblolly pine using in-house enzymes produced from T. reesei RUT C30 and A. saccharolyticus. Springerplus 3:516. doi:10.1186/2193-1801-3-516
Ren NQ, Guo WQ, Wang XJ, Xiang WS, Liu BF, Wang XZ, Ding J, Chen ZB (2008) Effects of different pretreatment methods on fermentation types and dominant bacteria for hydrogen production. Int J Hydrog Energy 33:4318–4324
Reungsang A, Sittijunda S, Angelidaki I (2013) Simultaneous production of hydrogen and ethanol from waste glycerol by Enterobacter aerogenes KKU-S1. Int J Hydrog Energy 38:1813–1825
Romero-Garcia S, Hernández-Bustos C, Merino E, Gosset G, Martinez A (2009) Homolactic fermentation from glucose and cellobiose using Bacillus subtilis. Microb Cell Fact 8:23. doi:10.1186/1475-2859-8-23
Rosales-Colunga LM, Alvarado-Cuevas ZD, Razo-Flores E, Rodríguez Ade L (2013) Maximizing hydrogen production and substrate consumption by Escherichia coli WDHL in cheese whey fermentation. Appl Biochem Biotechnol 171:704–715
Saint-Amans S, Girbal L, Andrade J, Ahrens K, Soucaille P (2001) Regulation of carbon and electron flow in Clostridium butyricum VPI 3266 grown on glucose-glycerol mixtures. J Bacteriol 183:1748–1754
Sakai S, Yagishita T (2007) Microbial production of hydrogen and ethanol from glycerol-containing wastes discharged from a biodiesel fuel production plant in a bioelectrochemical reactor with thionine. Biotechnol Bioeng 98:340–348
Shams Yazdani S, Gonzalez R (2008) Engineering Escherichia coli for the efficient conversion of glycerol to ethanol and co-products. Metab Eng 10:340–351
Sigurbjornsdottir MA, Orlygsson J (2012) Combined hydrogen and ethanol production from sugars and lignocellulosic biomass by Thermoanaerobacterium AK54, isolated from hot spring. Appl Energy 97:785–791
Sivagurunathan P, Kumar G, Lin CY (2014) Hydrogen and ethanol fermentation of various carbon sources by immobilized Escherichia coli (XL1-Blue). Int J Hydrog Energy 39:6881–6888
Song H, Lee SY (2006) Production of succinic acid by bacterial fermentation. Enzyme Microb Tech 39:352–361
Suhaimi SN, Phang LY, Maeda T, Abd-Aziz S, Wakisaka M, Shirai Y, Hassan MA (2012) Conversion of glycerol for bioethanol production using isolated Escherichia coli SS1. Braz J Microbiol 43:506–516
Talebian-Kiakalaieh A, Saidina Amin NA, Mazaheri H (2013) A review on novel processes of biodiesel production from waste cooking oil. Appl Energy 104:683–710
Tashiro Y, Sonomoto K (2010) Advances in butanol production by clostridia. In: Méndez-Vilas A (ed) Current research, technology and education topics in applied microbiology and microbial biotechnology. Formatex, Spain, pp 1383–1394
Varrone C, Giussani B, Izzo G, Massini G, Marone A, Signorini A, Wang A (2012) Statistical optimization of biohydrogen and ethanol production from crude glycerol by microbial mixed culture. Int J Hydrog Energy 37:16479–16488
Wang X, Jin B (2009) Process optimization of biological hydrogen production from molasses by a newly isolated Clostridium butyricum W5. J Biosci Bioeng 107:138–144
Wu KJ, Lin YH, Lo YC, Chen CY, Chen WM, Chang JS (2011) Converting glycerol into hydrogen, ethanol, and diols with a Klebsiella sp. HE1 strain via anaerobic fermentation. J Taiwan Inst Chem Eng 42:20–25
Yokoi H, Saitsu A, Uchida H, Hirose J, Hayashi S, Takasaki Y (2001) Microbial hydrogen production from sweet potato starch residue. J Biosci Bioeng 91:58–63
Yoshida A, Nishimura T, Kawaguchi H, Inui M, Yukawa H (2006) Enhanced hydrogen production from glucose using ldh- and frd-inactivated Escherichia coli strains. Appl Microbiol Biotechnol 73:67–72
Zhang YHP (2011) What is vital (and not vital) to advance economically-competitive biofuels production. Process Biochem 46:2091–2110
Zhang C, Yang H, Yang F, Ma Y (2009) Current progress on butyric acid production by fermentation. Curr Microbiol 59:656–663
Zhao C, Sompong O, Karakashev D, Angelidaki I, Lu W, Wang H (2009) High yield simultaneous hydrogen and ethanol production under extreme-thermophilic (70 & #xB0;C) mixed culture environment. Int J Hydrog Energy 34:5657–5665
Zhao C, Karakashev D, Lu W, Wang H, Angelidaki I (2010) Xylose fermentation to biofuels (hydrogen and ethanol) by extreme thermophilic (70 & #xB0;C) mixed culture. Int J Hydrog Energy 35:3415–3422
Zhao L, Cao GL, Wang AJ, Guo WQ, Ren HY, Ren NQ (2013) Simultaneous saccharification and fermentation of fungal pretreated cornstalk for hydrogen production using Thermoanaerobacterium thermosaccharolyticum W16. Bioresour Technol 145:103–107
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The authors would like to acknowledge the Fundamental Research Grant Scheme (FRGS/1/2012/SG06/UCSI/02/1) for funding the project.
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Soo, CS., Yap, WS., Hon, WM. et al. Mini review: hydrogen and ethanol co-production from waste materials via microbial fermentation. World J Microbiol Biotechnol 31, 1475–1488 (2015). https://doi.org/10.1007/s11274-015-1902-6
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DOI: https://doi.org/10.1007/s11274-015-1902-6