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Green synthesis of silver nanoparticles by microorganism using organic pollutant: its antimicrobial and catalytic application

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Abstract

A novel approach for the green synthesis of silver nanoparticles (AgNPs) from aqueous solution of AgNO3 using culture supernatant of phenol degraded broth is reported in this work. The synthesis was observed within 10 h, and AgNPs showed characteristic surface plasmon resonance around 410 nm. Spherical nanoparticles of size less than 30 nm were observed in transmission electron microscopy. X-ray diffraction pattern corresponding to 111, 200, 220, and 311 revealed the crystalline nature of the as-formed nanoparticles. It was found that the colloidal solution of AgNP suspensions exhibited excellent stability over a wide range of ionic strength, pH, and temperature. The effect of pH and ionic strength indicated that stabilization is due to electrostatic repulsion arising from the negative charge of the conjugate proteins. The AgNPs showed highly potent antimicrobial activity against Gram-positive, Gram-negative, and fungal microorganisms. The as-prepared AgNPs showed excellent catalytic activity in reduction of 4-nitrophenol to 4-aminophenol by NaBH4. By manufacturing magnetic alginate beads, the reusability of the AgNPs for the catalytic reaction has been demonstrated.

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Acknowledgments

The authors are very grateful to Board of Research in Nuclear Sciences (BRNS) and Department of Science and Technology (DST), Delhi for providing the funds to carry out the research activities. Also, the authors are thankful to SAIF IIT-Bombay, Mumbai for TEM facility. The authors are very grateful to Department of Microbiology, Biochemistry and Biotechnology, Shivaji University, Kolhapur for allowing the use of facilities to carry out the experiments.

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Authors and Affiliations

  1. Center for Interdisciplinary Research, D. Y. Patil University, Kolhapur, 416 006, Maharashtra State, India

    S. V. Otari, R. M. Patil, S. J. Ghosh & S. H. Pawar

  2. Department of Microbiology, Shivaji University, Kolhapur, 416 004, Maharashtra State, India

    N. H. Nadaf

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  1. S. V. Otari
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  2. R. M. Patil
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  3. N. H. Nadaf
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  4. S. J. Ghosh
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  5. S. H. Pawar
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Correspondence to S. H. Pawar.

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Responsible editor: Philippe Garrigues

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Otari, S.V., Patil, R.M., Nadaf, N.H. et al. Green synthesis of silver nanoparticles by microorganism using organic pollutant: its antimicrobial and catalytic application. Environ Sci Pollut Res 21, 1503–1513 (2014). https://doi.org/10.1007/s11356-013-1764-0

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  • DOI: https://doi.org/10.1007/s11356-013-1764-0

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