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Preparation of Ofloxacin Poly(Glycidyl Methacrylate-Co-Ethylenedimethacrylate) (PGMA/EDMA) Molecularly Imprinted Microspheres and Their Application to the Analysis of Quinolones in Milk

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Abstract

A series of novel ofloxacin-imprinted polymers have been prepared using poly(glycidyl methacrylate-co-ethylenedimethacrylate) (PGMA/EDMA) particles as a support and their recognition abilities evaluated. Several factors that are known to influence the rebinding of the imprinted polymers were investigated, including the pH and water content of the adsorbed solutions. The binding capabilities of the materials were also evaluated. The resulting PGMA/EDMA molecularly imprinted polymers were used as selective solid phase extraction sorbents for the simultaneous determination of three different quinolones in milk, with the results revealing high levels of selectivity. The use of 10 % methanol–water as washing solution together with 2 % trifluoroacetic acid–acetonitrile as an elution solution allowed for the three different quinolones to be selectively extracted from the milk samples. Furthermore, all of the matrix interference factors were eliminated simultaneously, whereas the enrichment factors were >234. The quantification limit was determined to be 1.00 ng/mL using an ultraviolet detector, and the recovery was in the range of 81.8–102.0 % with a relative deviation of <9.6 %.

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Acknowledgments

The authors would like to thank the National Natural Science Foundation of China for financially supporting this research (grant no. 81173025).

Conflict of Interest

Jing Zhang has no conflict of interest. Ling-Ling Wang has no conflict of interest. Jin-Qin Ma has no conflict of interest. Yan-Li Wang has no conflict of interest. The institutes where the authors work also agree to the submission of this paper to the journal. Jing Zhang has received research grants from National Natural Science Foundation of China. All authors have read and approved this version of the article, and due care has been taken to ensure the integrity of the work. More importantly, this article does not contain any studies with human or animal subjects. The institutes where the authors work also agree to the submission of this paper to the journal.

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Authors and Affiliations

  1. Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi’an, 710062, People’s Republic of China

    Jing Zhang, Ling-Ling Wang, Jin-Qin Ma & Yan-Li Wang

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  1. Jing Zhang
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  2. Ling-Ling Wang
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Correspondence to Jing Zhang.

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Zhang, J., Wang, LL., Ma, JQ. et al. Preparation of Ofloxacin Poly(Glycidyl Methacrylate-Co-Ethylenedimethacrylate) (PGMA/EDMA) Molecularly Imprinted Microspheres and Their Application to the Analysis of Quinolones in Milk. Food Anal. Methods 7, 721–729 (2014). https://doi.org/10.1007/s12161-013-9754-x

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  • DOI: https://doi.org/10.1007/s12161-013-9754-x

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