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Chymotrypsin — Eudragit® complex formation

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Abstract

Eudragit® L100 (EuL) and Eudragit® S100 (EuS) are synthetic polyanions differing on their electric charge density. They interact with chymotrypsin (ChTRP), a basic protein forming soluble and non-soluble complexes. The complex formation was studied by dynamic light scattering, isothermal titration calorimetry, native fluorescence emission, circular dichroism and thermodynamical thermal stability of the enzyme. EuS was able to bind 33 ChTRP molecules while EuL, 60. The binding of ChTRP to both Eu was slightly endothermic and the entropic factor was responsible for the soluble complexes formation. The ChTRP-Eu size increases with pH and the binding of ChTRP to Eu modifies the Eu hydrodynamic radium. The interaction of ChTRP with Eu did not modify its secondary or tertiary structure. The thermal stability of ChTRP was increased when it interacted with both Eu.

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Authors and Affiliations

  1. Laboratorio de Fisicoquímica Aplicada a Bioseparación, Departamento de Química-Física, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 570 (S2002RLK), Rosario, Argentina

    Valeria Boeris, Laura Verónica Cappella & Guillermo Picó

  2. Instituto de Química, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9500, Agronomia, 91501-970, Porto Alegre, RS, Brasil

    Gisele Peres & Nádya Pesce da Silveira

  3. Departamento de Química Biológica, Centro de Investigaciones en Química Biológica Facultad de Ciencias Químicas, Universidad Nacional de Coìrdoba, Córdoba, Argentina

    Inés Burgos & Gerardo Fidelio

Authors
  1. Valeria Boeris
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  2. Laura Verónica Cappella
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  3. Gisele Peres
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  4. Inés Burgos
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  5. Nádya Pesce da Silveira
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  6. Gerardo Fidelio
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  7. Guillermo Picó
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Correspondence to Guillermo Picó.

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Boeris, V., Cappella, L.V., Peres, G. et al. Chymotrypsin — Eudragit® complex formation. Biotechnol Bioproc E 18, 538–545 (2013). https://doi.org/10.1007/s12257-012-0553-9

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  • DOI: https://doi.org/10.1007/s12257-012-0553-9

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