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Bacterial Cellulose Production by Acetobacter xylinum Strains from Agricultural Waste Products

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Abstract

Bacterial cellulose is a biopolysaccharide produced from the bacteria, Acetobacter xylinum. Static batch fermentations for bacterial cellulose production were studied in coconut and pineapple juices under 30 °C in 5-l fermenters by using three Acetobacter strains: A. xylinum TISTR 998, A. xylinum TISTR 975, and A. xylinum TISTR 893. Experiments were carried out to compare bacterial cellulose yields along with growth kinetic analysis. Results showed that A. xylinum TISTR 998 produced a bacterial cellulose yield of 553.33 g/l, while A. xylinum TISTR 893 produced 453.33 g/l and A. xylinum TISTR 975 produced 243.33 g/l. In pineapple juice, the yields for A. xylinum TISTR 893, 975, and 998 were 576.66, 546.66, and 520 g/l, respectively. The strain TISTR 998 showed the highest productivity when using coconut juice. Morphological properties of cellulose pellicles, in terms of texture and color, were also measured, and the textures were not significantly different among treatments.

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References

  1. Hon, D. (1994). Cellulose, 1, 1–25.

    Article  CAS  Google Scholar 

  2. Koyama, M., Helbert, W., Imai, T., Sugiyama, J., & Henrissat, B. (1997). Proceedings of the National Academy of Sciences of the United States of America, 94, 9091–9095.

    Article  CAS  Google Scholar 

  3. Embuscado, M. E., Marks, J. S., & BeMiller, J. N. (1994). Food Hydrocolloids, 8, 407–418.

    Article  CAS  Google Scholar 

  4. Delmer, D. P., & Amor, Y. (1995). The Plant Cell, 7, 987–1000.

    Article  CAS  Google Scholar 

  5. Delmer, D. P. (1999). Annual Review of Plant Physiology and Plant Molecular Biology, 50, 245–276.

    Article  CAS  Google Scholar 

  6. Einfeldt, L., & Klemm, D. (1997). Journal of Carbohydrate Chemistry, 16, 635–646.

    Article  CAS  Google Scholar 

  7. Koyama, M., Helbert, W., Imai, T., Sugiyama, J., & Henrissat, B. (1997). Proceedings of the National Academy of Sciences of the United States of America, 94, 9091–9095.

    Article  CAS  Google Scholar 

  8. Ross, P., Mayer, R., & Benziman, M. (1991). Microbiology and Molecular Biology Reviews, 55(1), 35–58.

    CAS  Google Scholar 

  9. Hirai, A., Tsuji, M., Yamamoto, H., & Horii, F. (1998). Cellulose, 5, 201–213.

    Article  CAS  Google Scholar 

  10. Lynd, L. R., Weimer, P. J., Vanzyl, W. H., & Pretorius, I. S. (2002). Microbiology and Molecular Biology Reviews, 66, 506–577.

    Article  CAS  Google Scholar 

  11. Schramm, M., & Hestrin, S. (1954). Biochemical Journal, 56, 163–166.

    CAS  Google Scholar 

  12. Shamolina, I. (1997). Fibre Chemistry, 29(1), 3–10.

    Article  Google Scholar 

  13. Herbert, P. J., Phipps, P. J., & Strange, R. E. (1971). London: Academic, 5, 266–272.

  14. Marx-Figini, M., & Pion, B. G. (1974). Biochimica Biophysica Acta, 338, 232.

    Google Scholar 

  15. Brown, R. M., Willison, J. H. M., & Richardson, C. L. (1976). Proceedings of the National Academy of Sciences of the United States of America, 72, 4565.

    Article  Google Scholar 

  16. Seto, A., Kojima, Y., Tonouchi, N., Tsuchida, T., & Yoshinaga, F. (1997). Bioscience, Biotechnology, and Biochemistry, 61, 735–736.

    Google Scholar 

  17. Dudman, W. F. (1995). Journal of General Microbiology, 21, 327–337.

    Google Scholar 

  18. Ishikawa, A., Matsuoka, M., Tsuchida, T., & Yoshinaga, F. (1995). Bioscience, Biotechnology, and Biochemistry, 59, 2259–2262.

    Article  CAS  Google Scholar 

  19. Premjet, S., Vorasingha, A., Somsiri, A., Ohtani, Y., & Sameshima, K. (2003). Thai Journal of Biotechnology, 4(1), 30–36.

    Google Scholar 

  20. Yamanaka, S., Watanabe, K., Kitamura, N., Iguchi, M., Mitsuhashi, S., Nishi, Y., & Uryu, M. (1989). Journal of Materials Science, 24, 3141–3145.

    Article  CAS  Google Scholar 

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Acknowledgements

This work has been supported by the Faculty of Applied Science, King Mongkut’s Institute of Technology North Bangkok, Bangkok, Thailand. The author is very much grateful to Dr. Mario Ambrosino, Oregon State University, for his kind critical reading of the manuscript.

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  1. Department of Biotechnology, Faculty of Applied Science, King Mongkut’s Institute of Technology North Bangkok, Piboonsongkram Road, Bangsue, Bangkok, 10800, Thailand

    Sasithorn Kongruang

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Kongruang, S. Bacterial Cellulose Production by Acetobacter xylinum Strains from Agricultural Waste Products. Appl Biochem Biotechnol 148, 245–256 (2008). https://doi.org/10.1007/s12010-007-8119-6

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  • DOI: https://doi.org/10.1007/s12010-007-8119-6

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