Abstract
A label-free biosensor based on surface plasmon resonance (SPR) has been developed to evaluate the potential of peptide ligands as biorecognition molecules for the detection of bacterial toxins. A 24-mer peptide, previously identified from a phage display library and modified for better binding, was used for the detection of staphylococcal enterotoxin B (SEB). The peptide was immobilized covalently onto the gold-coated surface modified with self-assembly monolayer. The limit of detection (LOD) for SEB was found to be 20 μg/mL. The selectivity of the peptide-based sensor for SEB was verified by injecting bovine serum albumin (BSA), and the sensor response for the BSA was negligible. Further, to probe the effectiveness of the sensor in complex food environments, it was tested with milk samples spiked with SEB, showing that it is applicable to complex food matrices. The results of this study demonstrate the potential of peptides, selected from phage display libraries, as recognition agents for detecting bacterial toxins using biosensors.
Similar content being viewed by others
References
Baeriswyl V, Heinis C (2013) ChemMedChem 8:377
Carnazza S, Foti C, Gioffre G, Felici F, Guglielmino S (2008) Biosens Bioelectron 23:1137
Cerruti M, Jaworski J, Raorane D, Zueger C, Varadarajan J, Carraro C, Lee SW, Maboudian R, Majumdar A (2009) Anal Chem 81:4192
Dudak FC, Soykut EA, Oğuz ME, Yaşar F, Boyacı İH (2010) J Mol Recognit 2:369
Dudak FC, Kılıç N, Demir K, Yaşar F, Boyacı İH (2012) Biopolymers 98:145
Goldman ER, Pazirandeh MP, Mauro JM, King KD, Frey JC, Anderson GP (2000) J Mol Recognit 13:382
Goldman ER, Pazirandeh MP, Charles PT, Balighian ED, Anderson GP (2002) Anal Chim Acta 457:13
Hahn IF, Pickenhahn P, Lenz W, Brandis H (1986) J Immunol Methods 92:25
Harteveld JLN, Nieuwenhuizen MS, Wils ERJ (1997) J Microbiol Meth 7:661
Hennekinne JA, De Buyser ML, Dragacci S (2012) FEMS Microbiol Rev 36:815
Homola J, Dostalek J, Chen S, Rasooly A, Jiang S, Yee SS (2002) Int J Food Microbiol 7:61
Huan TN, Ha VTT, Hung LQ, Yoon MY, Han SH, Chung H (2009) Biosens Bioelectron 25:469
Iqbal SS, Mayo MW, Bruno JG, Bronk BV, Batt CA, Chambers JP (2000) Biosens Bioelectron 15:549
Ivnitski D, Abdel-Hamid I, Atanasov P, Wilkins E (1999) Biosens Bioelectron 14:599
King KD, Anderson GP, Bullock KE, Regina MJ, Saaski EW, Ligler FS (1999) Biosens Bioelectron 14:163
Lavilla M, Moros M, Puertas S, Grazú V, Pérez MD, Calvo M, de la Fuente JM, Sánchez L (2012) Anal Bioanal Chem 402:3219
Lin HC, Tsai WC (2003) Biosens Bioelectron 18:1479
Marasco D, Perretta G, Sabatella M, Ruvo M (2008) Curr Protein Pept Sci 9:447
Marrack P, Kappler J (1990) Science 248:705
Medina MD (2003) J Rapid Meth Autom Microbiol 11:225
Naimushin AN, Soelberg SD, Nguyen DK, Dunlap L, Bartholomew D, Elkind J, Melendez J, Furlong CE (2002) Biosens Bioelectron 17:573
Nanduri V, Balasubramanian S, Sista S, Vodyanoy VJ, Simonian AL (2007) Anal Chim Acta 589:166
Olsen EV, Sorokulova IB, Petrenko VA, Chen IH, Barbaree JM, Vodyanoy VJ (2006) Biosens Bioelectron 21:1434
Park CE, Akhtar M, Rayman MK (1994) Appl Environ Microbiol 60:677
Pavan S, Berti F (2012) Anal Bioanal Chem 402:3055
Petrenko VA, Smith GP, Gong X, Quinn T (1996) Protein Eng 9:797
Rajkovic A, El-Moualij B, Uyttendaele M, Brolet P, Zorzi W, Heinen E, Foubert E, Debevere J (2006) Appl Environ Microbiol 72:6593
Rasooly A (2001) J Food Protect 64:37
Rastelli L, Valentino ML, Minderman MC, Landin J, Malyankar UM, Lescoe MK, Kitson R, Brunson K, Souan L, Forenza S, Goldfarb RH, Rabbani SA (2011) Int J Oncol 39:401
Righetti PG, Boschetti E, Kravchuk AV, Fasoli E (2010) Expert Rev Proteomics 7:373
Samoylov AM, Samoylova TI, Pathirana ST, Globa LP, Vodyanoy VJ (2002a) J Mol Recognit 15:197
Samoylov AM, Samoylova TI, Hartell MG, Pathirana ST, Smith BF, Vodyanoy V (2002b) Biomol Eng 18:269
Smith GP (1985) Science 228:1315
Tsai WC, Pai PJR (2009) Microchim Acta 166:115
Van Dorst B, Mehta J, Bekaert K, Rouah-Martin E, De Coen W, Dubruel P, Blust R, Robbens J (2010) Biosens Bioelectron 26:1178
Wu J, Cropek DM, West AC, Banta S (2010) Anal Chem 82:8235
Wu J, Park JP, Dooley K, Cropek DM, West AC, Banta S (2011) PLoS One 6:e24948
Zhu HY, White IM, Suter JD, Fan XD (2008) Biosens Bioelectron 24:461
Acknowledgments
This study has been financially supported by the Scientific and Technological Research Council of Turkey (TUBITAK) with the project number 108O031.
Conflict of Interest
Fahriye Ceyda Dudak declares that she has no conflict of interest. İsmail Hakkı Boyacı declares that he has no conflict of interest. This article does not contain any studies with human or animal subjects.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Dudak, F.C., Boyaci, İ.H. Peptide-Based Surface Plasmon Resonance Biosensor for Detection of Staphylococcal Enterotoxin B. Food Anal. Methods 7, 506–511 (2014). https://doi.org/10.1007/s12161-013-9739-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12161-013-9739-9