Abstract
We investigated the occurrence of 14 selected antibiotics including five therapeutic classes of tetracyclines, sulfonamides, macrolides, fluoroquinolones and chloramphenicols in manures collected from four swine farms of different sizes in eastern China. Tetracyclines (tetracycline, oxytetracycline, chlortetracycline, and doxycycline) and sulfadiazine were the most prominent contaminants in the manure samples, with maximum concentrations reaching 98.2 × 103, 354.0 × 103, 139.4 × 103, 37.2× 103, and 7.1× 103 μg/kg, respectively. The occurrence of these compounds was dependent on breeding scale, animal type, and breeding season. Manure storage and vermiculture were not able to effectively deplete the heavier contaminants (tetracyclines and sulfadiazine), resulting in high levels of these chemicals remaining in manures. Therefore, the occurrence of these antibiotics in agricultural soils (0.1–205.1 μg/kg) collected from four types of agricultural land (pear orchard, tea plantation, bamboo forest, and paddy field) near the studied farms, was a reflection of manure application. However, the extremely high concentrations of antibiotics in manures were unlikely from feed consumption, but from other direct forms of medicine application.
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Boxall A B A, Kolpin D W, Halling-Sørensen B, et al. Are veterinary medicines causing environmental risk? Environ Sci Technol, 2003, 37: 287A–294A
Sarmah A K, Meyer M T, Boxall A B A. A global perspective on the use, sales, exposure pathways, occurrence, fate and effects of veterinary antibiotics (VAs) in the environment. Chemosphere, 2006, 65: 725–759
Bound J P, Voulvoulis N. Pharmaceuticals in the aquatic environment — A comparison of risk assessment strategies. Chemosphere, 2004, 56: 1143–1155
Campagnolo E R, Johnson K R, Karpati A, et al. Antimicrobial residues in animal waste and water resources proximal to large-scale swine and poultry feeding operations. Sci Total Environ, 2002, 299: 89–95
Batt A L, Snow D D, Aga D S. Occurrence of sulfonamide antimicrobials in private water wells in Washington County, Idaho, USA. Chemosphere, 2006, 64: 1963–1971
Matsui Y, Ozub T, Inouec T, et al. Occurrence of a veterinary antibiotic in streams in a small catchment area with livestock farms. Desalination, 2008, 226: 215–221
Hu X G, Zhou Q X, Luo Y. Occurrence and source analysis of typical veterinary antibiotics in manure, soil, vegetables and groundwater from organic vegetable bases, northern China. Environ Pollut, 2010, 158: 2992–2998
Zhao L, Dong Y H, Wang H. Residues of veterinary antibiotics in manures from feedlot livestock in eight provinces of China. Sci Total Environ, 2010, 408: 1069–1075
Kemper N. Veterinary antibiotics in the aquatic and terrestrial environment: A review. Ecol Indic, 2008, 8: 1–13
Morley N J. Environmental risk and toxicology of human and veterinary waste pharmaceutical exposure to wild aquatic host-parasite relationships. Environ Toxicol Pharmacol, 2009, 27: 161–175
Ben W W, Qiang Z M, Pan X, et al. Removal of veterinary antibiotics from sequencing batch reactor (SBR) pretreated swine wastewater by Fenton’s reagent. Water Res, 2009, 43: 4392–4402
Hu X G, Yi L, Zhou Q X, et al. Determination of thirteen antibiotics residues in manure by solid phase extraction and high performance liquid Chromatography. Chinese J Anal Chem, 2008, 36: 1162–1166
Xian Q M, Hu L X, Chen H C, et al. Removal of nutrients and veterinary antibiotics from swine wastewater by a constructed macrophyte floating bed system. J Environ Manage, 2010, 91: 2657–2661
Zhang H M, Zhang M K, Gu G P. Residues of tetracyclines in livestock and poultry manures and agricultural soils from north Zhejiang Province (in Chinese). J Eco Rural Environ, 2008, 24: 69–73
Chang X S, Meyer M T, Liu X Y, et al. Determination of antibiotics in sewage from hospitals, nursery and slaughter house, wastewater treatment plant and source water in Chongqing region of Three Gorge Reservoir in China. Environ Pollut, 2010, 158: 1444–1450
Bernal M P, Alburquerque J A, Moral R. Composting of animal manures and chemical criteria for compost maturity assessment: A review. Bioresour Technol, 2009, 100: 5444–5453
Dolliver H, Gupta S, Noll S. Antibiotic degradation during manure composting. J Environ Qual, 2008, 37: 1245–1253
Boxall A B A. Fate of veterinary medicines applied to soils. In: Kümmerer K, ed. Pharmaceuticals in the Environment. Berlin Heidelberg: Springer, 2008. 103–119
National Bureau of Statistics of China. China Statistical Yearbook 2009. http://www.stats.gov.cn/tjsj/ndsj/
Jin X C, Xu Q J, Huang C Z. Current status and future tendency of lake eutrophication in China. Sci China Ser C-Life Sci, 2005, 48: 948–954
Guo L. Doing battle with the green monster of Taihu Lake. Science, 2007, 317: 1166
Mcardell C S, Molnar E, Suter M J, et al. Occurrence and fate of macrolide antibiotics in wastewater treatment plants and in the Glatt Valley watershed, Switzerland. Environ Sci Technol, 2003, 37: 5479–5486
Li Y W, Mo C H, Zhao N, et al. Determination of sulfonamides antibiotics in water and soil using high performance liquid chromatography (in Chinese). Chinese J Anal Chem, 2008, 36: 954–958
Blackwell P A, Holten Lützhøft H C, Ma H P, et al. Ultrasonic extraction of veterinary antibiotics from soils and pig slurry with SPE clean-up and LC-UV and fluorescence detection. Talanta, 2004, 64: 1058–1064
Martínez-Carballo E, González-Barreiro C, Scharf S, et al. Environmental monitoring study of selected veterinary antibiotics in animal manure and soils in Austria. Environ Pollut, 2007, 148: 570–579
Karcı A, Balcıoğlu I A. Investigation of the tetracycline, sulfonamide, and fluoroquinolone antimicrobial compounds in animal manure and agricultural soils in Turkey. Sci Total Environ, 2009, 407: 4652–4664
Guo B. The study on occurrence and the environmental risk of antibiotics in animal waste applied to land (in Chinese). Master Dissertation. Guangzhou: Jinan University, 2009. 29
Zhang S Q. Analysis of harmful and components and harmless processing of livestock and poultry manures (in Chinese). Dissertation for Postdoctoral. Beijing: Graduate School of Chinese Academy of Agricultural Sciences, 2004. 11
Zhang H M, Zhang M K, Gu G P. Residues of tetracyclines in livestock and poultry manures and agricultural soils from north Zhejiang Province (in Chinese). J Ecol Rural Environ, 2008, 24: 69–73
Kobayashi T, Suehiro F, Tuyen B C, et al. Distribution and diversity of tetracycline resistance genes encoding ribosomal protection proteins in Mekong river sediments in Vietnam. FEMS Microbiol Ecol, 2007, 59: 729–737
Zhang X X, Zhang T, Fang H H. Antibiotic resistance genes in water environment. Appl Microbiol Biotechnol, 2009, 82: 397–414
Ben W W, Qiang Z M, Adams C, et al. Simultaneous determination of sulfonamides, tetracyclines and tiamulin in swine wastewater by solid-phase extraction and liquid chromatography-mass spectrometry. J Chromatogr A, 2008, 1202: 173–180
Tong L, Li P, Wang Y X, et al. Analysis of veterinary antibiotics residues in swine wastewater and environmental water samples using optimized SPE-LC/Ms/Ms. Chemosphere, 2009, 74: 1090–1097
Chen Y S, Zhang H B, Luo Y M, et al. A preliminary study on the occurrence and dissipation of antibiotics in swine wastewater (in Chinese). Acta Sci Circumstantiae, 2010, 30: 2205–2212
Christiana T, Schneidera R J, Färberb H A, et al. Determination of antibiotic residues in manure, soil and surface waters. Acta Hydrochim Hydrobiol, 2003, 31: 36–44
Jacobsen A M, Halling-Sørensen B. Multi-component analysis of tetracyclines, sulfonamides and tylosin in swine manure by liquid chromatography-tandem mass spectrometry. Anal Bioanal Chem, 2006, 384: 1164–1174
Aust M O, Godlinski F, Travis G R, et al. Distribution of sulfamethazine, chlortetracycline and tylosin in manure and soil of Canadian feedlots after subtherapeutic use in cattle. Environ Pollut, 2008, 156: 1243–1251
Xu W H, Zhang G, Zou S C, et al. Determination of selected antibiotics in the Victoria Harbour and the Pearl River, South China using high-performance liquid chromatography-electrospray ioization tandem mass spectrometry. Environ Pollut, 2007, 145: 672–679
Winckler C, Grafe A. Use of veterinary drugs in intensive animal production: Evidence for persistence of tetracycline in pig slurry. J Soil Sediment, 2001, 1: 66–70
Arikan O A, Mulbry W, Rice C. Management of antibiotic residues from agricultural sources: Use of composting to reduce chlortetracycline residues in beef manure from treated animals. J Hazard Mater, 2006, 164: 483–489
Sukul P, Spiteller M. Sulfonamides in the environment as veterinary drugs. Rev Environ Contam Toxicol, 2006, 187: 67–101
Burkhardt M, Stamm C, Waul C, et al. Surface runoff and transport of sulfonamide antibiotics and tracers on manured grassland. J Environ Qual, 2005, 34: 1363–1371
Zilles J, Shimada T, Jindal A, et al. Presence of macrolide-lincosamide-streptogramin B and tetracycline antimicrobials in swine waste treatment processes and amended soil. Water Environ Res, 2005, 77: 57–62
Thiele-Bruhn S. Pharmacetical antibiotic compounds in soils—A review. J Plant Nutr Soil Sci, 2003, 166: 145–167
Ungemach F R, Muller-Bahrdt D, Abraham G. Guidelines for prudent use of antimicrobials and their implications on antibiotic usage in veterinary medicine. Int J Med Microbiol, 2006, 296(Suppl 2): 33–38
Department of Nature and Ecology Conservation, Ministry of Environmental Protection of the People’s Republic of China. Pollution Investigation and Control Countermeasure for National Livestock and Poultry Breeding (in Chinese). Beijing: China Environmental Science Press, 2008. 14–103
Ministry of Agriculture of the People’s Republic of China. Breeding Standard of Swine, NY/T65-2004 (in Chinese)
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Chen, Y., Zhang, H., Luo, Y. et al. Occurrence and assessment of veterinary antibiotics in swine manures: A case study in East China. Chin. Sci. Bull. 57, 606–614 (2012). https://doi.org/10.1007/s11434-011-4830-3
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DOI: https://doi.org/10.1007/s11434-011-4830-3