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Mesoporous Silica Nanoparticles for Cancer Therapy: Energy-Dependent Cellular Uptake and Delivery of Paclitaxel to Cancer Cells

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NanoBiotechnology

Abstract

Biocompatible mesoporous silica nanoparticles, containing the fluorescence dye fluorescein isothiocyanate (FITC), provide a promising system to deliver hydrophobic anticancer drugs to cancer cells. In this study, we investigated the mechanism of uptake of fluorescent mesoporous silica nanoparticles (FMSN) by cancer cells. Incubation with FMSN at different temperatures showed that the uptake was higher at 37°C than at 4°C. Metabolic inhibitors impeded uptake of FMSN into cells. The inhibition of FMSN uptake by nocodazole treatment suggests that microtubule functions are required. We also report utilization of mesoporous silica nanoparticles to deliver a hydrophobic anticancer drug paclitaxel to PANC-1 cancer cells and to induce inhibition of proliferation. Mesoporous silica nanoparticles may provide a valuable vehicle to deliver hydrophobic anticancer drugs to human cancer cells.

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Acknowledgments

This work is supported by US NIH grants CA32737 (F.T.) and ES015498 (A.N.), NSF grant DMR0346601 (J.Z.) and a grant from Edna E. and Susan E. Riley foundation. Monty Liong and Michael Kovochich are supported by the UC Lead Campus for Nanotoxicology Training and Research, funded by UC TSR&TP.

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Authors and Affiliations

  1. Department of Microbiology, Immunology and Molecular Genetics, Jonsson Comprehensive Cancer Center, Molecular Biology Institute, University of California Los Angeles, 405 Hilgard Avenue, Los Angeles, CA, 90095, USA

    Jie Lu, Sean Sherman & Fuyuhiko Tamanoi

  2. Department of Chemistry and Biochemistry, University of California Los Angeles, 405 Hilgard Avenue, Los Angeles, CA, 90095, USA

    Monty Liong & Jeffrey I. Zink

  3. Department of Medicine, University of California Los Angeles, 405 Hilgard Avenue, Los Angeles, CA, 90095, USA

    Tian Xia, Michael Kovochich & Andre E. Nel

  4. California NanoSystems Institute, University of California Los Angeles, 405 Hilgard Avenue, Los Angeles, CA, 90095, USA

    Jie Lu, Monty Liong, Sean Sherman, Tian Xia, Michael Kovochich, Andre E. Nel, Jeffrey I. Zink & Fuyuhiko Tamanoi

  5. Department of Microbiology, Immunology and Molecular Genetics, California NanoSystems Institute, JCCC, University of California Los Angeles, 405 Hilgard Avenue, Los Angeles, CA, 90095, USA

    Fuyuhiko Tamanoi

Authors
  1. Jie Lu
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  2. Monty Liong
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  3. Sean Sherman
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  4. Tian Xia
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  5. Michael Kovochich
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  6. Andre E. Nel
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  7. Jeffrey I. Zink
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  8. Fuyuhiko Tamanoi
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Corresponding authors

Correspondence to Jeffrey I. Zink or Fuyuhiko Tamanoi.

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Lu, J., Liong, M., Sherman, S. et al. Mesoporous Silica Nanoparticles for Cancer Therapy: Energy-Dependent Cellular Uptake and Delivery of Paclitaxel to Cancer Cells. Nanobiotechnol 3, 89–95 (2007). https://doi.org/10.1007/s12030-008-9003-3

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  • DOI: https://doi.org/10.1007/s12030-008-9003-3

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