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Sublethal Effect of Copper Toxicity Against Histopathological Changes in the Spiny Lobster, Panulirus homarus (Linnaeus, 1758)

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Abstract

The tissue damage induced by various organic pollutants in aquatic animals is well documented, but there is a dearth of information relating to the histological alterations induced by copper in the spiny lobster. In the present study, intermoult juveniles of the spiny lobster Panulirus homarus (average weight 150–200 g) were exposed to two sublethal concentrations of the copper (9.55 and 19.1 μg/l) for a period of 28 days. The muscle, hepatopancreas, midgut, gills, thoracic ganglion and heart of the lobsters were then dissected out and processed for light microscopic studies. Exposure to copper was found to result in several alterations in the histoarchitecture of the muscle, hepatopancreas, midgut, gills, thoracic ganglion and heart of P. homarus. The alterations included disruption and congestion of muscle bundle in muscle tissue; blackened haemocytes; distended lumen and F cell; necrosis of the tubules of the hepatopancreas; disarrangement of circular muscle of the midgut; accumulation of haemocytes in the haemocoelic space; swelling and fusion of lamellae; abnormal gill tips; hyperplastic, necrotic, and blackened secondary gill lamellae of the gills; damaged neurosecretory cell and sensory and motor fibre; necrotic of the thoracic ganglion; dispersedly arranged muscle bands; clumped satellite cells and nucleus of the heart. The results obtained suggest that the muscle, hepatopancreas, midgut, gills, thoracic ganglion and heart of lobsters exposed to copper were structurally altered. Such alterations could affect vital physiological functions, such as absorption, storage and secretion of the hepatopancreas, digestion of gut and respiration, osmotic and ionic regulations of the gills, which in turn could ultimately affect the survival and growth of P. homarus. Thus, all possible remedial measures should be adopted to prevent the occurrence of copper contamination in the aquatic environment.

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Authors and Affiliations

  1. PG and Research Department of Zoology, Khadir Mohideen College, Adirampattinam—614 701, Thanjavur District, Tamil Nadu, India

    A. Maharajan & P. Kumarasamy

  2. Department of Zoology, Kanchi Mamunivar Centre for Post Graduate Studies, Puducherry, India

    S. Rajalakshmi

  3. National Institute of Ocean Technology, Velacherry-Tambaram Road, Pallikaranai, Chennai, 601 302, India

    M. Vijayakumaran

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  1. A. Maharajan
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  2. S. Rajalakshmi
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  3. M. Vijayakumaran
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  4. P. Kumarasamy
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Correspondence to A. Maharajan.

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Maharajan, A., Rajalakshmi, S., Vijayakumaran, M. et al. Sublethal Effect of Copper Toxicity Against Histopathological Changes in the Spiny Lobster, Panulirus homarus (Linnaeus, 1758). Biol Trace Elem Res 145, 201–210 (2012). https://doi.org/10.1007/s12011-011-9173-z

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