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Production of Cellulolytic Enzymes and Application of Crude Enzymatic Extract for Saccharification of Lignocellulosic Biomass

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Abstract

In this study, the optimal conditions for production of cellulolytic enzymes by Trichoderma reesei NRRL-6156 using the solid-state fermentation were assessed in conical flasks and validated in a packed-bed bioreactor. Afterwards, the crude enzymatic extract obtained in the optimized condition was used for hydrolysis of sugarcane bagasse in water and ultrasound baths. The enzyme activities determined in this work were filter paper, exocellulase, endocellulase, and xylanase. The optimized condition for production was moisture content 68.6 wt% and soybean bran concentration 0.9 wt%. The crude enzymatic extract was applied for hydrolysis of sugarcane bagasse, being obtained 224.0 and 229 g kg−1 at temperature of 43.4 °C and concentration of enzymatic extract of 18.6 % in water and ultrasound baths, respectively. The yields obtained are comparable to commercial enzymes.

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Acknowledgments

The authors thank SCIT-RS for the financial support of this work as well as CAPES and CNPq for scholarships.

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Authors and Affiliations

  1. Department of Chemical Engineering, Federal University of Santa Maria, Av. Roraima, 1000, Santa Maria, 97105-900, Brazil

    Juliana Machado Gasparotto, Leandro Bernardi Werle, Edson L. Foletto, Raquel C. Kuhn, Sergio Luiz Jahn & Marcio A. Mazutti

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  1. Juliana Machado Gasparotto
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  2. Leandro Bernardi Werle
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  3. Edson L. Foletto
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  4. Raquel C. Kuhn
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  5. Sergio Luiz Jahn
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  6. Marcio A. Mazutti
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Correspondence to Marcio A. Mazutti.

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Gasparotto, J.M., Werle, L.B., Foletto, E.L. et al. Production of Cellulolytic Enzymes and Application of Crude Enzymatic Extract for Saccharification of Lignocellulosic Biomass. Appl Biochem Biotechnol 175, 560–572 (2015). https://doi.org/10.1007/s12010-014-1297-0

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  • DOI: https://doi.org/10.1007/s12010-014-1297-0

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