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In Vivo Imaging of Sentinel Nodes Using Fluorescent Silica Nanoparticles in Living Mice

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Abstract

Purpose

We examine the feasibility of fluorescent imaging system for sentinel lymph node detection by using functionalized silica nanoparticles.

Materials and Methods

We developed a functionalized RITC-SiO2 nanoparticles containing fluorescent dye, C28H31N2O3Cl (rhodamine B isothiocyanate) inside, and subsequently synthesized 68Ga-NOTA-RITC-SiO2 nanoparticles.

Results

At 5 min after RITC-doped silica nanoparticles injection, fluorescent signals were shown in both right axillary lymph node (ALN) and injection site of living mice. Fluorescent signals were also observed at these locations in a biodistribution study. In addition, fluorescence was detected in frozen ALN sections microscopically. The percentages of doses injected per gram of tissue of axillary and brachial lymph nodes near footpad treated with 68Ga-NOTA-RITC-SiO2 nanoparticles were 308.3 ± 3.4 and 41.5 ± 6.1, respectively. Little 68Ga radioactivity was found in other organs.

Conclusion

Our data provide strong evidence that functionalized silica nanoparticles has a promising potential as organic lymphatic tracer in biomedical imaging such as pre- and intraoperative surgical guidance.

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Acknowledgments

This work was supported by the Interdisciplinary Research Initiatives Program of the College of Natural Sciences and the College of Medicine, Seoul National University 2008 and by Research Division of Human Life Science of the second stage Brain Korea 21 Project in 2009.

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Authors and Affiliations

  1. Department of Nuclear Medicine, Seoul National University College of Medicine, 28 Yongon-dong, Jongno-gu, Seoul, 110-744, South Korea

    Yong Hyun Jeon, Yun-Sang Lee, Jae Min Jeong, June-Key Chung, Dong Soo Lee, Myung Chul Lee & Keon Wook Kang

  2. Department of Tumor Biology, Seoul National University College of Medicine, Seoul, South Korea

    Yong Hyun Jeon, June-Key Chung & Keon Wook Kang

  3. Department of Laboratory of Molecular Imaging and Therapy of Cancer Research Institute, Seoul National University, Seoul, South Korea

    Yong Hyun Jeon, Young-Hwa Kim, June-Key Chung & Keon Wook Kang

  4. Department of Chemistry, Seoul National University, Seoul, 151-747, South Korea

    Kihwan Choi, Jing Yu Piao, Bo Quan & Doo Soo Chung

  5. Department of Nuclear Medicine, School of Medicine, Kyungpook National University, Daegu, South Korea

    Yong Hyun Jeon & Jaetae Lee

  6. Department of Nuclear Medicine and Biomedical Sciences, Seoul National University College of Medicine, 28 Yongon-dong, Jongno-gu, Seoul, 110-744, South Korea

    Young-Hwa Kim

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  1. Yong Hyun Jeon
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Corresponding authors

Correspondence to Doo Soo Chung or Keon Wook Kang.

Additional information

Yong Hyun Jeon and Young-Hwa Kim contributed equally to this work.

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Table S1

Biodistribution of 68Ga-NOTA-RITC-SiO2 nanoparticles in nude mice. The %ID/g values of axillary and brachial lymph nodes near footpad treated with 68Ga-NOTA-RITC-SiO2 nanoparticles were 308.3 ± 3.4 and 41.5 ± 6.1, respectively. Little 68Ga radioactivity was found in other organs. (e.g., liver, lung, brain, spleen, and kidney) ALN axillary lymph node, IN inguinal lymph node, SN sciatic lymph node, BLN brachial lymph node, SCN superficial cervical lymph node. Data are expressed as %ID/g of tissue. n = 5 mice (PDF 482 KB)

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Jeon, Y.H., Kim, YH., Choi, K. et al. In Vivo Imaging of Sentinel Nodes Using Fluorescent Silica Nanoparticles in Living Mice. Mol Imaging Biol 12, 155–162 (2010). https://doi.org/10.1007/s11307-009-0262-8

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  • DOI: https://doi.org/10.1007/s11307-009-0262-8

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