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Xylanases of anaerobic fungus Anaeromyces mucronatus

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Abstract

The anaerobic fungus Anaeromyces mucronatus KF8 grown in batch culture on M10 medium with rumen fluid and microcrystalline cellulose as carbon source produced a broad range of enzymes requisite for degradation of plant structural and storage saccharides including cellulase, endoglucanase, xylanase, α-xylosidase, β-xylosidase, α-glucosidase, β-glucosidase, β-galactosidase, mannosidase, cellobiohydrolase, amylase, laminarinase, pectinase and pectate lyase. These enzymes were detected in both the intra- and extracellular fractions, but production into the medium was prevalent with the exception of intracellular β-xylosidase, chitinases, N-acetylglucosaminidase, and lipase. Xylanase activity was predominant among the polysaccharide hydrolases. Extracellular production of xylanase was stimulated by the presence of cellobiose and oat spelt xylan. Zymogram of xylanases of strain KF8 grown on different carbon sources revealed several isoforms of xylanases with approximate molar masses ranging from 26 to 130 kDa.

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Abbreviations

AZO:

azocasein

CM-xylan:

6-O-carboxymethylxylan

SDS:

sodium dodecyl sulfate

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Authors and Affiliations

  1. Institute of Animal Physiology and Genetics, v.v.i., Academy of Sciences of the Czech Republic, 142 20, Prague 4, Czech Republic

    Z. Novotná, J. Šimůnek & K. Fliegerová

  2. Institute of Chemical Technology, 166 28, Prague 6, Czech Republic

    J. Procházka

  3. Department of biochemistry, Faculty of Science, Charles University in Prague, 128 43, Prague 2, Czech Republic

    Z. Novotná

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  1. Z. Novotná
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  2. J. Procházka
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  3. J. Šimůnek
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  4. K. Fliegerová
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Correspondence to Z. Novotná.

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Novotná, Z., Procházka, J., Šimůnek, J. et al. Xylanases of anaerobic fungus Anaeromyces mucronatus . Folia Microbiol 55, 363–367 (2010). https://doi.org/10.1007/s12223-010-0059-9

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  • DOI: https://doi.org/10.1007/s12223-010-0059-9

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