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Bioremediation treatment improves water quality for Nile tilapia (Oreochromis niloticus) under crude oil pollution

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Abstract

Despite favorable publicity of bioremediation as an affordable technology for cleanup of crude oil, public concerns on ecological safety in the presence of residual oil remain a global challenge. In this study, effects of crude oil exposure at sublethal concentration (0.25% v/v) and bioremediation treatment were investigated on histology and biochemical parameters of organs (gills, liver, kidney, and brain) of juvenile Nile tilapia (Oreochromis niloticus). Ten percent (10%) of mixed bacterial culture was used for bioaugmentation treatment. Ninety juvenile fish were used for study, and experiments were carried out in triplicates for three different groups. Malondialdehyde (MDA), an index of lipid peroxidation, was assayed as biomarker for oxidative stress. Activities of antioxidant enzymes (superoxide dismutase (SOD) and catalase (CAT)), level of non-enzymatic antioxidant (reduced glutathione (GSH)), and activity of brain acetylcholinesterase (AChE) were assayed in selected fish organs as markers for environmental stressor. Histological examination of fish organs was done for all groups. Results of treated groups were compared with those of the control. Levels of MDA significantly increased with significant inhibition of antioxidant enzyme activities in the polluted group. Activities of SOD, CAT, and AChE and levels of GSH in fish organs in the bioaugmentation group were similar to results obtained in the control. Remarkably, the bioaugmentation group showed minimal or no lesions which suggested the efficacy of bioremediation technique in alleviating crude oil toxicity and preserving normal physiology of fish. This study provides deeper insights into the ecosafety of bioremediation treatment and can be extrapolated to other species of fish.

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Funding

This study received financial support from the TETFund, Nigeria, through the Institutional Based Research Grant (IBR) 2015.

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Authors and Affiliations

  1. Enzyme Biotechnology and Environmental Health Unit, Department of Biochemistry, School of Sciences, Federal University of Technology, Akure, Ondo State, 340252, Nigeria

    Folasade M. Olajuyigbe, Oluwaseun A. Adeleye, Ayodele O. Kolawole, Tolulope O. Bolarinwa & Joshua O. Ajele

  2. Department of Fisheries and Aquaculture Technology, School of Agriculture and Agricultural Technology, Federal University of Technology, Akure, Nigeria

    Emmanuel A. Fasakin

  3. Department of Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Benin, Benin City, Nigeria

    Ebunoluwa R. Asenuga

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  1. Folasade M. Olajuyigbe
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  2. Oluwaseun A. Adeleye
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  4. Tolulope O. Bolarinwa
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Correspondence to Folasade M. Olajuyigbe.

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Olajuyigbe, F.M., Adeleye, O.A., Kolawole, A.O. et al. Bioremediation treatment improves water quality for Nile tilapia (Oreochromis niloticus) under crude oil pollution. Environ Sci Pollut Res 27, 25689–25702 (2020). https://doi.org/10.1007/s11356-020-09020-8

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