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Phytohormone Supplementation Significantly Increases Growth of Chlamydomonas reinhardtii Cultivated for Biodiesel Production

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Abstract

Cultivation is the most expensive step in the production of biodiesel from microalgae, and substantial research has been devoted to developing more cost-effective cultivation methods. Plant hormones (phytohormones) are chemical messengers that regulate various aspects of growth and development and are typically active at very low concentrations. In this study, we investigated the effect of different phytohormones on microalgal growth and biodiesel production in Chlamydomonas reinhardtii and their potential to lower the overall cost of commercial biofuel production. The results indicated that all five of the tested phytohormones (indole-3-acetic acid, gibberellic acid, kinetin, 1-triacontanol, and abscisic acid) promoted microalgal growth. In particular, hormone treatment increased biomass production by 54 to 69 % relative to the control growth medium (Tris–acetate–phosphate, TAP). Phytohormone treatments also affected microalgal cell morphology but had no effect on the yields of fatty acid methyl esters (FAMEs) as a percent of biomass. We also tested the effect of these phytohormones on microalgal growth in nitrogen-limited media by supplementation in the early stationary phase. Maximum cell densities after addition of phytohormones were higher than in TAP medium, even when the nitrogen source was reduced to 40 % of that in TAP medium. Taken together, our results indicate that phytohormones significantly increased microalgal growth, particularly in nitrogen-limited media, and have potential for use in the development of efficient microalgal cultivation for biofuel production.

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References

  1. Brune, D., Lundquist, T., & Benemann, J. (2009). Microalgal biomass for greenhouse gas reductions: potential for replacement of fossil fuels and animal feeds. Journal of Environmental Engineering, 135, 1136–44.

    Article  CAS  Google Scholar 

  2. Demirbas, A. (2009). Progress and recent trends in biodiesel fuels. Energy Conversion and Management, 50, 14–34.

    Article  CAS  Google Scholar 

  3. Gouveia, L., & Oliveira, A. (2009). Microalgae as a raw material for biofuels production. Journal of Industrial Microbiology & Biotechnology, 36, 269–74.

    Article  CAS  Google Scholar 

  4. Harun, R., Davidson, M., Doyle, M., Gopiraj, R., Danquah, M., & Forde, G. (2011). Technoeconomic analysis of an integrated microalgae photobioreactor, biodiesel and biogas production facility. Biomass and Bioenergy, 35, 741–7.

    Article  CAS  Google Scholar 

  5. Hu, B., Min, M., Zhou, W. G., Li, Y. C., Mohr, M., et al. (2012). Influence of exogenous CO2 on biomass and lipid accumulation of microalgae Auxenochlorella protothecoides cultivated in concentrated municipal wastewater. Applied Biochemistry and Biotechnology, 166, 1661–73.

    Article  CAS  Google Scholar 

  6. Liu, J. H., Yuan, C., Hu, G. R., & Li, F. L. (2012). Effects of light intensity on the growth and lipid accumulation of Microalga Scenedesmus sp 11–1 under nitrogen limitation. Applied Biochemistry and Biotechnology, 166, 2127–37.

    Article  CAS  Google Scholar 

  7. Sheng, J., Kim, H. W., Badalamenti, J. P., Zhou, C., Sridharakrishnan, S., et al. (2011). Effects of temperature shifts on growth rate and lipid characteristics of Synechocystis sp. PCC6803 in a bench-top photobioreactor. Bioresource Technology, 102, 11218–25.

    Article  CAS  Google Scholar 

  8. Zheng, Y., Chi, Z., Lucker, B., & Chen, S. (2012). Two-stage heterotrophic and phototrophic culture strategy for algal biomass and lipid production. Bioresource Technology, 103, 484–8.

    Article  CAS  Google Scholar 

  9. Pruvost, J., Cornet, J. F., & Legrand, J. (2008). Hydrodynamics influence on light conversion in photobioreactors: an energetically consistent analysis. Chemical Engineering Science, 63, 3679–94.

    Article  CAS  Google Scholar 

  10. Carvalho, A., Silva, S., Baptista, J., & Malcata, F. (2011). Light requirements in microalgal photobioreactors: an overview of biophotonic aspects. Applied Microbiology and Biotechnology, 89, 1275–88.

    Article  CAS  Google Scholar 

  11. Chen, C.-Y., Yeh, K.-L., Aisyah, R., Lee, D.-J., & Chang, J.-S. (2011). Cultivation, photobioreactor design and harvesting of microalgae for biodiesel production: a critical review. Bioresource Technology, 102, 71–81.

    Article  CAS  Google Scholar 

  12. Rubio, V., Bustos, R., Irigoyen, M. L., Cardona-Lopez, X., Rojas-Triana, M., & Paz-Ares, J. (2009). Plant hormones and nutrient signaling. Plant Molecular Biology, 69, 361–73.

    Article  CAS  Google Scholar 

  13. Chatterjee, S., Chatterjee, S., Chatterjee, B. P., & Guha, A. K. (2008). Enhancement of growth and chitosan production by Rhizopus oryzae in whey medium by plant growth hormones. International Journal of Biological Macromolecules, 42, 120–6.

    Article  CAS  Google Scholar 

  14. Vance, B. D. (1987). Phytohormone effects on cell division in Chlorella pyrenoidosa chick (TX-7-11-05) (chlorellaceae). Journal of Plant Growth Regulation, 5, 169–73.

    Article  CAS  Google Scholar 

  15. Czerpak, R., Bajguz, A., Gromek, M., Kozłowska, G., & Nowak, I. (2002). Activity of salicylic acid on the growth and biochemism of Chlorella vulgaris Beijerinck. Acta Physiologiae Plantarum, 24, 45–52.

    Article  CAS  Google Scholar 

  16. Tate, J., Gutierrez-Wing, M. T., Rusch, K., & Benton, M. (2013). The effects of plant growth substances and mixed cultures on growth and metabolite production of green algae Chlorella sp.: a review. Journal of Plant Growth Regulation, 32, 417–28.

    Article  CAS  Google Scholar 

  17. Piotrowska, A., Czerpak, R., Pietryczuk, A., Olesiewicz, A., & Wędołowska, M. (2008). The effect of indomethacin on the growth and metabolism of green alga Chlorella vulgaris Beijerinck. Plant Growth Regulation, 55, 125–36.

    Article  CAS  Google Scholar 

  18. Hunt, R. W., Chinnasamy, S., Bhatnagar, A., & Das, K. C. (2010). Effect of biochemical stimulants on biomass productivity and metabolite content of the microalga, Chlorella sorokiniana. Applied Biochemistry and Biotechnology, 162, 2400–14.

    Article  CAS  Google Scholar 

  19. Yoshida, K., Igarashi, E., Mukai, M., Hirata, K., & Miyamoto, K. (2003). Induction of tolerance to oxidative stress in the green alga, Chlamydomonas reinhardtii, by abscisic acid. Plant, Cell & Environment, 26, 451–7.

    Article  CAS  Google Scholar 

  20. Ludwig-Müller, J. (2011). Auxin conjugates: their role for plant development and in the evolution of land plants. Journal of Experimental Botany, 62, 1757–73.

    Article  Google Scholar 

  21. Tarakhovskaya, E. R., Maslov, Y. I., & Shishova, M. F. (2007). Phytohormones in algae. Russian Journal of Plant Physiology, 54, 163–70.

    Article  CAS  Google Scholar 

  22. Kiseleva, A. A., Tarachovskaya, E. R., & Shishova, M. F. (2012). Biosynthesis of phytohormones in algae. Russian Journal of Plant Physiology, 59, 595–610.

    Article  CAS  Google Scholar 

  23. Gorman, D. S., & Levine, R. P. (1965). Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardtii. Proceedings of the National Academy of Sciences, 54, 1665–9.

    Article  CAS  Google Scholar 

  24. Ritchie, R. J. (2006). Consistent sets of spectrophotometric chlorophyll equations for acetone, methanol and ethanol solvents. Photosynthesis Research, 89, 27–41.

    Article  CAS  Google Scholar 

  25. Brányiková, I., Maršálková, B., Doucha, J., Brányik, T., Bišová, K., et al. (2011). Microalgae—novel highly efficient starch producers. Biotechnology and Bioengineering, 108, 766–76.

    Article  Google Scholar 

  26. Folch, J., Lees, M., & Stanley, G. H. S. (1957). A simple method for the isolation and purification of total lipides from animal tissues. Journal of Biological Chemistry, 226, 497.

    CAS  Google Scholar 

  27. De-Bashan, L. E., Antoun, H., & Bashan, Y. (2008). Involvement of indole-3-acetic acid produced by the growth-promoting bacterium Azospirillum spp. in promoting growth of Chlorella vulgaris1. Journal of Phycology, 44, 938–47.

    Article  CAS  Google Scholar 

  28. Gonzalez, L. E., & Bashan, Y. (2000). Increased growth of the microalga Chlorella vulgaris when coimmobilized and cocultured in alginate beads with the plant-growth-promoting bacterium Azospirillum brasilense. Applied and Environmental Microbiology, 66, 1527–31.

    Article  CAS  Google Scholar 

  29. Shibaoka, H. (1994). Plant hormone-induced changes in the orientation of cortical microtubules: alterations in the cross-linking between microtubules and the plasma membrane. Annual Review of Plant Physiology and Plant Molecular Biology, 45, 527–44.

    Article  CAS  Google Scholar 

  30. Matsumura, K., Yagi, T., Hattori, A., Soloviev, M., & Yasuda, K. (2010). Using single cell cultivation system for on-chip monitoring of the interdivision timer in Chlamydomonas reinhardtii cell cycle. Journal of Nanobiotechnology, 8, 1–13.

    Article  Google Scholar 

  31. Lee, K., & Lee, C.-G. (2001). Effect of light/dark cycles on wastewater treatments by microalgae. Biotechnology and Bioprocess Engineering, 6, 194–9.

    Article  CAS  Google Scholar 

  32. Amaro, H. M., Guedes, A. C., & Malcata, F. X. (2011). Advances and perspectives in using microalgae to produce biodiesel. Applied Energy, 88, 3402–10.

    Article  CAS  Google Scholar 

  33. Illman, A. M., Scragg, A. H., & Shales, S. W. (2000). Increase in Chlorella strains calorific values when grown in low nitrogen medium. Enzyme and Microbial Technology, 27, 631–5.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon, 305-701, Republic of Korea

    Won-Kun Park, Gursong Yoo, Myounghoon Moon, Chul Woong Kim & Ji-Won Yang

  2. LED Agri-bio Fusion Technology Research Center, Chonbuk National University, 79 Gobong-ro, Iksan-si, Jeollabuk-do, 570-752, Republic of Korea

    Yoon-E Choi

  3. Advanced Biomass R&D Center, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon, 305-701, Republic of Korea

    Ji-Won Yang

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  1. Won-Kun Park
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  2. Gursong Yoo
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  3. Myounghoon Moon
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Correspondence to Yoon-E Choi or Ji-Won Yang.

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Park, WK., Yoo, G., Moon, M. et al. Phytohormone Supplementation Significantly Increases Growth of Chlamydomonas reinhardtii Cultivated for Biodiesel Production. Appl Biochem Biotechnol 171, 1128–1142 (2013). https://doi.org/10.1007/s12010-013-0386-9

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  • DOI: https://doi.org/10.1007/s12010-013-0386-9

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