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LC-MS/MS Based Identification of Piperine Production by Endophytic Mycosphaerella sp. PF13 from Piper nigrum

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Abstract

Piper nigrum is very remarkable for its medicinal properties due to the presence of metabolites like piperine. Emerging understanding on the biosynthetic potential of endophytic fungi suggests the possibility to have piperine producing fungi in P. nigrum. In the current study, endophytic fungi isolated from P. nigrum were screened for the presence of piperine by liquid chromatography-tandem mass spectrometry (LC-MS/MS). This resulted in the identification of a Mycosphaerella sp. with the ability to produce piperine extracellularly. The biosynthesis of piperine (C17H19NO3) by the endophytic fungal isolate was confirmed by the presence of m/z 286.1 (M + H+) in the LC-MS/MS analysis using positive mode ionization. This was further supported by the presence of specific fragment ions with masses 135, 143, 171 and 201 formed due to the fragmentation of piperine present in the fungal extract.

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Acknowledgments

This study was supported by the Department of Biotechnology (DBT), Government of India, under DBT-RGYI and DBT-MSUB support scheme and the Department of Science and Technology, Government of India, under DST-PURSE programme.

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Authors and Affiliations

  1. School of Biosciences, Mahatma Gandhi University, PD Hills (PO), Kottayam, Kerala, 686560, India

    S. Chithra, B. Jasim, C. Anisha, Jyothis Mathew & E. K. Radhakrishnan

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  1. S. Chithra
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  2. B. Jasim
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  3. C. Anisha
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  4. Jyothis Mathew
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  5. E. K. Radhakrishnan
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Correspondence to E. K. Radhakrishnan.

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Chithra, S., Jasim, B., Anisha, C. et al. LC-MS/MS Based Identification of Piperine Production by Endophytic Mycosphaerella sp. PF13 from Piper nigrum . Appl Biochem Biotechnol 173, 30–35 (2014). https://doi.org/10.1007/s12010-014-0832-3

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  • DOI: https://doi.org/10.1007/s12010-014-0832-3

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