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Italian ryegrass (Lolium multiflorum Lam) as a High-Potential Bio-Ethanol Resource

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Abstract

Cellulosic bioethanol production from lignocellulosic biomass has been recognized as one of the promising sustainable energy source alternatives to petroleum-based fuels, since the lignocelluloses are not directly in competition with food sources. In order to develop a lignocellulosic raw material with high energy crops for bio-ethanol production, we selected Italian ryegrass (Lolium multiflorum Lam). Bio-ethanol production from Italian ryegrass through a low-moisture anhydrous ammonia (LMAA) pretreatment and simultaneous saccharification and co-fermentation (SSCF) was examined. At first, Italian ryegrass was subjected to LMAA pretreatment, which was performed by keeping the moist powdered Italian ryegrass under NH3 gas atmosphere at room temperature for 4 weeks. The effect of LMAA pretreatment was examined for bio-ethanol production through simultaneous saccharification and fermentation (SSF) which was performed at 34 °C using Saccharomyces cerevisiae, cellulase, and xylanase. The yields of ethanol and xylose in SSF were enhanced by LMAA-pretreatment. The SSCF of the LMAA-retreated Italian ryegrass was performed at 36 °C using Escherichia coli KO11, S. cerevisiae, cellulase, and xylanase. The SSCF of LMAA-pretreated Italian ryegrass produced ethanol in higher yield (84.6 %) compared with those of LMAA-pretreated bamboo, rice straw, Napier grass, and silvergrass.

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Abbreviations

AFEX:

Ammonia fiber explosion pretreatment

CF:

Co-fermentation

E:

Ethanol

G:

Glucose

HF:

Hexose fermentation

LC:

Lignocellulosic materials

LMAA:

Low-moisture anhydrous ammonia pretreatment·

SAA:

Soaking in aqueous ammonia pretreatment

SSF:

Simultaneous saccharification and fermentation

SSCF:

Simultaneous saccharification and co-fermentation

PT:

Pretreatment

X:

Xylose

XF:

Xylose fermentation

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Acknowledgments

This study was supported by a Grant-in-Aid for Scientific Research (C) No 24610055 from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.

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Authors and Affiliations

  1. Department of Applied Chemistry, Faculty of Engineering, University of Miyazaki, Gakuen-Kibanadai Nishi, Miyazaki, 889-2192, Japan

    Masahide Yasuda, Yuka Takenouchi & Yui Nitta

  2. Department of Animal and Grassland Sciences, Faculty of Agriculture, University of Miyazaki, Gakuen-Kibanadai Nishi, Miyazaki, 889-2192, Japan

    Yasuyuki Ishii

  3. Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, Gakuen-Kibanadai Nishi, Miyazaki, 889-2192, Japan

    Kazuyoshi Ohta

Authors
  1. Masahide Yasuda
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  2. Yuka Takenouchi
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  3. Yui Nitta
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  4. Yasuyuki Ishii
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  5. Kazuyoshi Ohta
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Correspondence to Masahide Yasuda.

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Yasuda, M., Takenouchi, Y., Nitta, Y. et al. Italian ryegrass (Lolium multiflorum Lam) as a High-Potential Bio-Ethanol Resource. Bioenerg. Res. 8, 1303–1309 (2015). https://doi.org/10.1007/s12155-015-9582-5

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