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Hydrolysis of Ammonia-pretreated Sugar Cane Bagasse with Cellulase, β-Glucosidase, and Hemicellulase Preparations

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Abstract

Sugar cane bagasse consists of hemicellulose (24%) and cellulose (38%), and bioconversion of both fractions to ethanol should be considered for a viable process. We have evaluated the hydrolysis of pretreated bagasse with combinations of cellulase, β-glucosidase, and hemicellulase. Ground bagasse was pretreated either by the AFEX process (2NH3: 1 biomass, 100 °C, 30 min) or with NH4OH (0.5 g NH4OH of a 28% [v/v] per gram dry biomass; 160 °C, 60 min), and composition analysis showed that the glucan and xylan fractions remained largely intact. The enzyme activities of four commercial xylanase preparations and supernatants of four laboratory-grown fungi were determined and evaluated for their ability to boost xylan hydrolysis when added to cellulase and β-glucosidase (10 filter paper units [FPU]: 20 cellobiase units [CBU]/g glucan). At 1% glucan loading, the commercial enzyme preparations (added at 10% or 50% levels of total protein in the enzyme preparations) boosted xylan and glucan hydrolysis in both pretreated bagasse samples. Xylanase addition at 10% protein level also improved hydrolysis of xylan and glucan fractions up to 10% glucan loading (28% solids loading). Significant xylanase activity in enzyme cocktails appears to be required for improving hydrolysis of both glucan and xylan fractions of ammonia pretreated sugar cane bagasse.

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Acknowledgements

We thank Benito Stradi, Giovanna DeQueiroz, and Chang-Ho Chung for the helpful discussions and preparation of the ammonium hydroxide-pretreated bagasse, Farzaneh Teymouri for the preparation of the AFEX-pretreated bagasse, Chardcie Verret and Brian White for the assistance with the analysis of the sugars, and Daan Nel at the University of Stellenbosch for the assistance with the statistical analysis.

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  1. Bernard A. Prior

    Present address: Department of Microbiology, University of Stellenbosch, Private Bag X1, Matieland, 7602, South Africa

Authors and Affiliations

  1. LSU Agricultural Center, Audubon Sugar Institute, St. Gabriel, LA, 70776, USA

    Bernard A. Prior & Donal F. Day

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  1. Bernard A. Prior
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  2. Donal F. Day
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Correspondence to Bernard A. Prior.

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Prior, B.A., Day, D.F. Hydrolysis of Ammonia-pretreated Sugar Cane Bagasse with Cellulase, β-Glucosidase, and Hemicellulase Preparations. Appl Biochem Biotechnol 146, 151–164 (2008). https://doi.org/10.1007/s12010-007-8084-0

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  • DOI: https://doi.org/10.1007/s12010-007-8084-0

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