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Spatiotemporal dynamics of bacterial and archaeal communities in household biogas digesters from tropical and subtropical regions of Yunnan Province, China

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Abstract

A combination of 16S rRNA gene PCR-based techniques and the determination of abiotic factors were used to study community composition, richness, and evenness and the correlation between biotic and abiotic factors in 19 household biogas digesters in tropical and subtropical regions of Yunnan Province, China. The results revealed that both bacterial and archaeal community composition differed between regions and archaeal community composition was more affected by season than bacterial; regardless of sampling location, the dominant bacterial phyla included Chloroflexi, Bacteroidetes, Firmicutes, and Proteobacteria, and the most dominant archaeal phylum was Euryarchaeota; in digesters from both regions, Chloroflexi as the first or second most dominant bacteria accounted for 21.50–26.10 % of bacterial library sequences, and the phylum Crenarchaeota as the second most dominant archaea accounted for 17.65–19.77 % of archaeal library sequences; the species Methanosaeta concilii as the most dominant archaeal species accounted for 67.80–72.80 % of the sequences. This study found that most of the abundant microbial communities in 19 biogas digesters are similar, and this result will provide enlightenment for finding the universal nature in rural biogas digesters at tropical and subtropical regions in China.

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Acknowledgments

We are thankful for grants from the National Natural Science Foundation of China (NSFC; 31160123/C0309), the Yunnan Provincial Sciences and Technology Platform Promotion Plan (2013DH041), the Specialized Research Fund for Doctoral Program of Universities (20135303110001), Yunnan Province Key Fund of Applied Basic Research (2014FA030) and Open Fund from Yunnan Key Laboratory of Rural Energy Engineering (2015KF07). We are also grateful to the staff of the forestry bureaus of XishuangBanna Autonomous Region and Jianshui County.

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    1. Yunnan Research Center of Biogas Technology and Engineering, Yunnan Normal University, Kunming, 650500, People’s Republic of China

      Guangliang Tian, Qiumin Li, Bin Yang, Lijuan Zhang, Yingjuan Li, Fang Yin, Xingling Zhao & Wudi Zhang

    2. Engineering and Research Center of Sustainable Development and Utilization of Bioenergy, Ministry of Education, Yunnan Normal University, Kunming, 650500, People’s Republic of China

      Guangliang Tian, Qiumin Li, Bin Yang, Lijuan Zhang, Yingjuan Li, Fang Yin, Xingling Zhao & Wudi Zhang

    3. Yunnan Key Laboratory of Rural Energy Engineering, Yunnan Normal University, Kunming, 650500, People’s Republic of China

      Guangliang Tian, Qiumin Li, Bin Yang, Lijuan Zhang, Yingjuan Li, Fang Yin, Xingling Zhao & Wudi Zhang

    4. Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

      Minghua Dong, Yan Wu, Yongxia Wang, Wei Xiao & Xiaolong Cui

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    1. Guangliang Tian
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    Guangliang Tian and Qiumin Li contributed equally to this work.

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    Tian, G., Li, Q., Dong, M. et al. Spatiotemporal dynamics of bacterial and archaeal communities in household biogas digesters from tropical and subtropical regions of Yunnan Province, China. Environ Sci Pollut Res 23, 11137–11148 (2016). https://doi.org/10.1007/s11356-016-6265-5

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