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C–S Targeted Biodegradation of Dibenzothiophene by Stenotrophomonas sp. NISOC-04

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Abstract

Crude oil-contaminated soil samples were gathered across Khuzestan oilfields (National Iranian South Oil Company, NISOC) consequently experienced a screening procedure for isolating C–S targeted dibenzothiophene-biodegrading microorganisms with previously optimized techniques. Among the isolates, a bacterial strain was selected due to its capability of biodegrading dibenzothiophene in a C–S targeted manner in aqueous phases and medium mostly consisting of separately biphasic water–gasoline. The 16S rDNA of the isolate was amplified using eubacterial-specific primers and then sequenced. Based on sequence data analysis, the microorganism, designated NISOC-04, clustered most closely with the members of the genus Stenotrophomonas. Gas chromatography indicated that Stenotrophomonas sp. NISOC-04 utilizes 82% of starting 0.8 mM dibenzothiophene within a 48-h-long exponential growth phase. Growth curve analysis revealed the inability of Stenotrophomonas sp. NISOC-04 to utilize dibenzothiophene (DBT) as the exclusive carbon or carbon/sulfur source. Gibbs’ assay showed no 2-hydroxy biphenyl accumulation, but HPLC confirmed the presence of 2-hydroxy biphenyl as the final product of DBT desulfurization. Under sulfur starvation, Stenotrophomonas sp. NISOC-04 produced a huge biomass with untraceable sulfur and utilized atmospheric insignificant sulfur levels.

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Acknowledgment

This work was supported by the Research vice Deputy, Shahid Chamran University of Ahvaz, and National Iranian South Oil Company (NISOC; R&D Department, the Department of chemicals, and the laboratorial complex). We warmly appreciate efforts made by Seyed Abbas Dibaj and Abdollah Ahmadiseresht, the expert staff of the Gas Chromatography (GC) Laboratory of NISOC. We are so grateful to Man Bock Gu and Ashok Mulchandani for managing the review process and Jamaledin Adel and the reviewers for their help in improving the content of this work.

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Authors and Affiliations

  1. Biotechnology & Biology Research Center, Shahid Chamran University, Ahvaz, Iran

    Moslem Papizadeh & Mohammad Roayaei Ardakani

  2. Department of Biology, Faculty of Sciences, Shahid Chamran University, Ahvaz, Iran

    Mohammad Roayaei Ardakani & Hossein Motamedi

  3. Department of Biology, Faculty of Sciences, Shahed University, Tehran, Iran

    Iraj Rasouli & Mohammad Zarei

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  1. Moslem Papizadeh
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  2. Mohammad Roayaei Ardakani
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  3. Hossein Motamedi
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  4. Iraj Rasouli
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Correspondence to Moslem Papizadeh.

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Papizadeh, M., Ardakani, M.R., Motamedi, H. et al. C–S Targeted Biodegradation of Dibenzothiophene by Stenotrophomonas sp. NISOC-04. Appl Biochem Biotechnol 165, 938–948 (2011). https://doi.org/10.1007/s12010-011-9310-3

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  • DOI: https://doi.org/10.1007/s12010-011-9310-3

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