Abstract
Oxalic acid was evaluated as an alternative reagent to mineral inorganic acid in pretreatment of corncob to achieve high xylose yield in addition to highly digestible solid residue. A quadratic polynomial model of xylose formation was developed for optimization of pretreatment process by the response surface methodology based on the impact factors of pretreatment temperature, reaction time, acid concentration, and solid-to-liquid ratio. The highest xylose yield was 94.3 % that was obtained under the pretreatment condition of 140 °C for 40 min with 0.5 wt% oxalic acid at a solid loading of 7.5 %. Under these conditions, the xylose yield results of verification experiments were very close to the model prediction, which indicated that the model was applicable. The solid residue generated under this condition also demonstrated a satisfactory enzymatic digestibility and fermentability.
Similar content being viewed by others
References
Lynd, L. R., van Zyl, W. H., McBride, J. E., & Laser, M. (2005). Consolidated bioprocessing of cellulosic biomass: an update. Current Opinion in Biotechnology, 16, 577–583.
Moe, S. T., Janga, K. K., Hertzberg, T., Hägg, M.-B., Øyaas, K., & Dyrset, N. (2012). Saccharification of lignocellulosic biomass for biofuel and biorefinery applications—a renaissance for the concentrated acid hydrolysis? Energy Procedia, 20, 50–58.
Berndes, G., Hoogwijk, M., & van den Broek, R. (2003). The contribution of biomass in the future global energy supply: a review of 17 studies. Biomass and Bioenerg, 25, 1–28.
Kabel, M. A., Bos, G., Zeevalking, J., Voragen, A. G., & Schols, H. A. (2007). Effect of pretreatment severity on xylan solubility and enzymatic breakdown of the remaining cellulose from wheat straw. Bioresource Technology, 98, 2034–2042.
Kahar, P., Taku, K., & Tanaka, S. (2010). Enzymatic digestion of corncobs pretreated with low strength of sulfuric acid for bioethanol production. Journal of Bioscience and Bioengineering, 110, 453–458.
He, Y.-C., Liu, F., Gong, L., Lu, T., Ding, Y., Zhang, D.-P., Qing, Q., & Zhang, Y. (2015). Improving enzymatic hydrolysis of corn stover pretreated by ethylene glycol-perchloric acid-water mixture. Applied Biochemistry and Biotechnology, 175, 1306–1317.
Himmel, M. E., Ding, S. Y., Johnson, D. K., Adney, W. S., Nimlos, M. R., & Brady, J. W. (2007). Biomass recalcitrance: engineering plants and enzymes for biofuels production. Science, 315, 804–807.
Zhou X, Xu J, Wang Z, Cheng JJ, Li R, & Qu R. (2012)._Dilute sulfuric acid pretreatment of transgenic switchgrass for sugar production. Bioresource technology, 104, 823–827.
Saha, B. C. (2003). Hemicellulose bioconversion. Journal of Industrial Microbiology & Biotechnology, 30, 279–291.
Yang, B., & Wyman, C. E. (2004). Effect of xylan and lignin removal by batch and flowthrough pretreatment on the enzymatic digestibility of corn stover cellulose. Biotechnology and Bioengineering, 86, 88–95.
Zhang, T., Kumar, R., Tsai, Y.-D., Elander, R. T., & Wyman, C. E. (2015). Xylose yields and relationship to combined severity for dilute acid post-hydrolysis of xylooligomers from hydrothermal pretreatment of corn stover. Green Chemistry, 17, 394–403.
Latif, F., & Rajoka, M. I. (2001). Production of ethanol and xylitol from corn cobs by yeasts. Bioresource Technology, 77, 57–63.
Avci, A., Saha, B. C., Dien, B. S., Kennedy, G. J., & Cotta, M. A. (2013). Response surface optimization of corn stover pretreatment using dilute phosphoric acid for enzymatic hydrolysis and ethanol production. Bioresource Technology, 130, 603–612.
Dong, Q., Zhang, S., Zhang, L., Ding, K., & Xiong, Y. (2015). Effects of four types of dilute acid washing on moso bamboo pyrolysis using Py-GC/MS. Bioresource Technology, 185, 62–69.
Toquero, C., & Bolado, S. (2014). Effect of four pretreatments on enzymatic hydrolysis and ethanol fermentation of wheat straw. Influence of inhibitors and washing. Bioresource technology, 157, 68–76.
Kootstra, A. M. J., Beeftink, H. H., Scott, E. L., & Sanders, J. P. M. (2009). Comparison of dilute mineral and organic acid pretreatment for enzymatic hydrolysis of wheat straw. Biochemical Engineering Journal, 46, 126–131.
Qin, L., Liu, Z. H., Li, B. Z., Dale, B. E., & Yuan, Y. J. (2012). Mass balance and transformation of corn stover by pretreatment with different dilute organic acids. Bioresource Technology, 112, 319–326.
Lu, Y. L., & Mosier, N. S. (2007). Biomimetic catalysis for hemicelluloses hydrolysis in corn stover. Biotechnology Progress, 23(1), 116–123.
Mosier, S. N., Ladisch, C. M., & Ladisch, M. R. (2002). Characterization of acid catalytic domains for cellulose hydrolysis and glucose degradation. Biotechnology and Bioengineering, 79, 610–618.
Scordia, D., Cosentino, S. L., Lee, J.-W., & Jeffries, T. W. (2011). Dilute oxalic acid pretreatment for biorefining giant reed (Arundo donax L.). Biomass and Bioenergy, 35, 3018–3024.
Tan, I. S., & Lee, K. T. (2014). Enzymatic hydrolysis and fermentation of seaweed solid wastes for bioethanol production: an optimization study. Energy, 78, 53–62.
Sluiter, A., Hames, B., Ruiz, R., Scarlata, C., Sluiter, J., Templeton, D., & Crocker, D. (2008). Determination of structural carbohydrates and lignin in biomass. National Renewable Energy Laboratory.
Neuriter, M., Danner, H., Thomaser, C., Saidi, B., & Braun, R. (2002). Dilute-acid hydrolysis of sugarcane bagasse at varying conditions. Applied Biochemistry and Biotechnology, 02, 50–58.
Kabel, M. A., Bos, G., Zeevalking, J., Voragen, A. G. J., & Schols, H. A. (2007). Effect of pretreatment severity on xylan solubility and enzymatic breakdown of the remaining cellulose from wheat straw. Bioresource Technology, 98, 2034–2042.
Yang, B., & Wyman, C. E. (2004). Effect of xylan and lignin removal by batch and flowthrough pretreatment on the enzymatic digestibility of corn stover cellulose. Biotechnology and Bioengineering, 86, 88–95.
Conde-Mejía, C., Jiménez-Gutiérrez, A., & El-Halwagi, M. (2012). A comparison of pretreatment methods for bioethanol production from lignocellulosic materials. Process Safety and Environmental Protection, 90, 189–202.
Bujanga, N., Rodhia, M. N. M., Musaa, M., Subaria, F., Idrisa, N., Makhtara, N. S. M., & Ku Halim, K. H. (2013). Effect of dilute sulfuric acid hydrolysis of coconut dregs on chemical and thermal properties. Procedia Engineering, 68, 372–378.
Aden, A., & Foust, T. (2009). Technoeconomic analysis of the dilute sulfuric acid and enzymatic hydrolysis process for the conversion of corn stover to ethanol. Cellulose, 16, 535–545.
Zhang, B., Wang, L., Shahbazi, A., Diallo, O., & Whitmore, A. (2011). Dilute-sulfuric acid pretreatment of cattails for cellulose conversion. Bioresource Technology, 102, 9308–9312.
Wang, W., Zhang, P., Zhang, S., Li, F., Yu, J., & Lin, J. (2013). Structure and properties of novel regenerated cellulose fibers prepared in NaOH complex solution. Carbohydrate Polymers, 98, 1031–1038.
Acknowledgments
The authors gratefully acknowledge the funding from Jiangsu Natural Science Funds through the contract number of BK20140258 and Changzhou Sci & Tech Program through the grant number of CE20145053. We also acknowledge the Laboratory of Cellulosic Biofuel, Changzhou University, for providing the facilities and equipments used in this research.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Qing, Q., Huang, M., He, Y. et al. Dilute Oxalic Acid Pretreatment for High Total Sugar Recovery in Pretreatment and Subsequent Enzymatic Hydrolysis. Appl Biochem Biotechnol 177, 1493–1507 (2015). https://doi.org/10.1007/s12010-015-1829-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12010-015-1829-2