Abstract
An effective alkali pretreatment which affects the structural properties of cellulose (corn cob) has been studied. The pretreatment of corn cob was carried out with different combinations of alkali at varying temperatures. The most effective pretreatment of corn cob was achieved with 1 % alkali at 50 °C in 4 h. The crystallinity index (CrI) and specific surface area (SSA) of untreated corn cob was 39 % and 0.52 m2/g wherein after alkali pretreatment CrI decreased to 15 % and SSA increased to 3.32 m2/g. The fungal organism was identified as Penicillium pinophilum on the basis of ITS sequence. At 5 % substrate concentration using a complete cellulase from Penicillium pinophilum the hydrolysis of untreated corn cob with 5, 10 and 20 FPU/g enzyme loadings were 11 %, 13 % and 16 %, whereas after alkali treatment the hydrolysis increased to 78 %, 90 % and 100 %, respectively. Further hydrolytic potential of commercial cellulases viz. Accellerase™ 1,000, Palkofeel-30 and Palkocel-40 were investigated under similar conditions.
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Farrell, A. E., Plevin, R. J., Turner, B. T., Jones, A. D., O’Hare, M., & Kammen, D. M. (2006). Science, 311, 506–508.
Klemm, D., Schmauder, H. P., & Heinze, T. (2002). Cellulose. In E. J. Vandamme, S. De Baets, & A. Steinbüchel (Eds.), Biopolymers Vol. 6 (pp. 275–319). Weinheim: Wiley-VCH.
Bhat, M. K., & Bhat, S. (1997). Biotechnology Advances, 15, 583–620.
Kim, J., & Yun, S. (2006). Macromolecules, 39, 4202–4206.
Rajaram, S., & Varma, A. (1990). Applied Microbiology and Biotechnology, 34, 141–144.
Perlack, R. D., Wright, L. L., Turhollow, A. F., Graham, R. L., Stokes, B. J., & Erbach, D. C. (2005). Biomass as feed stock for bioenergy and bioproducts industry: The technical feasibility of billion ton annual supply. Oak Ridge: US Department of Energy.
Graham, R. L., Nelson, R., Sheehan, J., Perlack, R. D., & Wright, L. L. (2007). Agronomy Journal, 99, 1–11.
Barl, B., Biliaderis, G. C., Murray, D. E., & Macgregor, W. A. (1991). Journal of the Science of Food and Agriculture, 56, 195–214.
Chen, M., Xia, L., & Xue, P. (2007). International Biodeterioration & Biodegradation, 59, 5–89.
Miura, S., Arimura, T., Itoda, N., Dwiarti, L., Feng, J. B., Bin, C. H., et al. (2004). Journal of Bioscience and Bioengineering, 97, 153–157.
Karr, W. E., Gutierrez, C. V., & Kinshita, C. M. (1998). Biomass and Bioenergy, 14, 277–287.
Lau, W. M., & Dale, E. B. (2009). Proceedings of the National Academy of Sciences, 106, 1368–1373.
Shi, J., Sharma-Shivappa, R. R., Chinn, M., & Howell, N. (2009). Biomass and Bioenergy, 33, 88–96.
Kim, S., & Holtzapple, M. T. (2005). Bioresource Technology, 96, 1994–2006.
Mosier, N., Wyman, C. E., Dale, B. E., Elander, R. T., Lee, Y. Y., Holtzapple, M., et al. (2005). Bioresource Technology, 96, 673–686.
Wang, Z., Keshwani, D. R., Redding, A. P., & Cheng, J. J. (2010). Bioresource Technology, 101, 3583–3585.
Singh, R., Varma, A. J., Laxman, R. S., & Rao, M. (2009). Bioresource Technology, 100, 6679–6681.
Sluiter, A., Hames, B., Ruiz, R., Scarlata, C., Sluiter, J., Templeton, D., et al. (2008). www.nrel.gov/biomass/pdfs/42618.pdf
Lodhi, M. A., Ye, G. N., Weeden, N. F., & Reisch, B. I. (1994). Plant Molecular Biology Reporter, 12, 6–13.
White, T. J., Bruns, T., Lee, S., & Taylor, J. W. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In M. A. Innis, D. H. Gelfand, J. J. Sninsky, & T. J. White (Eds.), PCR protocols: a guide to methods and applications (pp. 315–322). New York: Academic Press.
Felsenstein, J. (1985). Evolution, 39, 783–791.
Tamura, K., Dudley, J., Nei, M., & Kumar, S. (2007). Molecular Biology and Evolution, 24, 1596–1599.
Ghose, T. K. (1987). Pure and Applied Chemistry, 59, 257–268.
Ghose, T. K., & Bisaria, V. S. (1987). Pure and Applied Chemistry, 59, 1739–1752.
Klug, H. P., & Alexender, L. E. (1954). X-ray diffraction procedures for polycrystalline and amorphous materials (2nd ed.). New York: Wily.
Segal, L., Creely, J. J., Martin, A. E., Jr., & Conrad, C. M. (1962). Textile Research Journal, 29, 786–794.
Carberry, J. J. (1976). Chemical and catalytic reaction engineering. New York: McGraw-Hill.
Fan, L. T., Gharpuray, M. M., & Lee, Y. H. (1987). Cellulose hydrolysis biotechnology monographs (p. 57). Berlin: Springer.
Tarkov, H., & Feist, W. C. (1969). Advances in Chemistry Series, 95, 197–218.
Gharpuray, M. M., Lee, Y. H., & Fan, L. T. (1983). Biotechnology and Bioengineering, 25, 157–172.
Thompson, D. N., Chen, H. C., & Grethlein, H. E. (1992). Bioresource Technology, 39, 155–163.
Park, S., Baker, O. J., Himmel, E. M., Parilla, A. P., & Johnson, A. P. (2010). Biotechnology for Biofuels, 3, 1–10.
Kaar, W. E., & Holtzapple, M. (1998). Biotechnology and Bioengineering, 59, 419–427.
Hemmatinejad, N., Vahabzadeh, F., & Koredestani, S. S. (2002). Iranian Polymer Journal, 11, 333–338.
Acknowledgements
M.R. acknowledges the financial support from CSIR Emeritus scheme. M.R. also acknowledges Vishnu and Dipali for their assistance. The authors are thankful to Drs. Yogesh Souche and Shiv Shankar for their valuable help in molecular identification of the isolate.
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Sahare, P., Singh, R., Laxman, R.S. et al. Effect of Alkali Pretreatment on the Structural Properties and Enzymatic Hydrolysis of Corn Cob. Appl Biochem Biotechnol 168, 1806–1819 (2012). https://doi.org/10.1007/s12010-012-9898-y
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DOI: https://doi.org/10.1007/s12010-012-9898-y