Abstract
In this study, corncob acid hydrolysate was used as a substrate for bacterial cellulose (BC) production by Gluconacetobacter xylinus. After 2 weeks’ static fermentation, a BC yield of 4 g/L could be obtained. Both effects of medium composition and fermentation condition on the BC production were evaluated. Most extra substrates (carbon and nitrogen sources) except mannitol, butyric acid, and levulinic acid showed no effect on the improvement of BC yield. Fermentation condition including fermentation mode, inoculation concentration, and initial pH showed certain influence on the BC yield and thus should be well controlled. The analysis by field emission scanning electron microscope (FE-SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) showed that the BC sample had obvious nano-network structure, clear functional groups that were found in cellulose, and relatively high crystallinity and crystallinity index value. Moreover, the BC sample had great water-holding capacity. Overall, corncob acid hydrolysate could be one promising substrate for BC production.
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Acknowledgments
The authors acknowledge the financial support of the Science and Technology Project of Guangdong Province (2013B010403020), National Natural Science Foundation of China (51303181), the Support Plan Project of National Science and Technology (2012BAD32B07), the Project of Jiangsu Province Science and Technology (BE2013083), and Foundation of Director of Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences (y407pb1001, y107rf1001).
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Huang, C., Yang, XY., Xiong, L. et al. Utilization of Corncob Acid Hydrolysate for Bacterial Cellulose Production by Gluconacetobacter xylinus . Appl Biochem Biotechnol 175, 1678–1688 (2015). https://doi.org/10.1007/s12010-014-1407-z
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DOI: https://doi.org/10.1007/s12010-014-1407-z