Abstract
The present research work highlighted the possibility of exploiting marine microbes for the biosynthesis of copper nanoparticles and further investigated thermodynamic changes during the biomineralization of copper in a microbial system by isothermal titration calorimetry. A bacterial strain (M-7) isolated from Kanyakumari coast, India was found capable of synthesizing these nanoparticles. Based on phylogenetic relationship, the strain M-7 was identified as Kocuria flava. The nanoparticles produced were characterized using UV-vis spectrophotometry, Transmission electron microscopy (TEM) and Dynamic light scattering (DLS). Data revealed the formation of spherical and quasi-spherical shaped nanoparticles with an average particle size ranging between 5 and 30 nm. Additionally, a study on the effect of different media components indicated that media containing higher amount of casein enzymic hydrolysate and yeast extract supported the formation of stable colloidal suspension of nanoparticles. Finally, isothermal titration calorimetry (ITC) was carried out to understand the change in heat energy during the formation of nanoparticles in different media. Combinatorial observations of all these studies may open up new strategies to develop tailor made copper nanoparticles via green route.
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Sau, T. K., A. L. Rogach, F. Jackel, T. A. Klar, and J. Feldmann (2010) Properties and applications of colloidal nonspherical noble metal nanoparticles. Adv. Mater. 22: 1805–1825.
Syed, A., S. Saraswati, G. C. Kundu, and A. Ahmad (2013) Biological synthesis of silver nanoparticles using the fungus Humicola sp. and evaluation of their cytoxicity using normal and cancer cell lines. Spectrochim Acta A. 114: 144–147.
Ai, J., E. Biazar, M. Jafarpour, M. Montazeri, A. Majdi, S. Aminifard, M. Zafari, H. R. Akbari, and H. G. Rad (2011) Nanotoxicology and nanoparticle safety in biomedical designs. Int. J. Nanomed. 6: 1117–1127.
Mukherjee, P., M. Roy, B. P. Mandal, G. K. Dey, P. K. Mukherjee, J. Ghatak, A. K. Tyagi, and S. P. Kale (2008) Green synthesis of highly stabilized nanocrystalline silver particles by a non-pathogenic and agriculturally important fungus T. asperellum. Nanotechnol. 19: 75103–75110.
Narayanan, K. B. and N. Sakthivel (2010) Biological synthesis of metal nanoparticles by microbe. Adv. Coll. Interf. Sci. 156: 1–3.
Borkow, G., J. Gabbay, R. Dardik, A.L. Eidelman, Y. Lavie, Y. Grunfeld, S. Ikher, M. Huszar, R. C. Zatcoff, and M. Marikovsky (2010) Molecular mechanisms of enhanced wound healing by copper oxide-impregnated dressings. Wound Rep. Regen. 18: 266–275.
Hassan, S. S., S. Singh, R. Y. Parikh, M. S. Dharne, M. S. Patole, B. L. Prasad, and Y. S. Shouche (2008) Bacterial synthesis of copper/copper oxide nanoparticles. J. Nanosci. Nanotechnol. 8: 3191–3196.
Singh, V. A., R. Patil, A. Anand, M. Paolo, and W. N. Gade (2010) Biological synthesis of copper oxide nano particles using Escherichia coli. Curr. Nanosci. 6: 365–369.
Ramanathan, R., S. K. Bhargava, and V. Bansal (2011) Biological synthesis of copper/copper oxide nanoparticles. pp. 1–8. In: Rose Amal (ed.). CHEMECA 2011 — Engineering a better world, Engineers Australia.
Soheyla, H., H. Barabadi, E. Gharaeifathabad, and F. Naghibi (2012) Green synthesis of copper oxide nanoparticles using Penicillium aurantiogriseum, Penicillium citrinum and Penicillium waksmanii. Dig. J. Nanomater. Biostruc. 7: 999–1005.
Sharma, N., A. K. Pinnaka, M. Raje, Ashish, M. S. Bhattacharya, and A. R. Choudhury (2012) Exploitation of marine bacteria for production of gold nanoparticles. Microb. Cell Fact. 11: 86.
Asmathunisha, N. and K. Kathiresan (2013) A review on biosynthesis of nanoparticles by marine organisms. Coll. Surf. B: Biointerf. 103: 283–287.
Atkins, W. R. G. (1932) The copper content of sea-water. J. Mar. Biol. Assoc. U. K. 18: 193–197.
Murray, R. G. E., R. N. Doetsch, and F. Robinow (1994) Determinative and cytological light microscopy. pp. 21–41. In: P. Gerhard, R. G. E. Murray, W. A. Wood, and N. R. Krieg (eds.). In Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
Cowan, S. T. and K. J. Steel (1965) Manual for the identification of Medical Bacteria. Cambridge University Press, London, UK.
Smibert, R. M. and N. R. Krieg (1994) Phenotypic characterization. pp. 607–654. In: P. Gerhardt and D. C. Washington (eds.). In Methods for General and Molecular Bacteriology. American Society for Microbiology.
Lanyi, B. (1987) Classical and rapid identification methods for medically important bacteria. Methods Microbiol. 19: 1–67.
Smith, N. R., R. E. Gordon, and F. E. Clark (1952) Aerobic spore forming bacteria. U. S. Dep. Agric. Agriculture Monograph, no. 16.
Mayilraj, S., P. K. Saha, K. Suresh, and H. S. Saini (2006) Ornithinimicrobium kibberense sp. nov., isolated from the Himalayas, India. Int. J. Syst. Evol. Microbiol. 56: 1657–1661.
Kim, O., Y. J. Cho, K. Lee, S. H. Yoon, M. Kim, H. Na, S. C. Park, Y. S. Jeon, J. H. Lee, H. Yi, S. Won, and J. Chun (2012) Introducing EzTaxon-e: A prokaryotic 16S rRNA Gene sequence database with phylotypes that represent uncultured species. Int. J. Syst. Evol. Microbiol. 62: 716–721.
Tamura, K., D. Peterson, N. Peterson, G. Stecher, M. Nei, and S. Kumar (2011) MEGA5: Evolutionary genetics analysis using maximum likelihood, evolutionary distance and maximum parsimony methods. Mol. Biol. Evol. 28: 2731–2739.
Mandel, M. and J. Marmur (1968) Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol. 12 B: 195–206.
Malhotra, A., K. Dolma, N. Kaur, Y. S. Rathore, Ashish, S. Mayilraj, and A. R. Choudhury (2013) Biosynthesis of gold and silver nanoparticles using a novel marine strain of Stenotrophomonas. Bioresour. Technol. 142: 727–731.
Abboud, Y., T. Saffaj, A. Chagrouni, A. El-Bourari, K. Brouzi, O. Tanane, and B. Ihssane (2013) Biosynthesis, characterization and antimicrobial activity of copper oxide nanoparticles (CONPs) produced using brown alga extract (Bifurcaria bifurcata). Appl. Nanosci. 4: 571–576.
Rahman, A., A. Ismail, D. Jumbianti, S. Magdalena, and H. Sudrajat (2009) Synthesis of copper oxide nanoparticles by using Phormidium cyanobacterium. Indo. J. Chem. 9: 355–360.
Dang, T. M. D., T. T. T. Le, E. Fribourg-Blanc, and M. C. Dang (2011) Synthesis and optical properties of copper nanoparticles prepared by a chemical reduction method. Adv. Nat. Sci. Nanosci. Nanotech. 2: 015009.
Krimm, S. and J. Bandekar (1986) Vibrational spectroscopy and conformation of peptides, polypeptides, and proteins. Adv. Prot. Chem. 38: 181–367.
Zhang, Y. X., J. Zheng, G. Gao, Y. F. Kong, X. Zhi, K. Wang, X. Q. Zhang, and D. X. Cui (2011) Biosynthesis of gold nanoparticles using chloroplasts. Int. J. Nanomed. 6: 2899–2906.
Salvadori, M. R., L. F. Lepre, R. A. Ando, C. A. O. Nascimento, and B. Correa (2013) Biosynthsis and uptake of copper nanoparticles by dead biomass of Hypocrea lixii isolated from the metal mine in the Brazilian amazon region. Plos One 8: 1–8.
Castro, L., M. L. Blázquez, F. González, J. A. Muñoz, and A. Ballester (2010) Extracellular biosynthesis of gold nanoparticles using sugar beet pulp. Chem. Eng. J. 164: 92–97.
Rajamanickam, K., S. S. Sudhaa, M. Francisa, T. Sowmyaa, J. Rengaramanujama, P. Sivalingam, and K. Prabakar (2013) Microalgae associated Brevundimonas sp. MSK 4 as the nano particle synthesizing unit to produce antimicrobial silver nanoparticles. Spectrochim Acta A. 113: 10–14.
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Kaur, H., Dolma, K., Kaur, N. et al. Marine microbe as nano-factories for copper biomineralization. Biotechnol Bioproc E 20, 51–57 (2015). https://doi.org/10.1007/s12257-014-0432-7
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DOI: https://doi.org/10.1007/s12257-014-0432-7