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Optimization of nutrient medium containing agricultural waste for xylanase production by Bacillus pumilus B20

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Abstract

We aimed to optimize a nutrient medium containing agricultural waste for xylanase production by Bacillus pumilus B20. Xylanase production with lignocellulosic material was optimized in two steps using DeMeo’s fractional factorial design. A 3.4-fold increase in xylanase production (313.3 U/mL) was achieved using the optimized culture medium consisting of (g/L): K2HPO4, 2; MgSO4·7H2O, 0.3; CaCl2·2H2O, 0.01; NaCl, 2; peptone, 5 yeast extract, 4; and wheat bran, 50. B. pumilus B20 produced a high level of xylanase, which may have potential industrial application.

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  1. Department of Genetics, Centre for Excellence in Genomic Sciences, School of Biological Sciences, Madurai Kamaraj University, Madurai, India

    K. Geetha & P. Gunasekaran

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  2. P. Gunasekaran
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Geetha, K., Gunasekaran, P. Optimization of nutrient medium containing agricultural waste for xylanase production by Bacillus pumilus B20. Biotechnol Bioproc E 15, 882–889 (2010). https://doi.org/10.1007/s12257-009-3094-0

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