Abstract
Sugar concentration from sugarcane juice and yeast autolysate increased lactic acid production more than the other agro-industrial substrates tested. The concentrations of these two components were further optimized using the Plackett–Burman design and response surface method. A second-order polynomial regression model estimated that a maximal lactic acid production of 66.11 g/L would be obtained when the optimal values of sugar and yeast autolysate were 116.9 and 44.25 g/L, respectively. To validate the optimization of the medium composition, studies were carried out using the optimized conditions to confirm the result of the response surface analysis. After 48 h, lactic acid production using the shake-flask method was at 60.2 g/L.
Similar content being viewed by others
References
Ohara, H. (2003). Applied Microbiology and Biotechnology, 62, 474–477.
Di Lorenzo, M. L. (2005). European Polymer Journal, 41, 569–575.
Ryu, H. W., Yun, J. S., Wee, Y. J. (2003), In A. Pandey (ed.), Concise encyclopedia of bioresource technology, lactic acid (p. 635). New York: The Haworth Press.
Lipinsky, E. S., & Sinclair, L. G. (1986). Chemical Engineering Progress, 82, 26–32.
Hofvendahl, K., & Hahn-Hägerdal, B. (1997). Enzyme and Microbial Technology, 20, 301–307.
Ikada, Y., Jamshidi, K., Tsuji, H., & Hyon, S. H. (1987). Macromolecules, 20, 904–906.
Tsuji, H., Hyon, S. H., & Ikada, Y. (1991). Macromolecules, 24, 5651–5656.
Fitzpatrick, J. J., & Keeffe, U. O. (2001). Process Biochemistry, 37, 183–186.
Tejayadi, S., & Cheryan, M. (1995). Applied Microbiology and Biotechnology, 43, 242–248.
Calabia, B. P., & Tokiwa, Y. (2007). Biotechnological Letters, 29, 1329–1332.
Dumbrepatil, A., Adsul, M., Chaudhari, S., Khire, J., & Gokhale, D. (2008). Applied and Environmental Microbiology, 74, 333–335.
Coelho, L. F., De Lima, C. J. B., Bernardo, M. P., Alvarez, G. M., & Contiero, J. (2010). Journal of the Science of Food and Agriculture, 90, 1944–1950.
Buyukkileci, A. O., & Harsa, S. (2004). Journal of Chemical Technology and Biotechnology, 79, 1036–1040.
Yu, L., Lei, T., Ren, X., Pei, X., & Feng, X. (2008). Biochemical Engineering Journal, 39, 496–502.
De Lima, C. J. B., Coelho, L. F., Blanco, K. C., & Contiero, J. (2009). African Journal of Biotechnology, 8, 5842–5846.
Miller, G. L. (1959). Analytical Chemistry, 31, 426–428.
Timbuntam, W., Sriroth, K., & Tokiwa, Y. (2006). Biotechnological Letters, 28, 811–814.
Vahvaselka, M. I., Linko, P. (1987). In O. M. Neijssel, R. R. Van der Meer and K. Ch A. M. Luyben (Eds.), Proceedings of the 4th European congress on biotechnology, n. 3, vol. 123: Lactic acid fermentation in milk ultrafiltrate by Lactobacillus helveticus (pp. 317–320). Amsterdam: Elsevier Science BV.
Cox, G. C., & Macbean, R. D. (1977). Australian Journal of Dairy Technology, 32, 19–22.
Hurok, O. H., Wee, W. J., Yun, J. S., Han, S. H., Jung, S., & Ryu, H. W. (2005). Bioresource Technology, 96, 1492–1498.
Naveena, B. J., Altaf, M. D., Bhadriah, K., & Reddy, G. (2005). Bioresource Technology, 96, 485–490.
Honorato, T. L., Rabelo, M. C., Pinto, G. A. S., & Rodrigues, S. (2007). Ciencia y Tecnologia Alimentaria, 27, 254–258.
Wood, B. J. B., & Holzapfel, W. H. (1995). The genera of lactic acid bacteria (1st ed.). Glasgow: Blackie Academic and Professional.
Pritchard, G., & Coolbear, T. (1993). FEMS Microbiology, 12, 179–206.
Sule, B., Elibol, M., & Ozer, D. (2004). Biochemical Engineering Journal, 21, 33–37.
Acknowledgments
The authors are grateful to Tavolaro Dairy and Corn Products for kindly supplying the whey and corn steep liquor, respectively, the Sta. Lucia plant for supplying sugarcane molasses and sugarcane juice, the Brazilian fostering agency São Paulo Research Foundation—FAPESP, and the National Council for Scientific and Technological Development (CNPq) for the fellowships and financial support.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Coelho, L.F., de Lima, C.J.B., Bernardo, M.P. et al. d(−)-Lactic Acid Production by Leuconostoc mesenteroides B512 Using Different Carbon and Nitrogen Sources. Appl Biochem Biotechnol 164, 1160–1171 (2011). https://doi.org/10.1007/s12010-011-9202-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12010-011-9202-6