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Tannase production by solid state fermentation of cashew apple bagasse

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Abstract

The ability of Aspergillus oryzae for the production of tannase by solid state fermentation was investigated using cashew apple bagasse (CAB) as substrate. The effect of initial water content was studied and maximum enzyme production was obtained when 60 mL of water was added to 100.0 g of CAB. The fungal strain was able to grow on CAB without any supplementation but a low enzyme activity was obtained, 0.576 U/g of dry substrate (gds). Optimization of process parameters such as supplementation with tannic acid, phosphorous, and different organic and inorganic nitrogen sources was studied. The addition of tannic acid affected the enzyme production and maximum tannase activity (2.40 U/gds) was obtained with 2.5% (w/w) supplementation. Supplementation with ammonium nitrate, peptone, and yeast extract exerted no influence on tannase production. Ammonium sulphate improved the enzyme production in 3.75-fold compared with control. Based on the experimental results, CAB is a promising substrate for solid state fermentation, enabling A. oryzae growth and the production of tannase, with a maximum activity of 3.42 U/gds and enzyme productivity of 128.5×10−3 U · g −1ds · h−1.

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Authors and Affiliations

  1. Departamento de Engenharia Química, Campus do Pici, Universidade Federal do Ceará, Bloco 709, 60455-760, Fortaleza CE, Brazil

    Tigressa H. S. Rodrigues, Maria Alcilene A. Dantas & Luciana R. B. Gonçalves

  2. Embrapa Agroindústria Tropical, Rua Dra Sara Mesquita, 2270, Planalto do Pici, CEP 60511-110, Fortaleza CE, Brazil

    Gustavo A. S. Pinto

Authors
  1. Tigressa H. S. Rodrigues
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  2. Maria Alcilene A. Dantas
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  3. Gustavo A. S. Pinto
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  4. Luciana R. B. Gonçalves
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Correspondence to Luciana R. B. Gonçalves.

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Rodrigues, T.H.S., Dantas, M.A.A., Pinto, G.A.S. et al. Tannase production by solid state fermentation of cashew apple bagasse. Appl Biochem Biotechnol 137, 675–688 (2007). https://doi.org/10.1007/s12010-007-9088-5

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