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Bioconversion of Lignocellulose into Bioethanol: Process Intensification and Mechanism Research

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Abstract

Biofuels produced from lignocellulosic biomass can significantly reduce the energy dependency on fossil fuels and the resulting effects on environment. In this respect, cellulosic ethanol as an alternative fuel has the potential to become a viable energy source in the near future. Over the past few decades, tremendous effort has been undertaken to make cellulosic ethanol cost competitive with conventional fossil fuels. The pretreatment step is always necessary to deconstruct the recalcitrant structures and to make cellulose more accessible to enzymes. A large number of pretreatment technologies involving physical, chemical, biological, and combined approaches have been developed and tested at the pilot scale. Furthermore, various strategies and methods, including multi-enzyme complex, non-catalytic additives, enzyme recycling, high solids operation, design of novel bioreactors, and strain improvement have also been implemented to improve the efficiency of subsequent enzymatic hydrolysis and fermentation. These technologies provide significant opportunities for lower total cost, thus making large-scale production of cellulosic ethanol possible. Meanwhile, many researchers have focused on the key factors that limit cellulose hydrolysis, and analyzing the reaction mechanisms of cellulase. This review describes the most recent advances on process intensification and mechanism research of pretreatment, enzymatic hydrolysis, and fermentation during the production of cellulosic ethanol.

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Abbreviations

SSF:

Simultaneous saccharification and fermentation

SSCF:

Simultaneous saccharification and co-fermentation

ARP:

Ammonia recycle percolation

SAA:

Soaking in aqueous ammonia

AFEX:

Ammonia fiber explosion

HCW:

Hot-compressed water

NMO:

N-methyl-morpholine-N-oxide

EG:

Endoglucanase

CBH:

Cellobiohydrolase

BG:

β-glucosidase

CBR:

Conventional batch reactor

MBR:

Membrane bioreactor

RBR:

Roller bottle reactors

CBM:

Cellulose-binding module

CAC:

Cellulose accessibility to cellulase

DP:

Degree of polymerization

SEC:

Size exclusion chromatography

MALLS:

Multi-angle laser light scattering

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Acknowledgments

The authors acknowledge the financial support received from Natural Science Foundation of China (20976130 and 20806057), the International Science and Technology Cooperation Program (2006DA62400), National Key Technology R&D program (2007BAD42B02), the Ministry of Education (NCET-08-0386 and 200800561004), and Tianjin R&D program (2010-BK17C004).

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  1. State Key Laboratory of Chemical Engineering, Tianjin Key Laboratory of Membrane Science and Desalination Technology, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China

    Renliang Huang, Rongxin Su, Wei Qi & Zhimin He

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Correspondence to Rongxin Su.

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Huang, R., Su, R., Qi, W. et al. Bioconversion of Lignocellulose into Bioethanol: Process Intensification and Mechanism Research. Bioenerg. Res. 4, 225–245 (2011). https://doi.org/10.1007/s12155-011-9125-7

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